IN VIVO STUDY ON DIURETIC AND LAXATIVE THERAPEUTIC PROPERTIES OF HYDRO - ALCOHOLIC EXTRACT OF LU HUI (LILLIACEAE) ON EXPERIMENTAL RODENTS (RATTUS NORVEGICUS).
Abstract
Objective: In furtherance to the rising evidences for therapeutical potential of Lu Hui (Aloe vera), the existing study was carried out to evaluate the
diuretic and laxative activity of its hydro-alcoholic extract on rodents.
Materials and Methods: The hydro-alcoholic extract of leaves of Lu Hui (HALL) was prepared by using Soxhlet extractor and subjected to analysis by
standard preliminary phytochemical tests. Assessment of both the diuretic and laxative activity was carried out using standard methods. Furosemide
(20 mg/kg) was functioned as a positive control for diuretic activity, whereas gaviscon (10 mg/kg) worked as a reference drug for laxative activity.
Results: The HALL showed weight diuretic activity and found to be the most potent in increasing the urinary output at 600 mg/kg when the effect
was comparable to that of the standard furosemide. Besides, this extract found to be most effective in increasing urinary electrolyte concentration
(sodium, potassium, chloride) at both the doses tested. On the other hand, the results for laxative activity exhibited incredible increase of feces output
at the dose of 600 mg/kg, and the increase was similar to that of standard drug gaviscon.
Conclusion: Altogether, the above major findings validate and support its folkloric diuretic and purgative use and lend pharmacological credence to
the ethnomedical use of this leaves in the traditional system of medicine, stresses further studies to intricate its active constituents, uses and safety.
Keywords: Bioassay, Diuretic, Laxative, Furosemide, Gaviscon, Ethnomedicine.
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References
Goodman LS, Hardman JG, Limbird LE. Goodman and Gilmans.
The Pharmacological Basis of Therapeutics. 10th International ed.
New York: McGraw Hill; 2001. p. 757-63.
Bosshard W, Dreher R, Schnegg JF, Büla CJ. The treatment of
chronic constipation in elderly people: An update. Drugs Aging
;21(14):911-30.
Mostafa SM, Bhandari S, Ritchie G, Gratton N, Wenstone R.
Constipation and its implications in the critically ill patient. Br J
Anaesth 2003;91(6):815-9.
Johanson JF, Kralstein J. Chronic constipation: A survey of the patient
perspective. Aliment Pharmacol Ther 2007;25(5):599-608.
Shelton RM. Aloe vera. Its chemical and therapeutic properties. Int J
Dermatol 1991;30(10):679-83.
Haller JS Jr. A drug for all seasons. Medical and pharmacological
history of aloe. Bull N Y Acad Med 1990;66(6):647-59.
Atherton P. Aloe vera: Magic or medicine? Nurs Stand 1998;12(49):49-
, 54.
Bensky D, Gamble A, Kaptchuk TJ. Chinese Herbal Medicine: Materia
Medica. Vol. XXV. Seattle, Wash: Eastland Press; 1993. p. 556.
Ghazanfar SA. Handbook of Arabian Medicinal Plants. Boca Raton:
CRC Press; 1994.
Sofowora A. Medical Plants and Traditional Medicine in Africa. 2nd ed.
Ibadan, Nigeria: Spectrum Books Ltd.; 1993. p. 289.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 12th ed. Pune:
Nirali Prakashan; 1999. p. 254.
Khandelwal KR. Practical Pharmacognosy. New Delhi: Nirali
Prakashan; 2006.
Ecobichon DJ. The Basis of Toxicity Testing. New York: CRS Press;
Murugesan T, Manikandan L, Suresh KB, Pal M, Saha BP. Evaluation
of diuretic potential of Jussiaea suffruticosa Linn. extract in rats. Indian
J Pharm Sci 2000;62(2):150-1.
Rizvi SH, Shoeb A, Kapil RS, Satya P. Two diuretic triterpenoids from
Antidesma menasu. Phytochemistry 1980;19(11):2409-10.
Jeffery GH, Bassett J, Mendham J, Denny RC. Vogel’s Textbook of
Quantitative Chemical Analysis. 5th ed. England: Addison Wesley
Longman Ltd.; 1989. p. 801.
Beckette AH, Stenlake JB. Practical Pharmaceutical Chemistry. Part-I.
st ed. New Delhi: CBS Publishers & Distributors; 1997. p. 197.
Capasso F, Mascolo N, Autore G, Romano V. Laxatives and
the production of autacoids by rat colon. J Pharm Pharmacol
;38(8):627-9.
Ganapaty S, Dash GK, Subburaju T, Suresh P. Diuretic, laxative
and toxicity studies of Cocculus hirsutus aerial parts. Fitoterapia
;73(1):28-31.
Reynolds T, Dweck AC. Aloe vera leaf gel: A review update.
J Ethnopharmacol 1999;68(1-3):3-37.
Guyton AC, Hall JE. Textbook of Medical Physiology. 9th ed. Singapore:
WB Saunders Company; 1998. p. 306-8.
Basu SK, Arivukkarasu R. Acute toxicity and diuretic studies of Rungia
repens aerial parts in rats. Fitoterapia 2006;77(2):83-5.
Hughes S, Higgs NB, Turnberg LA. Loperamide has antisecretory
activity in the human jejunum in vivo. Gut 1984;25(9):931-5.
Aikawa N, Ohmori K. Effect of zaldaride maleate, an antidiarrheal
compound, on fecal pellet output induced by hyperpropulsion in
gastrointestine of rats. Jpn J Pharmacol 2000;82(4):350-2.
Yamada K, Onoda Y. Comparison of the effects of T-1815, yohimbine
and naloxone on mouse colonic propulsion. J Smooth Muscle Res
;29(2):47-53.
Schiller LR, Santa Ana CA, Morawski SG, Fordtran JS. Mechanism
of the antidiarrheal effect of loperamide. Gastroenterology
;86(6):1475-80.
Shimotoyodome A, Meguro S, Hase T, Tokimitsu I, Sakata T. Decreased
colonic mucus in rats with loperamide-induced constipation. Comp
Biochem Physiol A Mol Integr Physiol 2000;126(2):203-12.
Wintola OA, Sunmonu TO, Afolayan AJ. The effect of Aloe ferox Mill.
in the treatment of loperamide-induced constipation in wistar rats.
BMC Gastroenterol 2010;10:95.
Ishii Y, Tanizawa H, Takino Y. Studies of aloe. V. Mechanism of
cathartic effect. (4). Biol Pharm Bull 1994;17(5):651-3.
Izzo AA, Sautebin L, Borrelli F, Longo R, Capasso F. The role of
nitric oxide in aloe-induced diarrhoea in the rat. Eur J Pharmacol
;368(1):43-8.
Ishii Y, Tanizawa H, Takino Y. Studies of aloe. III. Mechanism of
cathartic effect. (2). Chem Pharm Bull (Tokyo) 1990;38(1):197-200.
Capasso F, Mascolo N, Autore G, Duraccio MR. Effect of indomethacin on
aloin and 1, 8 dioxianthraquinone-induced production of prostaglandins
in rat isolated colon. Prostaglandins 1983;26(4):557-62.
Niwa T, Nakao M, Hoshi S, Yamada K, Inagaki K, Nishida M, et al.
Effect of dietary fiber on morphine-induced constipation in rats. Biosci
Biotechnol Biochem 2002;66(6):1233-40.
Dande P, Vaidya A, Arora P. Laxative activity of Raphanus sativus L.
leaf. Asian J Pharm Clin Res 2014;7 (Suppl 2):120-4.
Degen L. Gastrointestinal motility – Physiology and methods of
measurement. Ther Umsch 2007;64(4):195-9.
Capasso F, Gaginella TS. Laxatives: A Practical Guide. Milan: Springer
Italia; 1997.
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