PHYTOCHEMICAL PROFILE STUDIES ON THE STEROIDS OF METHANOLIC LEAF EXTRACT OF MEDICINALLY IMPORTANT PLANT Holoptelea Integrifolia (Roxb.) PLANCH USING HPTLC
Abstract
Objective: The present study was conducted to identify the steroids from the methanol extract of medicinally and economically useful leaves of
Holoptelea integrifolia (Roxb.) planch using high-performance thin layer chromatography (HPTLC) technique.
Materials and Methods: Preliminary phytochemical screening was done, and HPTLC studies were carried out. Camag HPTLC system equipped
with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at
540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp.
Results: Preliminary phytochemical screening of methanolic extract of H. integrifolia showed the presence of steroids, alkaloids, flavonoids,
proteins, and carbohydrates. HPTLC fingerprinting of steroids of methanol extract of leaf showed seven polyvalent phytoconstituents (7 peaks) and
corresponding ascending order of radio frequency (Rf) values in the range of 0.17-0.64.
Conclusions: With the above Rf values and preliminary phytochemical analysis, we have concluded the presence of steroids in methanol extract.
Keywords: Holoptelea integrifolia (Roxb.) planch leaf, Phytochemical screening, Steroids, High-performance thin layer chromatography
fingerprinting.
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References
Kiruba S, Mahesh M, Nisha SR, Miller Paul Z, Jeeva S. Phytochemical
analysis of the flower extracts of Rhododendron arboretum Sm. Ssp.
Nilagiricum (Zenker) Tagg. Asian Pac J Trop Biomed 2011;???:284-6.
Tirupathi RG, Suresh BK, Ujwal KJ, Sujana P, Raoa AV, Sreedhar AS.
Anti-microbial principles of selected remedial plants from Southern
India. Asian Pac J Trop Biomed 2011;1(4):298-305.
Raja RD, Jeeva S, Prakash JW, Antonisamy JM,
Irudayaraj V. Antibacterial activity of selected ethnomedicinal plants
from South India. Asian Pac J Trop Med 2011;4(5):375-8.
Robonson MM, Zhang X. The World Medicine Situation, Traditional
Medicine: Global Situation, Issues and Challenges. 3rd ed. Geneva:
WHO; 2011.
Newman DJ, Cragg GM. Natural products as sources of new drugs over
the 30 years from 1981 to 2010. J Nat Prod 2012;75(3):311-35.
Osbourn AE, Lanzotti V. Plant Derived Natural Products. Synthesis
Function and Application; New York: Springer Science; 2009.
Parekh J, Chanda S. Phytochemical Screenig of some plants from
western region of India. Plant Arch 2008;8(2):657-62.
Nadkarni KM. Indian Materia Medica. Mumbai, India: Popular
Prakashan Pvt. Ltd.; 1976. p. 651-2.
Kirtikar KR, Basu BD. Indian Medicinal Plants. 3rd ed., Vol. III.
New Delhi, India: Sri Satguru Publications; 2000. p. 2292-4.
Prajapati ND, Purohit SS, Sharma AK. AHandbook of Medicinal Plants
a Complete Source Book. Jodhpur, India: Agrobias; 2003. p. 273.
Warrier PK, Nambiar VP, Ramakutty C. Indian Medicinal Plants a
Compendium of 500 Species. Vol. 3. Hyderabad: Orient Longman
Private Limited; 1995. p. 162.
Khandelwal KR. Practical Pharmacognosy. Techniques and
Experiments. 10th ed. Pune, India: Nirali Prakashan; 2006. p. 149-56.
Harborne JB. Phytochemical Methods. 3rd ed. London: Chapman and
Hall; 1998
Wagner H, Baldt S. Plant Drug Analysis. Berlin: Springer;
Scott RP. Encyclopedia of Chromatography. 10th ed.
USA: Marcel Dekker; 2001. p. 252-4.
ICH/CPMP Guidelines Q2B, Validation of Analytical Procedures–
Methodology, 1996.
Cazes J, Scott RP. Chromatography Theory. NY: Marcel Decker; 2002.
p. 443‑54.
Reviewer Guidance, Validation of Chromatographic Methods.
???: Center for Drug Evaluation and Research, US Food and Drug
Administration; 1994
Sethi PD. HPTLC: Quantitative Analysis of Pharmaceutical
Formulations. New Delhi: CBS Publications; 1996. p. 162-5.
Heftman E. Chromatography Fundamentals and Applications of
Chromatography and Related Differential Migration Methods. 6th ed.,
Vol. 69A. Amsterdam: Elsevier; 2004. p. 253-91.
British Pharmacopoeia. International ed., Vol. II. Cambridge: HMSO;
Appendix 112 (IB).
Sherma J. Encyclopedia of Pharmaceutical Technology. 2nd ed. USA:
Marcel Dekker; 2001. p. 252-4.
ICH/CPMP Guidelines Q2A, Text on Validation of
Analytical Procedures; 1994.
???. USP 23, NF 19, Asian ed. Rockville, M.D: United States
Pharmacopeial Convention; 1982. p. 1225.
Hariprasad PS, Ramakrishnan N. HPTLC fingerprint profile of Rumex
Vesicarius L. Asian J Pharm Clin Res 2011;4(2):134-6.
Swarnalatha Y, Kommineni L. Finger printing analysis of the alkaloids
from Sphaeranthus amaranthoides leaves using HPTLC analysis.
Asian J Pharm Clin Res 2014;7(2):125-7.
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