OPTIMATION OF ETHANOL CONCENTRATION IN EXTRACTION OF EUGENOL FROM GALANGAL RHIZOME

Adi Yugatama; Nur Wahida Ardiyati; Ira Yulianti

doi:10.22159/ajpcr.2017.v10s2.19474

Authors

Adi Yugatama Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Indonesia.
Nur Wahida Ardiyati Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Indonesia.
Ira Yulianti Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s2.19474

Keywords:

Eugenol, Galangal, Extraction, Densitometer

Abstract

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Â Objective: The main objective of this study was to determine the optimal ethanol concentration as solvent for extracting eugenol from galangal.

Methods: Galangal rhizome extraction was conducted with kinetic maceration at 50Â°C with various ethanol concentration as solvent (0%, 30%, 50%, 70%, and 96%). A 1:10 ratio of rhizome and ethanol was applied. The extract was obtained, then its eugenol concentration was analyzed qualitatively and quantitatively by thin-layer chromatography-densitometer with n-hexane:ethyl acetate (4:1) by using mobile phase and silica gel 60 F254 as stationary phase and applying a wavelength of 283 nm.

Results: The result of qualitative analysis showed that extract of 70% and 96% ethanol had a spot with Rf value 0.63, which was equal to the Rf value of standard eugenol. Standard curve equation for the 70% ethanol extract was y=89318x+656.07 (r=0.9993) and for the 96% ethanol extract was y=8658x+1743 (r=0.9999). The result of the quantitative analysis showed that the 70% and 96% ethanol extract contained 4.85% and 4.79% eugenol, respectively.

Conclusion: Extraction of galangal rhizome in 70% and 96% ethanol was positively containing eugenol. Highest eugenol concentration (4.85%) was obtained from galangal rhizome extraction in 70% ethanol.

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