@article{PATEL_PATEL_PATEL_2020, title={DEVELOPMENT AND VALIDATION OF REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF OLANZAPINE AND ARIPIPRAZOLE IN SYNTHETIC MIXTURES}, volume={13}, url={https://journals.innovareacademics.in/index.php/ajpcr/article/view/38278}, DOI={10.22159/ajpcr.2020.v13i8.38278}, abstractNote={<p><strong>Objective: </strong>A simple, rapid, accurate, precise, specific, and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method has been developed and validated for simultaneous estimation of olanzapine (OLZ) and aripiprazole (APR) in synthetic mixtures.</p> <p><strong>Methods: </strong>The stationary phase used for chromatographic separation was Phenomenex C18 column (250 mm × 4.6 mm i.d, particle size 5 μm) and mobile phase used for separation was methanol: Phosphate buffer (pH 3) taken in ratio of 75:25 %v/v. The flow rate was used 1.0 ml/min at room temperature and drugs detected at 240 nm with injection volume 20 μL.</p> <p><strong>Results: </strong>The retention time for OLZ and APR was found to be 4.231 and 6.523 min, respectively. The linearity was performed using a concentration range of 0.5–3.0 for both drugs. The correlation coefficient was found to be 0.999 for OLZ and APR. The % purity of both the drug was found to be 98–102%. The proposed RP-HPLC method has been validated, according to International council on harmonization Q2 (B) guidelines.</p> <p><strong>Conclusion: </strong>There was no interference of any diluents and excipients in the determination of drugs from synthetic mixture. Hence, the developed method can be used for routine quality control analysis.</p>}, number={8}, journal={Asian Journal of Pharmaceutical and Clinical Research}, author={PATEL, MEGHA and PATEL, PARESH and PATEL, DHARA}, year={2020}, month={Aug.}, pages={162–168} }