DESIGN, DEVELOPMENT AND CHARACTERIZATION OF CURCUMIN LOADED NANOTABLET IN ENDOMETRIOSIS TREATMENT

UMMANGALBALAN ABHINI; GURUSAMY MARIAPPAN; BHAVNA KUMAR

doi:10.22159/ijap.2025v17i1.52533

Authors

UMMANGALBALAN ABHINI St Mary's College of Pharmacy, Secunderabad, Telangana-500025, India https://orcid.org/0000-0001-7429-8047
GURUSAMY MARIAPPAN St Mary's College of Pharmacy, Secunderabad, Telangana-500025, India https://orcid.org/0000-0002-0790-5545
BHAVNA KUMAR Faculty of Pharmacy, DIT University, Dehradun, Uttarkhand, India

DOI:

https://doi.org/10.22159/ijap.2025v17i1.52533

Keywords:

Endometriosis, Nanosponges, Cyclodextrins, Curcuminoids, Dasatinib Nanobubbles, Chronic myeloid leukemia, Box-Behnken design., Histopathology

Abstract

Objective: The goal was to simplify the manufacture of curcumin-loaded-nanosponges (CUNS) and test their vaginal delivery of CU for endometriosis in mice.

Methods: The independent parameters of CU-β-Cyclodextrin (CU-β-CD) NS generation were improved using Box-Behnken Design (BBD). BBD with three factors and three levels was used for the studies. The study used carbonyldiimidazole as a cross-linking agent and lyophilization to create CU-β-CDNS. The anti-endomtriosis activity of nano-tablet was tested in mice with peritoneal endometriosis.

Results: The mean particle size was 76.78–154.56 nm, and the encapsulation effectiveness was 76.62–86.68%. Transmission Electron Microscopy showed that the polymer encapsulated CU. In-vitro antioxidant activity showed that CU and CUNS had SC₅₀ values of 5243.52 ± 389.92 and 187.36 ± 16.78 μg/mL, respectively. Bio-adhesion studies showed hydroxypropyl methylcellulose and xanthan gum performed better. The F1 and F2 formulations had better in-vitro drug release at 12 hours, with values of 97.12 ± 2.38 and 95.34 ± 3.24%, respectively. Photo stability and simulated intestinal fluid testing were good. Endometriosis mice had leukocyte infiltration and fibrosis, while control mice had increased stromal vessel density and intact epithelium. However, CU nanogel greatly alleviated these issues. Histopathology demonstrated CUNS-pill corrected endometrial pathology.

Conclusion: The study advised CUNS-pill for endometriosis treatment.

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