TY - JOUR AU - Kumala, Shirly AU - Rahmat, Deni AU - Farida, Yunahara AU - Chandra, Benny PY - 2018/11/07 Y2 - 2024/03/29 TI - ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF JAVANESE TURMERIC RHIZOME ENTRAPPTED IN NANOPARTICLES: A NOVEL RULE OF CHITOSAN JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 10 IS - 6 SE - Original Article(s) DO - 10.22159/ijap.2018v10i6.28521 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/28521 SP - 298-302 AB - <p><strong>Objective</strong>:<strong> </strong>The aim of this study was to compare the antibacterial activity of 96% (v/v) ethanolic extract of Javanese turmeric rhizome (<em>Curcuma xanthorrhiza</em> Roxb.) and nanoparticles containing the extract against 4 pathogenic bacteria.</p><p><strong>Methods</strong>:<strong> </strong>The synthesis of chitosan nanoparticles used ionic gelation method. The nanoparticles were evaluated for particle size, zeta potential and morphology. Dilution method was performed to determine minimum inhibitory concentration (MIC).</p><p><strong>Results</strong>:<strong> </strong>The nanoparticles showed particle size of 53.25 nm, polydispersity index of 0.442 and zeta potential value of+31.5 mv. The antibacterial activity results based on the broth dilution method demonstrated that both the extract and the nanoparticles could inhibit the growth of <em>Staphylococcus aureus, Escherichia coli, Salmonella thypi, and Bacillus subtilis</em> in a concentration of 500 µg/ml. The MIC of the extract against <em>Staphylococcus aureus</em> and <em>Bacillus subtilis</em> were 400 µg/ml and against <em>Escherichia coli</em> and <em>Salmonella thypi</em> in a concentration of 500 µg/ml. In addition, the MIC of the nanoparticles against <em>Staphylococcus aureus</em> was 300 µg/ml while against <em>Bacillus subtilis</em> and <em>Salmonella thypi</em> 400 µg/ml and against <em>Escherichia coli</em> 500 µg/ml.</p><p><strong>Conclusion</strong>:<strong> </strong>The nanoparticles have a promising strategy to formulate the extracts in order to decrease the MIC against <em>Staphylococcus aureus</em> and <em>Salmonella thypi</em>.</p> ER -