TY - JOUR AU - Dyta, Viona Prima AU - ., Harmita AU - M, Baitha Palanggatan PY - 2018/12/20 Y2 - 2024/03/29 TI - ANALYSIS OF DISODIUM 5'-GUANYLATE AND DISODIUM 5'-INOSINATE AS FLAVOR ENHANCER IN FOOD SPICES BY THIN-LAYER CHROMATOGRAPHY–DENSITOMETRY JF - International Journal of Applied Pharmaceutics JA - Int J App Pharm VL - 10 IS - 1 SE - Original Article(s) DO - 10.22159/ijap.2018.v10s1.11 UR - https://journals.innovareacademics.in/index.php/ijap/article/view/31416 SP - 51-55 AB - <p><strong>Objective</strong>: The objective of this study was to obtain an optimum and valid method of analysis to determine the levels of disodium 5'-guanylate (DSG)<br />and disodium 5'-inosinate (DSI) in six samples of spices.<br /><strong>Methods</strong>: The optimum method was obtained using silica gel 60 F254 as the stationary phase and isopropanol:water:25% ammonia at a ratio of 6:3:1<br />(v/v) as the mobile phase. The developed spots were scanned using a densitometer in absorbance mode at 260 nm. The methods were valid based<br />on the accuracy criteria (DSG, 99.11–99.96%, and DSI, 98.56–101.05%), precision (DSG, 1.09%, and DSI, 0.49%), and linearity (DSG, r=0.9909, and<br />DSI, r=0.9976).<br /><strong>Results</strong>: The results showed that the levels of DSG in samples A, B, C, D, E, and F were 0.70%, 0.79%, 0.78%, 0.99%, 1.08%, and 1.08% and those of<br />DSI were 0.66%, 0.74%, 0.71%, 0.66%, 0.54%, and 0.67%, respectively.<br /><strong>Conclusion</strong>: The optimum conditions of DSG and DSI for thin-layer chromatography-densitometry were obtained with silica gel 60 F254 as the<br />stationary phase, isopropanol:water:25% ammonia (6:3:1) as the mobile phase, and a maximum wavelength of about 260 nm. Validation results<br />indicated that the accuracy of the analytical method for DSG was about 99.11–99.96% with a coefficient variation (precision) of 0.70–1.41%, while<br />that for DSI was 98.56–101.05% with a coefficient variation of 0.23–0.75%. The correlation coefficients for the analytical method for DSG and DSI<br />were 0.9909 and 0.9976, respectively. The results determined that the levels of DSG and DSI in samples A, B, C, D, E, and F were 0.70%/0.60%,<br />0.79%/0.74%, 0.78%/0.71%, 0.99%/0.66%; 1.08%/0.54%, and 1.08%/0.67%, respectively.</p> ER -