EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF ‘KARIRA’-A VALUABLE AYURVEDIC PLANT

PREETI VERMA; DHARMISHTHA PARMAR; BHANUBHAI SUHAGIA

doi:10.22159/ijcpr.2023v15i6.3077

Authors

PREETI VERMA L. M. College of Pharmacy, Navrangpura, Ahmedabad-380009, Gujarat, India
DHARMISHTHA PARMAR L. M. College of Pharmacy, Navrangpura, Ahmedabad-380009, Gujarat, India
BHANUBHAI SUHAGIA Faculty of Pharmacy, Dharmsinh Desai University, Nadiad-387001, Gujarat, India

DOI:

https://doi.org/10.22159/ijcpr.2023v15i6.3077

Keywords:

Karira, Capparis decidua, Capparidaceae, Hepatoprotective, Carbon tetrachloride, Antioxidant

Abstract

Objective: ‘Karira’ is a common Sanskrit name of an important Ayurvedic medicinal plant, Capparis decidua Edgew. (family, Capparidaceae). It is a bushy shrub or small tree distributed throughout the dry areas of India and other parts of the world. As per traditional literature, various parts of the plant are widely used in the treatment of biliousness, jaundice, andas a liver tonic. Therefore the present study was aimed at the evaluation of hepatoprotective activity of Karira stem.

Methods: For the study, three types of extracts of C. decidua stem were prepared, viz. aqueous extract, alcoholic extract and 50% hydroalcoholic extract. The stem extracts were evaluated for their protective effects against carbon tetrachloride-induced acute hepatotoxicity in the rats in in vivo conditions.

Results: The results suggested that among all the three extracts, specifically the alcoholic extract at the dose of 300 mg/kg exhibited highest hepatoprotective activity, which was comparable to the standard drug, silymarin (50 mg/kg). The hepatoprotective effects were further confirmed by detailed antioxidant study, which revealed significant free radical scavenging activities and protection against lipid peroxidation offered by the extracts, along with preservation of the integrity of liver cells as evident from the histopathological study of the liver tissues.

Conclusion: The present investigation supports the traditional use of Karira plant in liver-related disorders.

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References

Chatterjee TK. Medicinal plants used as antidiabetic agents. Herb Options Calcutta M/S East Traders; 1996.

Plaa GL, Charbonneau M. Detection and evaluation of chemically induced liver injury. In: Wallace HAW, editor. Principles and methods of toxicology. New York: Raven Press; 1994.

Seki S, Kitada T, Sakaguchi H. Clinicopathological significance of oxidative cellular damage in non-alcoholic fatty liver diseases. Hepatol Res. 2005;33(2):132-4. doi: 10.1016/j.hepres.2005.09.020, PMID 16198621.

Evans WC. An overview of drugs with anti-hepatotoxic and oral hypoglycaemic activities. In: Trease, Evans, editors. Pharmacognosy. 15th ed Elsevier: Saunders; 2008.

Kirtikar KR, Basu BD. Indian medicinal plants. Vol. I. 2nd ed. Delhi: M/s Bishen Singh Mahendra Pal; 1975.

Nadkarni KM. Indian materia medica-vegetable kingdom. 3rd ed. Vol. I. Mumbai: Popular Press Book Depot; 1954.

Abhinavanighatu CD. Mathura: Bombay Bhushan Press; 1986.

Shashtri GM. Bhavaprakash purvakhand. 1st ed. Mumbai: Sastu Sahitya Vardhak Karyalaya; 1958.

Sharma PV. Dravyaguna vigyana. 2nd ed. Vol. III. Varanasi: Chaukhambha Bharti Academy Publisher; 2005.

Marwat SK, Rehman F, Usman K, Khakwani AA, Ghulam S, Anwar N. Medico-ethnobotanical studies of edible wild fruit plant species from the flora of North-Western Pakistan (D.I. Khan District). J Med Plants Res. 2011;5(16):3679-86.

Masoodi MH, Khan SA, Khan S, Verma A, Ahmed B. Evaluation of anti-hepatotoxic activity of Lychnis coronaria L. aqueous extract in carbon tetrachloride-induced toxicity. Indian Drugs. 2007;44(8):618-21.

Mani Senthilkumar KTM, Rajkapoor B, Kavimani S. Protective effect of Enicostemma littorale against CCl4¬−-induced hepatic damage in rats. Pharm Biol. 2005;43(5):485-7. doi: 10.1080/13880200590963952.

Teitz NW. SGPT kinetic UV test optimized by expert panel on enzyme of the IFCC. Clin Chim Acta. 1976;70.

Young DS, Tietz N. Kinetic UV optimized IFCC method. Fund Clin Chem. 1973;10:58.

Metcalf MG. Rapid gas chromatographic assay for pregnanolone in pregnancy urine. Clin Chem. 1969;15(1):24-30. doi: 10.1093/clinchem/15.1.24, PMID 5762675.

Michealson SR, Gambino M. Estimation of total and direct bilirubin. In: Tietz NW, editor. Fundamentals of clinical chemistry. Philadelphia: B. Saunders Company; 1986.

Tietz NW, Young DS, Natio NK. Colorimetric method for estimation of cholesterol. Fund Clin Chem. 1973;10:79.

Gournall A. Colourimetric method for estimation of total protein. J Biol Chem. 1949;751.

Gindler EM, Westgard JO G. Colourimetric method. Clin Chem. 1973;6:4.

Siemens AJ, Kalant H, Khanna JM, Marshman J, Ho G. Effect of cannabis on pentobarbital-induced sleeping time and pentobarbital metabolism in the rat. Biochem Pharmacol. 1974;23(3):477-88. doi: 10.1016/0006-2952(74)90612-1, PMID 4822738.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75. doi: 10.1016/S0021-9258(19)52451-6, PMID 14907713.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8. doi: 10.1016/0003-2697(79)90738-3, PMID 36810.

Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta. 1979;582(1):67-78. doi: 10.1016/0304-4165(79)90289-7, PMID 760819.

Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972;247(10):3170-5. doi: 10.1016/S0021-9258(19)45228-9, PMID 4623845.

Aeibi H, Bergmeyer H. Methods in enzymatic analysis. 2nd ed. New York: Academic Press; 1974.

Prophet EB, Mills B, Arrington JB, Sobin LH. Laboratory methods in histotechnology. Washington, DC: Armed Forces Institute of Pathology; 1992.

Kulkarni SK. New Delhi: Vallabh Prakashan. Handb Exp Pharmacol. 1993.