Innovare Journal of Life Sciences

ADVANTAGEOUS OF BENEFICIAL MICROBES FOR BIOREMEDIATION OF ADULTERATED GLOBAL-CULTIVATED SOILS

2023-05-31T15:57:21+0530

The soil is one of the most valuable resources since it forms the foundation for many important life processes and ecosystem purposes. Worldwide, soil pollution is a result of human activities that are not sustainable, such as the use of dangerous inorganic chemicals. The mining, production, transportation, end-user use, disposal, and accidental discharge of chemicals all contribute to soil contamination, which in turn jeopardizes human life, livestock, wildlife, and entire ecosystems. Purifying and decontaminating soil with conventional procedures is labor-intensive and time-consuming and can modify the soil’s physical, chemical, and biological properties. Furthermore, they do not always ensure that all impurities are eliminated. Sustainable and cutting-edge technology has developed over the last few decades. Biological soil remediation solutions, also known as soft remediation options, are being developed to integrate, namely efficient removal of soil contaminants, mitigation of soil ecotoxicity, and reduction of legally and ethically mandated hazards to the environment and human health. Soil remediation methods should not only repair soil health and provide necessary system services but also reduce noxious waste concentrations in the soil to below regulatory limits. The microorganisms have shown promise in the clean-up of soils contaminated with radioactive contaminants, heavy metals, chemical fertilizers in excess, trichlorethylene, trinitrotoluene, herbicides such as atrazine, and organophosphates. The cost of cleaning up environmental pollutants with eco-friendly technology is inexpensive when compared to other approaches, including conventional ones. The focus of the current manuscript is on using beneficial bacteria to clean up polluted farmland to ensure the longevity of the subsequent generation.

ASPECTS OF MORPHOGENETIC TRAITS ASSOCIATED WITH PHYSIOLOGICAL COEFFICIENTS RELATED TO INCIDENCE OF OBESITY, HYPERTENSION, AND DIABETES MELLITUS

2023-05-22T11:59:53+0530

The vulnerability to develop certain pathophysiological conditions may be linked to the morphogenetic traits of individuals. Hence, this study ascertained the relationship between some morphogenetic traits and physiological coefficients related to incidence of obesity, hypertension, and diabetes mellitus. Morphogenetic traits (height, weight, ABO blood group, Hitchhiker thumb, tongue rolling, and hand clasping) and physiological coefficients; body mass index, blood pressure (BP), random blood sugar (RBS), and fasting blood sugar (FBS) were measured during a free medical outreach in an institution in Eastern Nigeria. Seventy individuals participated; age (15–35 years), females: 36 (51.4%), males: 34 (48.6%), pre-obese (28.6%), obese 1 and 2 (2.9%, 2.9%), and BP (52.9% normal and 34.3% hypotensive). Twenty-five (35.7%) assayed FBS showed 1.4% diabetes, 17.1% pre-diabetes, and 17.1% normal, while 45 (64.3%) assayed RBS showed 57.1% normal and 7.2% pre-diabetes. There was significantly increased incidence of pre-diabetes and diabetes among tongue rollers when compared to non-tongue rollers (p<0.05). Incidence of obesity and hypertension was not significantly associated with tongue rolling. There was significant association between weight and incidence of obesity (p<0.05); no significant association with weight and incidence of hypertension and diabetes. There was also no association between height, ABO blood group, Hitchhiker thumb, hand clasping and incidence of obesity, hypertension, and diabetes (p<0.05). Conclusively, morphogenetic traits such as tongue rolling and body weight were associated with physiological coefficients related to incidence of diabetes and obesity, respectively, supporting the hypothesis that certain morphogenetic traits can be linked to the proneness of individuals to obesity and diabetes.

EFFECT OF SUBMERGED FERMENTATION ON THE ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AQUEOUS EXTRACTS OBTAINED FROM VOACANGA AFRICANA STAPF SEED

2023-06-08T15:25:50+0530

Voacanga africana Stapf which belongs to the family, Apocynaceae is among useful medicinal plants of West Africa. The present study investigates the antibacterial and antioxidant properties of aqueous extracts obtained from raw and fermented V. africana. One portion of V. africana was subjected to submerged fermentation for 7 days. Aqueous extracts of the raw and fermented samples of V. africana were obtained using standard methods. Antibacterial effect of the aqueous exracts was assessed by agar well diffusion while a battery of antioxidant test which include DPPH scavenging, ABTS, and Fe2+ chelation was employed. The bioactive compounds present in the extract were assessed using gas chromatography-mass spectrophotometer. Extract obtained from the fermented seed of V. africana had good antimicrobial effect with zones of inhibition ranging from 4.67 to 22.00 mm. Aqueous extract obtained from fermented V. africana exhibited better antioxidant capacity with DPPH scavenging effect being the highest (27.93) when compared with extract obtained from raw V. africana. Bioactive compounds such as Eicosane, 1,14-Dibromotetradecane, 7-Oxodehydroabietic acid, oleic acid and so on were present in the aqueous extract obtained from fermented V. africana. Data gathered from this study revealed that fermentation enhances the antimicrobial and anoxidant properties of V. africana. The presence of bioactive compounds in the aqueous extract of V. africana indicates that it could be a good source of natural antimicrobial and antioxidant compounds for the improvement of human health.

ACTIVITY OF LIVER FUNCTION ENZYME AND ANTIBACTERIAL SUSCEPTIBILITY OF ANNONA SENEGALENSIS LEAF EXTRACT

2023-06-02T13:39:16+0530

In ethnomedicine, various natural products have been implicated in the treatment of several diseases. Annona senegalensis has been identified as one of the plants that have the potential to cure ailments arising from microbial infections. This study however was carried out to investigate the liver function enzyme activity and in vitro antibacterial susceptibility of the 50% ethanol-methanol leaf extract of A. senegalensis. The effect of the administration of 100 mg/kg body weight of A. senegalensis on serum enzyme parameters and antibacterial susceptibility was investigated in albino rats. Thirty-three albino rats with average weights of 200 g were divided into two groups. Group 1 contained 30 rats and was treated with 20 mg/mL of A. senegalensis leaf extract while Group 2 contained three rats and served as the control. The treatment lasted for 20 consecutive days while the rats in Group 1 were daily administered with the leaf extract of A. senegalensis. Data were analyzed and presented using a descriptive analysis from Microsoft Excel 2016 version. The results obtained from administration of the leaf extract of A. senegalensis on serum enzymes parameters show a significant increase in serum enzyme activity when compared to the control. This indicates that the leaf extracts of A. senegalensis have significant effects on the serum enzymes parameters. The in vitro antibacterial susceptibility of the leaf extracts relative to the drugs (Ampicillin and Ciprofloxacin) revealed that the leaf extract of the plant holds much promise in antibacterial property when combined with antibiotics used in this study. This research revealed that there is high activity of alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase in the serum following the administration of 100 mg/kg body weight of 50% ethanol-methanol leaf extract of A. senegalensis. The antibacterial susceptibility test of the leaf extract carried out in vitro also revealed the plant antibacterial property. The combination of A. senegalensis leaf extract with antibiotic drugs, especially the one used in this study hold much promise in its efficacy as revealed in its synergistic interaction.

ASSESSMENT OF ANGIOSPERMIC FLORA AT RAJSHAHI METROPOLITAN AREA OF BANGLADESH

2023-05-24T10:45:38+0530

Objectives: The study aimed to document the angiospermic flora at Rajshahi metropolitan area of Bangladesh. The study also examined the species diversity in the study area.

Methods: The research work is based on fresh materials collected during 33 field visits at Rajshahi metropolitan area of Bangladesh was carried out from February 2016 to March 2017 to cover the seasonal variations. Plant parts with either flower or fruits collected using traditional herbarium techniques to make voucher specimens for documentation.

Results: The results show that a total of 494 species belonging to 455 genera and 106 families were recorded. Of these, Magnoliopsida (Dicotyledones) is represented by 413 species under 390 genera and 84 families while Liliopsida (Monocotyledones) is represented by 81 species under 65 genera and 22 families. For each species scientific name, local name, family name, habit, relative occurrence, and flowering time were recorded. The present study will help in identifying the major angiospermic plant species for further investigation.

Conclusion: The present research focused on the angiospermic flora growing throughout the Rajshahi metropolitan area of Bangladesh. A total of 494 angiospermic plant species were recorded.

OPTIMIZATION OF COLONY POLYMERASE CHAIN REACTION FOR THE 16SRRNA OF DIFFERENT STRAINS OF ESCHERICHIA COLI

2023-04-30T00:36:29+0530

Objective: This work aimed to enhance colony polymerase chain reaction (PCR) for the 16S rRNA of several Escherichia coli strains.

Methods: The isolation of E. coli is done from the gut of the chicken and soil. Then, we optimized the condition for colony PCR for the amplification of 16s ribosomal RNA. We successfully designed primer 3 for 16s ribosomal RNA and made the dilution solution with PCR grade water that is 1:10. Moreover, finally, we made a 20 μL solution that contains the master mix of our isolated colony and forward and reverse base primer for amplification. After the conventional PCR, the amplified 16s ribosomal RNA was then run on Gel to obtain the desired bands. And finally saw the bands in the Gel Doc picture.

Results: Our result shows that the technique of colony PCR is an efficient and quick method than other existing methods that are too costly, tedious, and time-consuming procedures that deter their exploitation in various experimentations and for the identification of E. coli strains.

Conclusion: This study concluded that 16s ribosomal RNA can be amplified without the extraction and purification of total genomic DNA from a bacterial colony using colony PCR. Therefore, by designing rRNA primers for E. coli species, we can evaluate their various types of mutations, strain detection, and antibiotic resistance.

BIODEGRADATION OF GLYPHOSATE CONTAINING HERBICIDE BY SOIL FUNGI

2023-07-17T04:08:49+0530

Ten fungal isolates were isolated from two herbicide-contaminated soil farms obtained from Amoyo and the University of Ilorin environment in Kwara State after enrichment with mineral salt medium (MSM) supplemented with glyphosate-containing herbicide. The growth of fungal isolates was efficiently stimulated by the organophosphorus herbicide. Fungi isolated were subjected to screening by varying the herbicide concentrations from 0.1 to 3%, which is prepared with MSM. This screening showed that all the fungal isolates had the ability to act in the biodegradation process. However, varying degradative potentials were observed, as some had heavy growth while others had only slight or no growth as the concentrations of the herbicide increased. The ten fungal isolates were characterized and identified as Aspergillus niger, Penicillum spinulosum, Aspergillus terrus, Aspergillus flavus, Fusarium oxysporum, Mucor spp., Aspergillus oryzae, Aspergillus tamari, Rhizopus stolonifer, and Trichoderma koningii, and these were reduced to six after screening with 3% concentration of the herbicide. Four isolates (A. niger, F. oxysporum, Mucor spp., and A. flavus) were selected based on their growth ability on the medium during screening and were used in the biodegradation study. However, there is an increase in fungal dry weights ranging from 8.60 to 18% for 12 days. This shows that these fungi can be employed in the biodegradation of herbicides since they are potentially effective species and are environmentally safer alternative to protect the soil from the contamination of glyphosate-containing herbicide residues.

CHARACTERIZATION AND MOLECULAR DOCKING OF CINNAMIC ACID DERIVATIVES: POTENTIAL INHIBITORS OF CYCLOOXYGENASE ENZYMES

2023-09-26T23:35:25+0530

Objective: The pathology of every disease passes through the inflammation stage; hence, the design and optimization of potential lead compounds as anti-inflammatory agents is still a significant part of medicinal chemistry globally.

Methods: In this study, we designed, synthesized, and characterized some cinnamic acid derivatives and performed molecular docking of the derivatives on the human cyclooxygenase-1 (COX-1) enzyme.

Results: The elemental analysis showed the presence of different functional groups. Molecular docking was performed on the active sites of COX-1 (PDB ID: 6Y3C). The derivatives as well as the standard compound, were observed to interact mainly with the arginine residue of the target protein. The dioxomethylene substituted derivative showed the highest binding affinity, compared with other derivatives, including the standard drug (−6.8 kcal/mol).

Conclusion: The binding affinity observed in the cinnamic derivatives, and biological activities correlations revealed that compounds with the dioxomethylene group would be good anti-inflammatory lead molecules, as they demonstrated high affinity to the target protein and biological activities. Thus, these compounds can serve as potential lead compounds for the design, and development of effective anti-inflammatory agents, targeted to inhibit the human COX-1 enzyme involved in biological inflammatory mechanisms.