Int J Pharm Pharm Sci, Vol 9, Issue 11, 253-254Erratum

HYPERHOMOCYSTEINEMIA LEAD TO TRANSMURAL INFLAMMATION OF COLON AND INCREASE SEVERITY OF DISEASE IN ACETIC ACID-INDUCED COLITIS IN RAT

LAMDA SOUAD^1*, NAIMI DALILA¹, AGGOUN CHERIFA²

¹Ecole Nationale Superieure de Biotechnologie (ENSB), Nouveau Pôle Universitaire Ali Mendjeli, BP E66, 25100 Constantine, Algeria, ²Animal Biology Departement, Faculty of Life Science and Nature, Frères Mentouri University, Constantine, Algeria
Email: souadlamda@gmail.com

Ref: http://www.ijppsjournal.com/Vol9Issue6/16036.pdf

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ABSTRACT

Objective: The present study was designed to evaluate the effect of hyperhomocysteinemia (Hhcy) induced by feeding rats high methionine diet on the colon wall. Colonic damages caused by Hhcy were compared with those induced by acetic –acid induced colitis.

Methods: Sprague-Dawley rats (200-250g) were divided into four groups: group C (control), group M (received 1 g/kg methionine p. o. during 15 d), group A (colitis was induced by transrectal administration of acetic acid 4% on 8th day) and group MA (received methionine and acetic acid). At the end of the study, plasma homocysteine, C-reactive protein (CRP) and leukocytes (WBC) count were evaluated, all rats were sacrificed and distal 8 cm of the colon was dissected. Colon was weighed for disease activity index (DAI) and injuries were assessed macroscopically and histologically.

Results: High methionine diet induced significant (P<0.001) increase of homocysteine (hcy), CRP levels and WBC count compared to control. Acetic acid rats showed a significant decrease of WBC count. Mixed treatment caused a significant increase of hcy, CRP and a significant decrease of WBC count. Our results showed that Hhcy causes significant damages and immune cells infiltration in all layers of the colonic wall.

Conclusion: The present investigation demonstrated that Hhcy increased the major inflammatory markers as CRP and leukocytes count and produced transmural colitis in rats. Effect of Hhcy is more toxic on the colonic wall than acetic acid indeed while acetic acid lesions are localized in mucosa and submucosa the lesions of hcy extend to the all layers (mucosa, submucosa and muscularis propria). Acetic acid induced colitis in hyperhomocysteinemic rats increased the severity of colitis.

Keywords: Methionine, Hyperhomocysteinemia, Acetic acid, Inflammation, Colonic wall

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Section Page No.	Line No.	Column 1/2	Uncorrected text	Corrected text
Abstract p108	8		Mixed treatment caused a significant increase of hcy, CRP and a significant decrease of WBC count.	Mixed treatment caused a significant increase of hcy, CRP and WBC count
Results Effects of high methionine diet and acetic acid on biochemical and hematological parameters p 109	2	2	Methionine supplementation was found to increase plasma levels of hcy, and CRP, in all treated groups (M and MA) compared to control group (p˂0.0001)	Methionine supplementation was found to increase plasma levels of hcy, CRP and WBC count in all treated groups (M and MA) compared to control group (p˂0.0001).
Results Effects of high methionine diet and acetic acid on biochemical and hematological parameters P 109	5	2	Compared to the acetic acid group (A), methionine-induced significant increase of hcy (p˂0.001) and CRP (p˂0.001) levels in MA group.	Compared to the acetic acid group (A), methionine-induced significant increase of hcy, CRP levels and WBC count (p<0.001) in M and MA groups.
Results Effects of high methionine diet and acetic acid on biochemical and hematological parameters P 109	6	2	Results showed no significant increase of hcy and CRP levels in MA when compared to M.	Results showed significant increase of WBC levels and no significant increase of hcy and CRP levels in MA when compared to M.
Results Effects of high methionine diet and acetic acid on biochemical and hematological parameters P 109	7	2	Acid acetic induced no significant increase of hcy and CRP levels compared to control (table 4).	Acetic acid group induced significant decrease of WBC count (p<0.001) and no significant increase of hcy (p=0.448) and CRP (p=0.081) levels compared to control.
Results Effects of high methionine diet and acetic acid on biochemical and hematological parameters P 109	8-14	2	All groups showed significant changes of WBC count (p˂0.001) when compared to control. While methionine increased WBC count in M group, acetic acid (A) and mixed treatment (methionine and acid acetic: MA) induced a decrease of WBC count (p˂0.001). Results of MA group showed a significant decrease of WBC compared to acetic acid control (A) and methionine control (M) (table 4)	The associated treatments methionine-acetic acid showed significant increase of hcy, CRP and WBC count (p<0.001) compared to control and A groups. (table 4)
Results P 110 Table 4: Effects of high methionine diet and acetic acid on biochemical and hematological parameters hcy CRP WBC C 8.05±0.72 0.02±0.01 12.21±0.30 M 47.20±2.89*** 0.31±0.03*** 15.35±0.45*** A 11.53±1.24 0.09±0.01 8.19±0.36*** MA 50.6±4.83***ϒϒϒ 0.33±0.07***ϒϒϒ 13.71±0.59***ϒ ϒϒ◊◊◊ ANOVA F 193.893 66.549 194.855 Df 3.098 3.098 3.098 P <0.00000 <0.00000 <0.00000 Data are expressed as mean±SEM of body weight change (%) (n=6), *p˂0.05, **p˂0.01, ***p˂0.001 when compared to C, ϒ p˂0.05, ϒϒp˂0.01, ϒϒϒp˂0.001 when compared to A, ◊p˂0.05, ◊◊p˂0.01, ◊◊◊p˂0.001 when compared to Section page No. Line n ° Column 1/2 Uncorrected text Corrected text Discussion P 114 13 1 significant increase of WBC count significant decrease of WBC count Discussion P 114 4 2 there was a significant decrease of WBC count there was a significant increase of WBC count