EFFECT OF BLUMEA LACERA ON TISSUE GSH, LIPID PEROXIDATION AND HEPATIC CELLS IN ETHANOL INDUCED HEPATOTOXICITY IN RATS
DOI:
https://doi.org/10.22159/ijpps.2019v11i12.36453Keywords:
Blumea lacera, Hepatoprotective, In vivo antioxidant, EthanolAbstract
Objective: To evaluate hepatoprotective effects of ethanol extract of aerial part of Blumea lacera (BLEE) against ethanol-induced hepatotoxicity in rats.
Methods: The in vivo antioxidant activity of BLEE was assessed by determining the tissue glutathione (GSH) and lipid peroxidation (LPO) levels. The BLEE at the doses of 200 and 400 mg/kg and silymarin 100 mg/kg administered to the ethanol challenged rats. The effects of BLEE and silymarin on Physical and Biochemical Parameters were measured. Similarly, histopathological changes of the liver were studied.
Results: The BLEE showed in vivo antioxidant activity. A significant (P<0.001) decrease in SGOT, SGPT, ALP, total and direct bilirubin was observed in BLEE treated group at doses i.e. 200 mg/kg and 400 mg/kg as compared to intoxicated group. Liver damage in animal pretreated with BLEE was minimal with distinct preservation of structures and the architectural frame of the hepatic cells.
Conclusion: These findings demonstrated the hepatoprotective effects of BLEE against ethanol-induced liver damage.
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References
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2. Saraswat B, Visen PKS, Patnaik GK, Dhawan BN. Ex vivo and in vivo investigation of picroliv from Picorrhiza kurroa in an alcohol intoxication model in rats. J Ethanopharmacol 1999;66:263-9.
3. Saravaran N, Nalini N. Antioxidant effect of Hemidesmus indicus on ethanol-induced hepatotoxicity in rats. J Med Food 2007;10:675-82.
4. Modi H, Patel V, Patel K. Hepatoprotective activity of aegle marrmelos against ethanol-induced hepatotoxicity in rats. Asian J Pharm Clin Res 2012;5:164-7.
5. Ansari JA, Sayyed M, Sayyed F. management of non-alcoholic fatty liver disease and their complications. Int J Pharmacol 2011;7:579-88.
6. Varier PSV. Indian medicinal plants. Arya Vaidyasala Kottakkal, Orient Longman; 1995. p. 278.
7. Kirtikar KR, Basu BD. Indian medicinal plants. International Book distribution, Dehradun; 2003. p. 1340.
8. Singh B, Singh S, Singh Surendra, Ali L, Mahajan R. Ethanobotanical significance and antimicrobial activity of Blumea lacera. IJPBA 2010;1:314-6.
9. Rastogi RP, Mehrotra BN. Compendium of Indian medicinal plants, CDRI Lucknow and NISC New Delhi; 1999. p. 102.
10. Veeraraghavan P. Expert consultant, CPCSEA, OECD guideline No. 420; 2000.
11. Gulati RK, Agarwal S, Agarwal SS. Hepatoprotective studies on phyllanthus emblica linn and quercetin. Ind J Exp Biol 1995;33:261-8.
12. Kapoor V, Pillai K, Hussain SZ, Balani DK. Hepatoprotective activity of “jigrine” on liver damage caused by alcohol, carbon tetrachloride and paracetamol in rats. Indian J Pharmacol 1994;26:35-40.
13. Sharma A, Sangameswaran B, Jain V, Saluja MS. Hepatoprotective activity of Adina cordifolia against ethanol-induced hepatotoxicity in rats. Int Curr Pharma J 2012;1:279-84.
14. Bradley DW, Maynard JE, Emery G, Webster H. Transminase activity in serum of long term hemolysis patients. Clin Chem 2003;18:1442.
15. Rej R, Fasce CF, Vanderlinde RE. Increased aspartase aminotransferase activity of serum after in vitro supplymentation with pyridoxal phosphate. Clin Chem 1973;19:92.
16. McComb RB, Bowers GN Jr. Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum. Clin Chem 1972;18:97.
17. Pearlman PC, Lee RT. Detection of measurement of total Bilirubin in serum with use of surfactants as solubilizing agents. Clin Chem 1974;20:447.
18. Aykae G, Vysal M, Yalein AS, Kocak TN, Sivas A, Oz H. The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats. Toxicology 1985;36:71-6.
19. John Buege A, Steven Aust D. Microsomal lipid peroxidation. London: Moury Kleiman Co; 1978. p. 302.
20. Thurman RG. Alcoholic liver injury involves activation of Kupffer cells by endotoxin. Am J Physiol 1998;275:605-11.
21. Tsukamoto H, Takei Y, McClain CJ, Shimizu H, Sato N, Thurman R. How is the liver primed or sensitized for alcoholic liver disease. Alcohol Clin Exp Res 2001;25:171-81.
22. Zhou Z, Wang L, Song Z, Lambert JC, McClain CJ, Kang YJ. A critical involvement of oxidative stress in acute alcohol-induced hepatic TNF-alpha production. Am J Pathol 2003;163:1137-46.
23. Kennedy NY, Tipton KF. Ethanol metabolism and alcoholic liver disease. Essays Biochem 1990;25:137-95.
24. Albano E, Towasi A, Goria GL, Diazani MU. Spin trapping of free radical species produced during the microsomal metabolism of ethanol. Chem Biol Interact 1988;65:223-34.
25. Goldberg DM, Watts C. Serum enzymes changes as evidence of liver reaction to oral alcohol. Gastroenterology 1965;49:256-61.
Published
01-12-2019
How to Cite
SHIRODE, D. S., A. V. KULKARNI, and B. B. JAIN. “EFFECT OF BLUMEA LACERA ON TISSUE GSH, LIPID PEROXIDATION AND HEPATIC CELLS IN ETHANOL INDUCED HEPATOTOXICITY IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 11, no. 12, Dec. 2019, pp. 46-50, doi:10.22159/ijpps.2019v11i12.36453.
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