EVALUATION OF SINIGRIN EFFECT IN NEUROPROTECTION AGAINST PARKINSON’S DISEASE AND NEUROPATHIC PAIN

RACHANA SARAWADE; C. D. UPASANI

doi:10.22159/ijpps.2025v17i1.52699

Authors

RACHANA SARAWADE Department of Pharmacology, Dr. L. H. Hiranandani College of Pharmacy, Ulhasnagar, Mumbai, India https://orcid.org/0009-0003-0805-6789
C. D. UPASANI Pharmacology Department, SNJB’s SSDJ College of Pharmacy, Chandwad, Nashik, Maharashtra, India https://orcid.org/0000-0003-4446-1557

DOI:

https://doi.org/10.22159/ijpps.2025v17i1.52699

Keywords:

Sinigrin, Neuroprotective, Excitotoxicity, Parkinson’s disease (PD), Neuropathic pain (NP), N-methyl-D-aspartate receptor (NMDARs) antagonism, Zero order method, First order derivative method, RP-HPLC, Validation, Bupropion, Dextromethorphan., Pregabaline

Abstract

Objective: The present study aims to evaluate the neuroprotective activity of Saponin: Sinigrin against Parkinson’s Disease (PD) and associated neuropathic pain in rat model. A correlation between Parkinson’s Disease (PD) associated neuropathic pain and predicting antioxidant, neuroprotective effects of Saponin: Sinigrin and its interspecific relation with the underlying mechanism.

Methods: Excitotoxicity with Mono Sodium Glutamate (MSG) (2gm/kg i.p) and neurotoxicity with Acrylamide (ACR) (30mg/kg,i.p) was induced in rats, treated with standard dextromethorphan (30mg/kg p.o), and Pregabalin (10mg/kg,po) and test compound (Sinigrin 75 mg/kg) were tested for behavioral parameters viz: muscle rigidity, locomotor activity, mechanical hyperalgesia, cold allodynia, etc and biochemical estimation from brain and sciatic nerve homogenate by sacrificing animals was done. Estimation of brain neurotransmitters (Dopamine, Gamma-Amino Butyric Acid (GABA) antioxidants, Glutathione (GSH) and Catalase(CAT), and oxidative stress Super Oxide Dismutase (SOD), Nitric oxide (NO) concentration, Thiobarbituric Acid Reactive Substances (TBARS) and Myloperoxidase activity(MPO) was done. Statistical analysis was done using Analysis of Variance (ANOVA) followed by Tukey’s multiple comparison tests.

Results: Sinigrin showed a significant neuroprotective activity in rats compared to monosodium glutamate (2gm/kg i.p. It was observed from the study that test drug Sinigrin produced a significant (p≤0.05) reduction in muscle rigidity, increased locomotor activity, left hind paw lifting duration, improved cold allodynia, and thermal hyperalgesia. Brain neurotransmitter levels, antioxidant (p≤0.01) were increased, and oxidative stress (p≤0.01) was also reduced to that of the standard drug dextromethorphan.

Conclusion: The study suggests that Sinigrin is neuroprotective and can be used in the treatment of Parkinson’s Disease (PD) and associated Neuropathic Pain (NP).

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