TY - JOUR AU - K., Bashir M. AU - S., Ismail AU - K., Lam C. AU - K., Chua J. AU - H., Hussin A. PY - 2015/11/01 Y2 - 2024/03/29 TI - THE IN VITRO AND EX VIVO EFFECT OF PHYLLANTHUS NIRURI METHANOL EXTRACT ON HEPATIC UDP-GLUCURONYLTRANSFERASE ENZYME ACTIVITY IN STZ-INDUCED DIABETIC SPRAGUE DAWLEY RATS JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 7 IS - 11 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/7049 SP - 105-109 AB - <p><strong>Objective:&nbsp;</strong>The aim of the study was to investigate the&nbsp;<em>in vitro</em>&nbsp;and&nbsp;<em>ex vivo</em>&nbsp;(acute and sub-chronic doses) effect of&nbsp;<em>Phyllanthus niruri</em>&nbsp;methanol extract (PNME) on the microsomal UDP-glucuronyltransferase (UGT) enzyme activity in streptozotocin (STZ)-induced diabetic young female Sprague Dawley (SD) rats.</p><p><strong>Methods:&nbsp;</strong>Young female SD rats were induced type I diabetes mellitus using STZ (60 mg/kg i. v.). The&nbsp;<em>in vitro</em>&nbsp;study was performed on a microsomal fraction of diabetic rat livers using PNME in doses of (0.01 µg, 1 µg and 10 µg)/ml. While&nbsp;<em>ex vivo</em>&nbsp;studies were performed on the microsomal fraction of diabetic rats using PNME in doses of 500, 1000, 2000 and 5000 mg/kg p. o. for acute&nbsp;<em>ex vivo</em>&nbsp;study (one-day treatment) and 100, 500 and 2000 mg/kg/day p. o. for sub-chronic one (daily dose for two weeks).&nbsp;<em>p</em>-nitrophenol (<em>p</em>-NP), was used as a marker substrate for UGT enzyme activity and analyzed using the spectrophotometer to determine UGT enzyme activity.</p><p><strong>Results:&nbsp;</strong>The&nbsp;<em>in vitro</em>&nbsp;result showed that, there is no significant effect of the three concentrations of PNME&nbsp;<em>versus</em>&nbsp;control on UGT activity in the microsomal fraction of diabetic young female SD rat livers, while for&nbsp;<em>ex vivo</em>&nbsp;study, the result showed that UGT activity in the microsomal fraction of diabetic young female SD rats significantly and dose-independently increased at doses 1000, 2000 and 5000 mg/kg p. o in acute study (all&nbsp;<em>p</em>&lt;0.05&nbsp;<em>vs</em>&nbsp;control). However, no significant effect of PNME has been seen in the three doses used in the sub-chronic study.</p><p><strong>Conclusion:&nbsp;</strong>The three different concentrations of PNME have no significant effect as compared to control on UGT activity in the&nbsp;<em>in vitro</em>&nbsp;study. However,&nbsp;<em>ex vivo</em>&nbsp;study showed significant and dose-independent increased in UGT activity at doses 1000, 2000, and 5000 mg/kg p. o in acute study (all P&lt;0.01 vs control), but no significant effect on sub-chronic study.</p> ER -