EVALUATION OF THE FOLK CLAIM AND IDENTIFICATION OF THE PHARMACOLOGICAL ACTIVE PRINCIPLES IN BAUHINIA PHOENICEA LEAVES
Abstract
ABSTRACT
Objective: Bauhinia phoenicea, an endemic plant of western Ghats, using in traditional medicine against diabetes, skin allergies, fungal infections, and
worm disturbances. To the best of our knowledge, any scientific studies on the medicinal properties of the leaves of this plant are not yet reported.
Therefore, as the first step toward unraveling its medicinal property, bioactivity profiling, and gas chromatography-mass spectrometry (GC-MS)
analysis were performed.
Methods: The pharmacological activity profiling includes antibacterial, antifungal, anthelmintic, and antioxidant property screening using crude
ethanolic extract, which preliminary analyses its folk claim. GC-MS analysis performed to identify the pharmacologically active principles. All analyses
were performed according to standard protocols.
Results: Ethanol extract of the leaves of B. phoenicea was assayed for the antimicrobial activity against 10 human pathogenic strains using well
diffusion assay. The extract showed a significant activity against all pathogens. A maximum zone of inhibition observed in Salmonella typhi in their
higher concentration (500 µg/ml). The anthelmintic activity of crude drug evaluated on Indian adult earthworms Pheretima posthuma, exhibited dosedependent
spontaneous mortality,
and evoked
responses
to
pin prick
and effects
compared
with that of albendazole. The drug showed
potent
antioxidant
property
in 2,2-diphenyl-1-picrylhydrazyl
free
radical
scavenging
and superoxide
anion scavenging
assays
with half maximal inhibitory
concentration
values
92±0.92 and 62±1.34, respectively.
The GC-MS
analysis
which
showed
the presence
of 19 compounds including hexadecanoic
acid and oleic acid.
Conclusion: According to our results, it is concluded that leaf of B. phoenicea has significant antimicrobial, anthelmintic, and antioxidant properties
supporting the folk medicinal use of this species. The further procedures of isolation and characterization of active principles are in progress.
Keywords: Bauhinia phoenicea, Antimicrobial, Anthelmintic, Antioxidant property and gas chromatography-mass spectrometry.
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