IN VITRO ANTIOXIDANT ACTIVITY OF DIFFERENT SUBSTITUTED BENZILIC ACID USING 2,2-DIPHENYL-1-PICRYL HYDRAZYL RADICAL, ABTS ASSAY METHOD
Abstract
Objectives: The aim of this study is to investigate the antioxidant activity of different substituted benzilic acid using DPPH assay and ABTS methods.
Methods: Compounds were synthesized based on benzil-benzilic acid rearrangement. The synthesized compound was confirmed by 1HNMR, Infrared,
Mass, TGA, and UV analysis. Antioxidant activity of the compound was measured by radical scavenging assay method (2,2-diphenyl-1-picrylhydrazyl)
and ABTS method.
Results: All synthesized compounds show moderate to extent antioxidant activity. The results for the antioxidant activity showed that the highest
percentage of scavenger radicals was present in the 2'-chloro-4-methoxy-3-nitro benzilic acid.
Conclusion: The result showed that better activity in 2'-chloro-4-methoxy-3-nitro benzilic acid due to the presence of electro withdrawing and
electro donating group than others. Thus, it could be served as potent antioxidant. All compounds were tested for their effect on the viability of cells
and results demonstrated that they are not toxic towards the cell lines used.
Keywords: Synthesis, Antioxidant activity, 2, 2-diphenyl-1-picryl hydrazyl assay, ABTS assay.
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REFERENCES
Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants,
and the degenerative diseases of aging. Proc Natl Acad Sci U S A
;90(17):7915-22.
Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. Dietary
antioxidant flavonoids and risk of coronary heart disease: The Zutphen
elderly study. Lancet 1993;342(8878):1007-11.
Temple NJ, Gladwin KK. Fruit, vegetables, and the prevention of
cancer: Research challenges. Nutrition 2003;19(5):467-70.
Peters U, Leitzmann MF, Chatterjee N, Wang Y, Albanes D,
Gelmann EP, et al. Serum lycopene, other carotenoids, and prostate
cancer risk: A nested case-control study in the prostate, lung, colorectal,
and ovarian cancer screening trial. Cancer Epidemiol Biomarkers Prev
;16(5):962-8.
Yen GC, Chen HY. Antioxidant activity of various tea extracts in
relation to their antimut agenicity. J Agric Food Chem 1995;43:27.
Soler-Rivas C, Espin JC, Wichers H. An easy and fast test to compare
total free radical scavenger capacity of foodstuffs. J Phytochem Anal
;11(5): 330-8.
Sudha R, Kanakam CC, Nithya G. Synthesis characteraisation and
antimicrobial activity of substituted benzilic acids. Int J Chem Tech Res
;8:383-7.
Nithya G, Kanakam CC, Sudha R. Synthesis, characterization, study of
biological activity and molecular docking of benzil and its substituted
analogs. Int J Chem Tech Res 2015;6:241-9.
Shahidi F, Wanasundara PK. Phenolic antioxidants. Cri Rev Food Sci
Nutr 1992;32(1):67.
Sanchez-Moreno C, Larraui JA, Saure-Calixio FJ. A procedure to
measure the antiradical efficiency of polyphenols. Sci Food Agric
;76:270.
Brand-Williams W, Cuvelier ME, Berest C. Use of a free radical method
to evaluate antioxidant activity. Lebenson Wiss Technol 1995;28:25.
Ndhlala AR, Moyo M, Van Staden J. Natural antioxidants: Fascinating
or mythical biomolecules? Molecules 2010;15(10):6905-30.
Roginsky V, Lissi EA. Review of methods to determine chain breaking
antioxidant activity in food. Food Chem 2005;92:235-54.
Lachman L, Lieberman HA, Kanig JL. The theory and practice of
Asian J Pharm Clin Res, Vol 9, Issue 3, 2016, 127-130
Sudha et al.
industrial Momordica charantia L. (bitter gourd). Biosci Biotechnol
Biochem 2003;67:2512-7.
Anto RJ, Sukumaran K, Kuttan G, Rao MN, Subbaraju V, Kuttan R.
Anticancer and antioxidant activity of synthetic chalcones and related
compounds. Cancer Lett 1995;97(1):33-7.
Anto RJ, Kuttan G, Dinesh Babu KV, Rajasekharan KN, Kuttan R. Antitumour
and free radical scavenging
activity
of
synthetic curcuminoids.
Int
J Pharm 1996;131:1-7.
Iverson F. Phenolic antioxidants: Health protection branch studies on
butylated hydroxyanisole. Cancer Lett 1995;93(1):49-54.
Blois MS. Antioxidant determinations by the use of a stable free radical.
Nature 1958;29:1199-200.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C.
Antioxidant activity applying an improved ABTS radical cation
decolorization assay. Free Radic Biol Med 1999;26(9-10):1231-7.
Buenger J, Ackermann H, Jentzsch A, Mehling A, Pfitzner I,
Reiffen KA, et al. An interlaboratory comparison of methods used to
assess antioxidant potentials. Int J Cosmet Sci 2006;28(2):135-46.
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