ANTI-ACNE ACTIVITY OF ACETONE EXTRACT OF PLUMBAGO INDICA ROOT
Abstract
ABSTRACT
Objective: The aim of the present study was to investigate the effect of priming with exogenous sources of calcium ion and nitric oxide on the
antioxidant activity, antidiabetic activity, and related phenolic contents along with the histochemical status of fenugreek sprouts.
Methods: The fenugreek seeds were primed with calcium chloride (CC), calcium chelator ethylene glycol-bis (2-aminoethylether) -N, N, N´, N tetra
acetic acid (EG), sodium nitroprusside (SNP) and 2-(4-carboxyphenyl) -4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (CP) and germinated for 72
hrs. The sprout extracts were investigated for their antioxidant potential by 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis-3-ethylbenzthiazoline-6sulfonic
acid, reducing
power
as well
as
beta-carotene
bleaching assays
along with
in
vitro
antidiabetic
activity by α-amylase and α-glucosidase
inhibition. Along with this, phytochemicals such as phenolics, flavonoids, and carotene content were also estimated, and the histochemical detection
of reactive oxygen species in roots was performed.
Results: The results demonstrated that the seeds pre-treated with CC and SNP showed enhanced antioxidant as well as antidiabetic potential over
control; on the other hand, their action was reversed by their antagonists, EG, and CP. A similar trend was observed in the phytochemical contents of
the sprouts. Furthermore, it was evident from the histochemical detection of H
2
O
and superoxide localization as well as lipid peroxidation and plasma
membrane integrity that the exogenous supply of calcium ion and nitric oxide exhibited protective role in the germinating seedlings.
Conclusions: The study suggests the active involvement of the signal molecules, Ca
2
, and nitric oxide in signaling pathways associated with related
phenolic compounds and oxidative stress management.
2+
Keywords: Antidiabetic, Antioxidant, Calcium ion, Germination, Histochemical localization, Phenolics, Nitric oxide, Trigonella foenum-graecum.
Downloads
References
REFERENCES
Kapoor S, Saraf S. Topical herbal therapies an alternative and
complementary choice to combat acne. Res J Med Plant 2011;5(6):650-69.
Verma H, Prasad SB, Yashwant SH. Herbal drug delivery system:
A modern era prospective. Int J Curr Pharm Rev Res 2013:4(3):88-101.
Azimi H, Fallah-Tafti M, Khakshur AA, Abdollahi M. A review of
phytotherapy of acne vulgaris: Perspective of new pharmacological
treatments. Fitoterapia 2012;83:1306-17.
Khan N, Karodi R, Siddiqui A, Thube S, Rub R. Development of
anti-acne gel formulation of anthraquinones rich fraction from Rubia
cordifolia (Rubiaceae). Int J Appl Res Nat Prod 2012;4(4):28-36.
Yarnell E, Abascal K. Herbal medicine for acne vulgaris. Altern
Complement Ther 2006;12(6):303-9.
Patel RP, Patel KP, Modi KA, Pathak CJ. Novel antiacne drug delivery
system of tretinoin. Int Res J Pharm 2011;1(2):65-71.
Pandey M, Debnath M, Gupta S, Chikara SK. Phytomedicine: An
ancient approach turning into future potential source of therapeutics.
J Pharmacogn Phytother 2011;3(1):113-7.
Leyden JJ. Current issues in antimicrobial therapy for the treatment of
acne. J Eur Acad Dermatol Venereol 2001;15 Suppl 3:51-5.
Jose B, Dhanya BP, Silja PK, Krishnan PN, Satheeshkumar K.
Plumbago rosea L. A review on tissue culture and pharmacological
research. Int J Pharm Sci Rev Res 2014;25(1):246-56.
Indumathy R, Aruna A. Cytotoxic Potential of various extracts of
Lepidium sativum (Linn.). An in-vitro evaluation. Int J Pharmacol
Pharm Sci 2015;2(5):1-5.
Nand P, Drabu S, Gupta RK. Phytochemical and antimicrobial screening
of medicinal plants for the treatment of acne. Indian J Nat Prod Resour
;3(1):28-32.
Lohitha P, Parmesh R. In vitro anti-microbial screening: Mannich
base derivatives of 1,3- di acetyl indole. Int J Pharmacol Pharm Sci
;2(5):33-41.
Lee JH, Lee JS. Chemical composition and antifungal activity of plant
essential oils against Malassezia furfur. Korean J Microbiol Biotechnol
;38(3):315-21.
Athikomkulchai S, Watthanachaiyingcharoen R, Tunvichien S,
Vayumhasuwan P, Karnsomkiet P, Sae-Jong P, et al. The development
of anti-acne products from Eucalyptus globulus and Psidium guajava
oil. J Health Res 2008;22(3):109-13.
Tiwari AK, Mishra RK, Kumar A, Srivastava S, Dikshit A,
Pandey A, et al. A comparative novel method of antifungal susceptibility
for Malassezia furfur and modification of culture medium by adding
lipid supplement. J Phytol 2011;3:44-52.
Chee HY, Lee MH. In vitro activity of celery essential oil against
Malassezia furfur. Mycobiology 2009;37(1):67-8.
Zu Y, Yu H, Liang L, Fu Y, Efferth T, Liu X, et al. Activities of ten
essential oils towards Propionibacterium acnes and PC-3, A-549 and
MCF-7 cancer cells. Molecules 2010;15(5):3200-10.
Kasim LS, Olatunde A, Effedua HI, Adejumo OE, Ekor M, Fajemirokun
TO. Antimicrobial activity of six selected plants against some strains of
pathogenic organisms. J Microbiol Antimicrob 2012;4(3):54-9.
Bhaskar G, Arshia S, Priyadarshini SR. Formulation and evaluation of
topical polyherbal antiacne gels containing Garcinia mangostana and
Aloe vera. Pharmacogn Mag 2009;5:93-9.
Marcon MJ, Durrell DE, Powell DA, Buesching WJ. In vitro activity
of systemic antifungal agents against Malassezia furfur. Antimicrob
Agents Chemother 1987;31(6):951-3.
Crespo MJ, Abarca ML, Cabañes FJ. Isolation of Malassezia furfur
from a cat. J Clin Microbiol 1999;37(5):1573-4.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.