FINGER PRINTING ANALYSIS OF THE ALKALOIDS FROM sphaeranthus amaranthoides LEAVES USING HPTLC ANALYSIS

Abstract

Objective: The present study is conducted to identify the alkaloids from leaf ethanolic extracts of Sphaernathus amaranthoides.

Methods: The leaves extracts was subjected to column chromatography and elutes were collected.   The collected elutes were analyzed using TLC and HPTLC.

Results: The collected elutes were showed the presence of alkaloids in the preliminary analysis, further proved the presence of alkaloids with the TLC and the Rf value is 0.85 and fro HPTLC there are nine different Rf values were identified which are similar to alkaloids.

Conclusion: with the above Rf values we have concluded the presence of alkaloids

In the present study alkaloids were identified from leaf ethanolic extracts of Sphaeranthus amaranthodies. The preliminary phytochemical analysis of the leaf extracts revealed the presence of the alkaloids, flavonoids, phenolics, glucosides, saponins tannins, terpenoids and coumarins etc. The currents results proved the presence of ten different types of alkaloids. The TLC and HPTLC finger printing proved the presence of the ten different alkaloids. 

Key words: phytochemical ; Sphaeranthus amaranthoides leaf extracts; alkaloids;TLC;HPTLC.