ELECTRO-OXIDATION OF NIMESULIDE AT GOLD ELECTRODE AND ITS DETERMINATION IN PHARMACEUTICAL DOSAGE FORM AND HUMAN BIOLOGICAL FLUID
Abstract
ABSTRACT
The electro-oxidation of nimesulide has been investigated by cyclic and differential-pulse voltammetry at different pH at gold electrode. Cyclic voltammetric studies were performed in a wide range of sweep rates and various concentrations of nimesulide. The oxidation process was irreversible and exhibited a diffusion-controlled behavior. According to the linear relation between the peak current and the nimesulide concentration, differential-pulse voltammetric method for the quantitative determination in pharmaceuticals was developed. The recovery was determined to be 97.3 to 100.2 % by means of standard addition method. The linear response was obtained in the range of 2.0 x 10-7 to 1.2 x 10-6 M with a detection limit of 1.11 x 10-9 M with good selectivity and sensitivity. The electrochemical behaviors of nimesulide were studied and electron-transfer coefficient (α = 0.5), proton number (X = 1) and electron transfer number (n = 2) have been determined. The proposed method was also applied for the detection of nimesulide in urine as a real sample.
Keywords: Nimesulide; Gold electrode; Electrochemical behavior; Drug analysisDownloads
References
Bernareggi A. Clinical pharmacokinetics and metabolism of nimesulide. Inflammopharmacology Year: 2001. Volume: 9. Page: 81-89.
Maltese A, Maugeri F, Bucolo C. Rapid determination of nimesulide in rabbit aqueous humor by liquid chromatography. J. Chromatogr. B Year: 2004. Volume: 804. Page: 441-443.
Zacharis CK, Tzanavaras PD, Notou M, Zotou A, Themelis DG. Separation and determination of nimesulide related substances for quality control purposes by micellar electrokinetic chromatography. J. Pharmaceut. Biomed. Anal. Year: 2009. Volume: 49. Page: 201-206.
Wang CY, Shao XQ, Liu QX, Qu QS, Yang GJ, Hu XY. Differential pulse voltammetric determination of nimesulide in pharmaceutical formulation and human serum at glassy carbon electrode modified by cysteic acid/CNTs based on electrochemical oxidation of L-cysteine. J. Pharmaceut. Biomed. Anal. Year: 2006. Volume: 42. Page: 237-244.
Zhang J, Tan X, Zhao D, Tan S, Huang Z, Mi Y, Huang Z. Study of nimesulide and its deter-mination using multiwalled carbon nanotubes modi-fied glassy carbon electrodes. Electrochim. Acta Year: 2010. Volume: 55. Page: 2522-2531.
Hemmateenejad B, Javidnia K, Saeide-Boroujeni M. Spectrophotometric monitoring of nimesulide photodegradation by a combined hard–soft multivariate curve resolution-alternative least square method. J. Pharmaceut. Biomed. Anal. Year: 2008. Volume: 47. Page: 625-630.
Constantinescu IC, Florea M, Arama CC, Nedelcu A, Monciu CM. Assay of nimesulide by ion association titration. Farmacia Year: 2009. Volume: 57. Page: 267-271.
Furlanetto S, Orlandini S, Aldini G, Gotti R, Dreassi E, Pinzauti S. Designing experiments to optimise and validate the adsorptive stripping voltammetric determination of nimesulide. Anal. Chim. Acta Year: 2000. Volume: 413. Page: 229-239.
Catarino RIL, Conceicao ACL, Garcia MBQ, Goncalves MLS, Lima JLFC, dos Santos MMC. Flow amperometric determination of pharmaceuticals with on-line electrode surface renewal. J. Pharmaceut. Biomed. Anal. Year: 2003. Volume: 33. Page: 571-580.
Ferapontova EE. Electrochemistry of guanine and 8-oxoguanine at gold electrodes. Electrochim. Acta Year: 2004. Volume: 49. Page: 1751-1759.
Xia HY, Hu XY. Determination of Isoniazid Using a Gold Electrode by Differential Pulse Voltammetry. Anal. Lett. Year: 2005. Volume: 38. Page: 1405-1414.
Christian GD, Purdy WC. The residual current in orthophosphate medium. J. Electroanal. Chem. Year: 1962. Volume: 3. Page: 363-367.
Malode SJ, Abbar JC, Shetti NP, Nandibewoor ST. Voltammetric oxidation and determination of loop diuretic furosemide at a multi-walled carbon nanotubes paste electrode. Electrochim. Acta Year: 2012. Volume: 60. Page: 95-101.
Barus C, Gros P, Comtat M, Daunes-Marion S, Tarroux R. Electrochemical behaviour of N-acetyl-L-cysteine on gold electrode—A tentative reaction mechanism. Electrochim. Acta Year: 2007. Volume: 52. Page: 7978-7985.
Toghill KE, Compton RG. Electrochemical Non-enzymatic Glucose Sensors: A Perspective and an Evaluation. Int. J. Electrochem. Sci. Year: 2010. Volume: 5. Page: 1246-1301.
Gosser DK. Cyclic Voltammetry: Simulation and Analysis of Reaction Mechanisms, VCH, New York; 1993, p. 43.
Farghaly O, Mohamed N. Voltammetric determination of azithromycin at the carbon paste electrode. Talanta Year: 2004. Volume: 62. Page: 531-538.
Brown ER, Large RF, in: A. Weissberger, B.W. Rossiter (Eds.), Physical Methods of Chemistry, Wiley Interscience. Rochester New York; 1964, p.423.
Laviron E. General expression of the linearpotential sweep voltammogram in the case of
diffusionless electrochemical systems. J. Electroanal. Chem. Year: 1979. Volume: 101. Page: 19-28.
Bard AJ, Faulkner LR. Elecrochemical Methods Fundamentals and Applications, 2nd ed., Wiley, New York; 2004.
Lueje AA, Vasquez P, Vergara LJ N, Squella JA. Voltammetric study of nimesulide and its differential pulse polarographic determination in pharmaceuticals. Electroanalysis Year: 1997. Volume: 9. Page: 1209-1213.
Yunhua W, Xiaobo J, Shengshui H. Studies on electrochemical oxidation of azithromycin and its interaction with bovine serum albumin. Bioelectrochemistry Year: 2004. Volume: 64. Page: 91-97.
Hegde RN, Kumara Swamy BE, Shetti NP, Nandibewoor ST. Electro-oxidation and determination of gabapentin at gold electrode. J. Electroanal. Chem. Year: 2009. Volume: 635. Page: 51-57.
Swatz ME, Krull IS. Analytical Method Development and Validation, Marcel Dekker, New York; 1997.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.