PROTECTIVE EFFECT OF THYMOQUINONE ON THE LIVER TISSUES OF 7, 12-DIMETHYLBENZ (A) ANTHRACENE INDUCED EXPERIMENTAL BREAST CANCER RATS
Abstract
Objective: Thymoquinone (TQ), the main active ingredient of the volatile oil isolated from the Nigella sativa seeds has been shown to possess a wide
array of pharmacological effects. Recently, we have reported the anticancer potential of TQ in 7, 12-dimethylbenz (a) anthracene (DMBA) induced
rats. DMBA acts as a potent site and organ-specific carcinogen by generating various reactive metabolic intermediates leading to oxidative stress. The
present study was hypothesized to explore the protective effect of TQ against DMBA induced liver injury in rats.
Methods: DMBA was used to induce breast cancer in rats. Oral treatment of TQ (25 mg/kg body weight) to DMBA induced rats daily for 4 weeks was
found to be effective against DMBA induced mammary gland carcinogenesis in female Wistar rats. The levels of nucleic acids, oxidative stress mark
and antioxidants were determined. The activities of Phase I and Phase II enzymes, tricarboxylic acid (TCA) cycle enzymes were assayed.
Results: The increased levels of DNA and RNA were found to be decreased on treatment with TQ. The altered activities of Phase I and Phase II
biotransformation enzymes were found to be regulated on treatment with thymoqionone. The hepatoprotective nature of TQ was assessed by
analyzing the markers of oxidative stress, and antioxidant competence in DMBA induced rats. Treatment with TQ revealed a significant decline in the
levels of lipid peroxides, and a significant improvement in the levels of enzymatic and non-enzymatic antioxidants in the liver tissue. In addition, TQ
modulated the activities of TCA cycle enzymes.
Conclusion: The results of the present study clearly indicate that TQ protects the tissues from oxidative stress-mediated damage which is evident
from improved antioxidant status.
Keywords: 7, 12-dimethylbenz (a) anthracene, Thymoquinone, Oxidative stress, Liver tissues, Antioxidant potential.
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