EVALUATION OF PHENOL CONTENT, ANTIOXIDANT, AND PROTEINASE INHIBITORY ACTIVITY OF PLANT DERIVED PROTEASE INHIBITORS OF EIGHT ANTI-DIABETIC PLANTS
Abstract
Objective: The objective of this study was to partially purified from the eight known anti-diabetic plants and evaluated for the total phenol, protease
inhibitory (PI) activity, and antioxidant activity.
Methods: PI activity was performed using casein as substrate. The total phenolic content was estimated by folin-ciocatechu reagent method. The
antioxidant activity was estimated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) model and hydrogen peroxide scavenging activity.
Results: The results shown that Syzygium cumini has the highest total phenol and protein content. Solanum tuberosum has the highest percentage
PI activity. In DPPH model of antioxidant in SC has the highest inhibitory activity, whereas in the hydrogen peroxide scavenging activity Momardica
charantia has the maximum inhibition.
Conclusion: These data once again prove that phenolic compounds have statistically significant antioxidant activity. Among all the PIs ST has
maximum inhibition activity. To conclude, all these PIs can be an excellent source of antioxidants.
Keywords: Antioxidant, Plant protease inhibitor, Phenolic, Hydrogen peroxide, Protein content.
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References
Fang SG, Guo JW. Characteristics of plant proteinase inhibitors and
their applications in combating phytophagous insects. Bot Bull Acad
Sin 2005;46:273-92.
Antunes E, Oliveira AD, Maria L, Rabelo A, Uchoa AF, Heloneida A,
et al. Affinity chromatography as a key tool to purify protein
protease inhibitors from plants. In: Magdeldin S, editor. Affinity
Chromatography. Croatia: InTech; 2012. p. 211-44.
Telang M, Srinivasan A, Patankar A, Harsulkar A, Joshi V, Damle A,
et al. Bitter gourd proteinase inhibitors: Potential growth inhibitors
of Helicoverpa armigera and Spodoptera litura. Phytochemistry
;63:643-52.
Birk Y. Protease inhibitors of plant origin and role of protease inhibitors
in human nutrition: An overview. In: Troll W, Kennedy AR, editors.
Protease Inhibitors as Cancer Chemopreventive Agents. New York:
Plenum Press; 1993. p. 97-106.
Abdel-Medguid M, Von der Helm K, Korant BD, Cheronis JC.
Proteases as Targets for Therapy. Berlin: Springer-Verlag; 2000.
Leung D, Abbenante G, Fairlie DP. Protease inhibitors: Current status
and future prospects. J Med Chem 2000;43(3):305-41.
Hatano T, Edamatsu R, Mori AF, Yasuhara E. Effect of interaction
of tannins with co-existing substances. Effect of tannins and related
polyphenols on superoxide anion radical and on DPPH radical. Chem
Pharm Bull 1989;37:2016-21.
Duh PD, Tu YY, Yen GC. Antioxidant activity of water extract of
harngjyur (Chrysanthemum morifolium Ramat). Lebnesmittel-Wiss
Technol 1999;32:269-77.
Tanaka M, Kuei GW, Nagashima Y, Taguchi T. Application of
antioxidative maillard reaction products from histidine and glucose to
sardine products. Nippon Suisan Gakkashi 1998;54:1409-14.
Bijina B, Chellappan S, Krishna JG, Basheer SM, Elyas KK,
Bahkali AH, et al. Protease inhibitor from Moringa oleifera with
potential for use as therapeutic drug and as seafood preservative. Saudi
J Biol Sci 2011;18(3):273-81.
Capannesi C, Palchetti I, Mascini M, Parenti A. Electrochemical sensor
and biosensor for polyphenols detection in olive oils. Food Chem
;71(4):535-62.
Lu Y, Foo LY. Antioxidant and radical scavenging activities of
polyphenols apple pomace. Food Chem 2000;68:81-5.
Burda S, Oleszek W. Antioxidant and antiradical activities of flavonoids
J Agric Food Chem 2001;49(6):2774-9.
Amarowicz R, Pegg RB, Rahimi-Moghaddam P, Barl B, Weil JA. Free
radical scavenging capacity and antioxidant activity of selected plant
species from the Canadian prairies. Food Chem 2004;84:551-62.
Keser S, Celik S, Turkoglu S, Yilmaz O, Turkoglu I. Hydrogen peroxide
radical scavenging and total anti-oxidant activity of Hawthorn. Chem J
;2(1):9-12.
Smith MA, Rottkamp CA, Nunomura A, Raina AK, Perry G.
Oxidative stress in Alzheimer’s disease. Biochim Biophys Acta
;1502(1):139-44.
Kinnula VL, Crapo JD. Superoxide dismutases in malignant cells and
human tumors. Free Radic Biol Med 2004;36(6):718-44.
Singh U, Jialal I. Oxidative stress and atherosclerosis. Pathophysiology
;13(3):129-42.
Sas K, Robotka H, Toldi J, Vécsei L. Mitochondria, metabolic
disturbances, oxidative stress and the kynurenine system, with focus on
neurodegenerative disorders. J Neurol Sci 2007;257(1-2):221-39.
Bolton JL, Trush MA, Penning TM, Dryhurst G, Monks TJ. Role of
quinones in toxicology. Chem Res Toxicol 2000;13(3):135-60.
Ramakrishna BS, Varghese R, Jayakumar S, Mathan M,
Balasubramanian KA. Circulating antioxidants in ulcerative colitis
and their relationship to disease severity and activity. J.Gastroenterol.
Hepatol 1997;12(7):490-4.
Yen GC, Duh PD. Scavenging effect of methanolic extracts of
peanut hulls on free radical and active oxygen. J Agric Food Chem
;42(3):6-9.
Asian J Pharm Clin Res, Vol 9, Issue 3, 2016, 215-219
Mayasa et al.
Zheng W, Wang SY. Antioxidant activity and phenolic compounds in
selected herbs. J Agric Food Chem 2001;49(11):5165-70.
Fear G, Komarnytsky S, Raskin I. Protease inhibitors and their
peptidomimetic derivatives as potential drugs. Pharmacol Ther
;113(2):354-68.
Duh PD. Antioxidant activity of burdock (Arctiumlappa Linne): Its
scavenging effect on free radical and active oxygen. J Am Oil Chem
Soc 1998;75;455-65.
Hung CH, Huang CC, Tsai WS, Wang HL, Chen YL. Purification and
characterization of a trypsin inhibitor from Brassica campestris seeds.
J Yuanpei Univ Sci Technol 2003;10:13-22.
Mosolov VV, Grigor Eva LI, Valueva TA. Plant proteinase inhibitors as
multifunctional proteins. Appl Biochem Microbiol 2001;37(6):643-50.
Shewry PR. Tuber storage proteins. Ann Bot 2003;91(7):755-69.
De Leo F, Gallerani R. The mustard trypsin inhibitor 2 affects the
fertility of Spodoptera littoralis larvae fed on transgenic plants. Insect
Biochem Mol Biol 2002;32(5):489-96.
Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human
health and disease. Oxid Med and Cellular Longe 2009;2(5):270-278.
Halliwell B. Free radicals and metal ions in health and disease. Proc
Nutr Soc 1987;46(1):13-26.
Lewis NG. Plant phenolics. In: Alscher RG, Hess JL, editors.
Antioxidants in Higher Plants. Boca Raton, FL: CRC Press; 1993. p.
-69.
Banerjee A, Dasgupta N, De B. In vitro study of antioxidant activity of
Syzygium cumini fruit. Food Chem 2005;90(4):727-33.
Patel S, Patel T, Parmar K, Patel B, Patel P. Evaluation of antioxidant
activity, phenol and flavonoid contents of Momordica charantia Linn
fruit. ARPB 2011;1(2):120-9.
Raza MA, Shahwar D, Khan T. Radical scavenging, proteases activities,
and phenolics composition of bark extracts from 21 medicinal plants. J
Chem 2015:1-8.
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