PHYTOCHEMICAL, ANTIOXIDANT, AND FREE RADICAL SCAVENGING ACTIVITIES OF HYDROETHANOLIC EXTRACT OF AERIAL PARTS OF POTHOS SCANDENS L.
Abstract
Objective: The present study was carried out to evaluate the phytochemical, antioxidant, and free radical scavenging activity of 50% hydro-ethanolic
extract of Pothos scandens L.
Methods: To evaluate the total phenolics, alkaloid, and flavonoid content of 50% hydro-ethanolic extract of P. scandens and also to assess the antioxidant
and free radical (2, 2 diphenyl-1-picryl hydrazine radicals, hydroxyl radical, superoxide radical, and nitric oxide radical) scavenging activity, inhibition
of lipid peroxidation (LPO), and total antioxidant activity of the plant extract. All experiments were performed in triplicate (n=3), and the results were
expressed as mean±stanadard deviation.
Results: The result indicated that the plant contained more amounts of total phenolic content, followed by alkaloid and flavonoid content. The plant
extracts showed effective radical scavenging activity and showed pronounced inhibitory action against LPO.
Conclusion: The study concluded that 50% ethanolic extract of P. scandens L. has showed the significant antioxidant property. This extract can
therefore be used to screen its potential activities against many diseases which are caused by free radicals.
Keywords: Pothos scandens L., 2, 2 diphenyl-1-picryl hydrazine, Total phenolics.
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