EFFECT OF ETHANOLIC EXTRACT OF CALOPHYLLUM INOPHYLLUM LEAVES ON OXIDATIVESTRESS COMPLICATIONS IN MOUSE MODEL
Abstract
Objectives: To find out the effect of the extract of Calophyllum inophyllum leaves on the streptozotocin-induced oxidative stress complications in mice.
Methods: The plant extract was first checked for its radical scavenging activity by the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) method and
L-ascorbic acid was used as the standard. DPPH assay activity of the plant extract was found to be close to the standard drug ascorbic acid. Acute
toxicity was conducted as per OECD 425 guidelines. From the results obtained, 250, 300, and 350 mg/kg dose were chosen for further experimentation.
After 16 days of drug treatment, glucose, cholesterol, triglyceride and enzymatic antioxidants levels were estimated in serum samples.
Results: C. inophyllum leaves extract has significantly reduced the glucose, cholesterol, triglyceride and enzymatic antioxidants levels. Hence, this
proves that the plant has anti-diabetic property. However, vitamin E had no effect on the triglyceride level. Antioxidant activity was monitored by
superoxide dismutase, catalase, glutathione peroxidase, malondialdehyde assay and it was found that the plant extract has effectively increased the
antioxidant activity as the dose increases.
Conclusion: C. inophyllum leaves extract have anti-diabetic activity and effective in curbing the oxidative stress complications.
Keywords: Calophyllum inophyllum, Oxidative stress complications, Anti-diabetic activity, Free radicals.
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