ANALYSIS OF SESAME PROTEINS ISOLATE (SESAMUM INDICUM L) WITH WATER AND SALT TREATMENT
Abstract
Objective: The aim of this study was to obtain protein isolate from sesame using alkaline pH at different pHs of precipitation with water and salt and
to analyze protein isolate with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Methods: Sesame protein isolates were obtained using isoelectric precipitation method at different pHs using water and salt as solvents. Proteins
were analyzed using native-PAGE and SDS-PAGE.
Results: A yield of 14,727% ± 0.3 of protein isolate of defatted sesame flour at pH 7.0 with a 47.4% ± 0.6 of protein was obtained. The yield of protein
isolate using water and salt was similar. Polypeptides profile is between 6.5 and 50 kDa.
Conclusions: Sesame seed is a good source of proteins. Globulins and albumins were identified in the sesame protein isolate in the presence of water
and salt.
Keywords: Sesame, Protein isolate, Proteins, Globulins and albumins.
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