STUDIES ON ANTIOXIDANT ACTIVITY, PHENOL AND FLAVONOID CONTENT OF PISONIA ALBA SPAN.

Abstract

Pisonia alba leaf were collected from Five different biodiversity of Tamilnadu to Analysis the Antioxidant activity, Total Phenol and Flavonoid content , leaf extraction from various solvents( Aqueous, Ethanol, Petroleum ether, Chloroform, and acetone). Butylated Hydroxy Toluene (BHT), Gallic acid (GA) and Quercetin (Q) were taken as standard in case of antioxidant activity, phenol and flavonoid content respectively. The leaf extracts were evaluated for antioxidant activities by DPPH (1,1– diphenyl -2- picryl - hydrazyl) radical scavenging assay. The Maximum antioxidant activity was found in ethanolic leaf extract (65.7%) from Thiruvannamalai as compare to other accessions. Total phenol and flavonoid contents were quantitatively estimated. Total phenolic content measured by Folin-Ciocalteau method varied from 9.64 to 29.72 mg .Gallic Acid Equivalents (GAE)/g and the total flavonoid contents as measured by aluminum chloride method varied from 6.32 to 12.36 mg Quercetin Equivalents (QE)/g.The ethanolic leaf extract of pisonia alba was found maximum in total phenol and flavonoid contents were 12.6 mg GAE /g and 7.5 mg QE /g respectively.

Key words: Pisonia alba, antioxidant activity, DPPH, phenol and flavonoid.