IN VITRO ANTIBIOFILM ACTIVITY OF GYMNEMA SYLVESTRE EXTRACT AGAINST BIOFILM FORMING STREPTOCOCCUS PYOGENES FROM UPPER RESPIRATORY TRACT

Abstract

ABSTRACT
Objective: This study was aimed to evaluate the antibiofilm activity of Gymnema sylvestre solvent extracts against the biofilm forming Streptococcus
pyogenes strains SP-1 and SP-2 isolated from pharyngitis patients.
Methods: The study involved the determination of minimum inhibitory concentration (MIC) and biofilm inhibitory concentration using microtiter
plate method, growth curve analysis using spectrophotometry, antibacterial activity using agar well-diffusion method and light microscopic analysis
of S. pyogenes biofilms treated with the plant extracts.
Results: Biofilm inhibition assay was showed that the G. sylvestre methanol and ethyl acetate extracts inhibited biofilms at sub-MIC which was also
confirmed via light microscopy. Minimal inhibition concentration against the tested pathogens was observed at 4 mg/ml for both methanol and ethyl
acetate extracts. The antibacterial and growth curve analysis showed no significant inhibition at the concentration below MIC.
Conclusion: This study is an attempt to highlight G. sylvestre as a novel antibiofilm source, as the plant is well known for its biologically active
components. Identification and can develop a scientific base for the use in modern medicine.
Keywords: Antibiofilm, Biofilm inhibitory concentration, Gymnema sylvestre, Streptococcus pyogenes.