SUBCHRONIC TOXICITY TEST OF COMBINATION ETHANOL EXTRACT OF DETAM 1 SOYBEAN (GLYCINE MAX L. MERR) AND JATI BELANDA (GUAZUMA ULMIFOLIA LAMK) TOWARD  FUNCTION, WEIGHT, AND HISTOPATHOLOGICAL OF WISTAR RAT LIVER

Meilinah Hidayat; Sijani Prahastuti; Estherolita Dewi; Dewi Safitri; Siti Farah Rahmawati; Andreanus A Soemardji

doi:10.22159/ajpcr.2016.v9i5.13237

Authors

Meilinah Hidayat Maranatha Christian University
Sijani Prahastuti Maranatha Christian University
Estherolita Dewi Maranatha Christian University
Dewi Safitri Institut Teknologi Bandung
Siti Farah Rahmawati Institut Teknologi Bandung
Andreanus A Soemardji Institut Teknologi Bandung

DOI:

https://doi.org/10.22159/ajpcr.2016.v9i5.13237

Abstract

Objective: As an antiobesity therapy, combination extracts of Detam 1 soybean and Jati Belanda will be consumed for a long time; therefore, their
toxicities to the liver need to be investigated. To determine the effect of subchronic toxicity test of combination of ethanol extract of Detam 1 soybean
(EEDS) and ethanol extract of Jati Belanda (EEJB) on liver function with parameters: Alanine transaminase (ALT), macroscopic, and histopathological
of liver.

Methods: This study was conducted on 120 Wistar rats (60 males and 60 females), 90 days (treatment group) and 120 days (satellite group). Rats
were divided into six treatment groups (3 test materials, 1 control, and 2 satellites); each group included 10 males and 10 females.

Results: ALT levels of treatment groups (low dose, medium, and high), both males and females were lower than the control group (p<0.05). The
treatment groups demonstrated a good effects effect on liver function. Liver weight of all groups showed no significant difference compared with the
control group (p>0.05). Results of histopathological score interpretation of male and female liver rats of low dose groups were not disturbed; middle
dose groups were slightly disturbed and high dose groups were damaged. Satellite high doses of male groups were disrupted, while female groups
were not.

Conclusion: The combination of EEDS and EEJB has a good effect on liver function, did not lead to change organ weight and at low doses did not cause
renal histopathology damage in rats after 90 days administration.

Keywords: Combination of soybean Jati Belanda, Toxicity subchronic test, Function, Weight, Histopathology, Liver.

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Author Biographies

Meilinah Hidayat, Maranatha Christian University

Nutrition Department

Sijani Prahastuti, Maranatha Christian University

Biochemistry Department

Dewi Safitri, Institut Teknologi Bandung

Pharmacology and Clinical Pharmacy Research Group

Siti Farah Rahmawati, Institut Teknologi Bandung

Pharmacology and Clinical Pharmacy Research Group

Andreanus A Soemardji, Institut Teknologi Bandung

Pharmacology and Clinical Pharmacy Research Group

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