CHEMOTHERAPEUTIC EFFICACY OF TRIDHAM AND 1,2,3,4,6-PENTA-O-GALLOYL-β-DGLUCOSE ON ANTIOXIDANTS STATUS AND TUMOR MARKERS IN EXPERIMENTAL MAMMARY CARCINOMA IN SPRAGUE-DAWLEY RATS
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i5.13247Abstract
Objective: To study the restorative effect of Tridham (TD) and 1,2,3,4,6-penta-o-galloyl-β-D-glucose (PGG) on 7,12-dimethyl benz(a)anthracene
(DMBA)-induced mammary carcinoma in female Sprague-Dawley rats.
Methods: Rats were divided into seven groups of six animals each. Group I rats served as control. Group II - mammary carcinoma was induced
by DMBA. Group III and Group IV were induced with DMBA and subsequently treated with TD and PGG, respectively, for 48 days. Group V was
treated with DMBA and subsequently with a standard drug, cyclophosphamide (CYC). Group VI and Group VII were given TD and PGG alone,
respectively, for 48 days. After the experimental period, the levels of lipid peroxides (LPO), activities of enzymic and non-enzymic antioxidants
such as superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, reduced glutathione, vitamin C, and vitamin E were
evaluated in the breast tissue of control and experimental rats. Levels of LPO, marker enzymes such as 5'-nucleotidase and lactate dehydrogenase,
were also evaluated.
Results: The levels of enzymic and non-enzymic antioxidants were decreased in DMBA-induced rats when compared to control rats. The levels of
tumor markers were increased in DMBA-induced rats when compared to control rats. These parameters were restored to near normal levels on
treatment with TD and PGG.
Conclusions: The results suggest that TD and PGG have a cytoprotective role in DMBA-induced breast cancer-bearing rats. The effect of TD and PGG
was found to be more pronounced than CYC, a standard drug.
Keywords: Breast cancer, Tridham, Penta galloyl glucose, Antioxidants, Tumor markers, Sprague-Dawley rats.
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