ANTIOSTEOPOROTIC ACTIVITY OF ANTHRAQUINONE ISOLATED FROM MORINDA CITRIFOLIA FRUITS IN RATS
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i5.13269Abstract
Objectives: This study was designed to investigate the antiosteoporotic activity of isolated anthraquinones from Morinda citrifolia fruit extract in
ovariectomy (OVX) induced osteoporotic rats.
Methods: All the rats were divided into 4 groups (n=6 each). Group I (sham control) received vehicle, p.o., Group II OVX control (vehicle, p.o.),
Group III was OVX+standard raloxifene (5.4 mg/kg, p.o.), and Group IV was OVX+Physcion (100 mg/kg, p.o.) for 90 days.
Results: The daily oral administration of isolated compound physcion (100 mg/kg) for 12 weeks to the rats prevented OVX-induced osteoporosis.
This was examined by serum biomarkers such as alkaline phosphatase, calcium, and tartrate resistant acid phosphatase and showed significant
effects (p<0.0001). The femur bone strength assessed by three-point bending test showed improved bone strength in physcion treated rats, and
this was supported by enhanced bone mineral density (p<0.05). The ash parameters of femur bone studied from physcion treated rats exhibited a
significant (p<0.0001) value of ash weight followed by ash calcium content. Further, femur bone histological examination revealed the protective
effect of the compound physcion (100 mg/kg) against OVX-induced bone loss in rats, where it showed mineralization of trabecular spaces, improved
bone compactness thereby intact bone architecture.
Conclusion: This study concludes that the isolated anthraquinone physcion had a preventive effect against OVX-induced bone loss in rats.
Keywords: Morinda citrifolia, Physcion, Osteoporosis, Bone mineral density, Ash mineral content.
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