EXTRACELLULAR AND INTRACELLULAR SYNTHESIS OF SILVER NANOPARTICLES
DOI:
https://doi.org/10.22159/ajpcr.2016.v9s2.13302Abstract
ABSTRACT
Objective: The cellular synthesis of nanoparticle is a green process and alternative for a conventional process for the preparation of silver nanoparticles.
In our research, focus has been given to the development of an efficient and eco-friendly viable process for the synthesis of silver nanoparticles using
cancer and non-cancerous cells, a cell culture that was isolated. The results of this investigation are observed that silver nanoparticles could be
induced to synthesis intra- and extra-cellularly using mammalian cells such as cancerous and non-cancerous cells.
Methods: The silver nanoparticles are synthesized by the cancer and non-cancerous cells such as HeLa (Homo sapiens, human), SiHa, and human
embryonic kidney-293 cell lines. The silver nanoparticles were characterized by ultraviolet (UV)-visible spectroscopy, transmission electron
microscopy (TEM), and X-ray powder diffraction (XRD).
Results: The silver nanoparticles exhibited maximum absorbance at 415 nm in UV-visible spectroscopy. The XRD confirms the characteristic of the
crystal lattice of silver nanoparticles by observing three peaks: Peak at 38 is due to reflection from (111), peak at 44 is due to reflection from (200),
and peak at 65 is due to reflection from (220). TEM images showed the formation of stable silver nanoparticles in the cell lines.
Conclusion: The method of extraction of intracellular/extracellular synthesis of silver nanoparticles was inexpensive, simple, and effective in large
scale with no need to use of complex process equipment. The cancer cell considered as a biological factory at nanoscale dimension which continued to
grow after synthesis of silver nanoparticles. The silver reduction by these cancer cells has occurred through energy-dependent processes that lead to
the high output of this reaction. Hence, this new approach of using a mammalian cell for the successful synthesis of nanosized silvers could be easily
scaled up, which establishes its commercial viability and also useful in the drug delivery and drug targeting.
Keywords: Silver nanoparticles, Cancer cells, Biosynthesis and characteristics of silver nanoparticles.
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