ISOLATION OF TAXOL PRODUCING FUSICOCCUM SPECIES FROM CURRY LEAF AND ITS RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS
DOI:
https://doi.org/10.22159/ajpcr.2016.v9s2.13544Abstract
ABSTRACT
Objective: The potential compound of taxol has been used treatment of cancer in the medical field. This study is focused on the extraction of taxol
from Fusicoccum sp.
Methods: The pathogenic strain was grown in MID medium for the taxol production and extracted from the Fusicoccum culture filtrate and processed
to the ultraviolet (UV)-spectroscopy analysis as well as infrared (IR) spectra analysis was done, and the sample was performed to the high-performance
column chromatography and further processed to the random amplified polymorphic DNA (RAPD) analysis of specific primer is PGFO1, PGFO2 are
performed.
Results: UV-visible spectral analysis showed maximum value for 273 nm. The result of IR-spectrum analysis to find out the functional groups present
in sample and characteristic peak to conform the existences of OH group. The presence of taxol was confirmed using column chromatography.
An analysis of taxol production was on the external standard of authentic taxol. The column chromatography process shows the amount of taxol
production by Fusicoccum spp. 325 µg/L. RAPD analysis indicates the genetic relation among all isolates of fungus was analyzed by two random
primers PGF01 and PGF02.
Conclusion: The present investigation revealed that the taxol production from biological source was a significant effect, and the presence of taxol
amount is analysis in Fusicoccum sp. 325 µg/L. The RAPD analysis of genetic stability of in vitro grown fungus and genotypic with two different
primers shows that five fungus species with corresponding primer efficiency was amplified.
Keywords: Taxol production, Fusicoccum sp., Ultraviolet-spectroscopy, Infrared, Spectrum analysis, Scanning electron microscopy.
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REFERENCES
Chakravarthi BV, Das P, Surendranath K, Karande AA, Jayabaskaran C.
Production of paclitaxel by Fusarium solani isolated from Taxus
celebica. J Biosci 2008;33(2):259-67.
Visalakchi S, Muthumary J. Taxol (anticancer drug) producing
endophytic fungi an overview. Int J Pharm Bio Sci 2010;1(3):1-9.
Kathiravan G. Muthumary J. Extraction of anticancer drug from
coelomycetous fungi Pestalotiopsis versicolor and Phyllosticta
murrayicola. Mycol Balcanica 2009;6:55-60.
Witcher W, Clayton CN. Bluberry stems blight caused by Batryosphaeria
dothiden B. Ribis. Phytopathology 1963;53:705-12.
English H, Davis JR, Devay JE. Relationship of Botryosphaeria
dothidea and Hendersonula toruloidea to a canker disease of almond.
Phytopathology 1975;65(2):114-22.
Brown EA, Hendrix FF. Pathogenicity and histopathology
of Batryosphaeria dothidea on apple stems. Phytopathology
;71(4):375-9.
Mass JL, Uecker FA. Botryosphaeria dothidea cane canker of thornless
blackberry. Plant Dis 1984;68:720-6.
Rumbos C. Twing and Branch Dieback of Walnut Tree Induled by
Statistics Ver. 5
ed. Cary, NC: SAS Inc.; 1987. p. 584.
Stierle A, Strobel G, Stierle D. Taxol and taxane production by
th
Taxomyces andreanae, an endophytic fungus of Pacific yew. Science
;260(5105):214-6.
Hoffmeister D, Keller NP. Natural products of filamentou0073
fungi: Eenzymes, genes, and their regulation. Nat Prod Rep
;24(2):393-416.
Strobel GA, Hess WM, Ford E, Sidhu RS, Yang XA. Taxol from fungal
endophytes and the issue of biodiversity. J Indian Microbiol Biotechnol
;17(5):417-23.
Kusari S, Lamshöft M, Spiteller M. Aspergillus fumigatus Fresenius,
an endophytic fungus from Juniperus communis L. Horstmann as a
novel source of the anticancer pro-drug deoxypodophyllotoxin. J Appl
Microbiol 2009;107(3):1019-30.
Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT. Plant
antitumor agents. VI. The isolation and structure of taxol, a novel
antileukemic and antitumor agent from Taxus brevifolia. J Am Chem
Soc 1971;93(9):2325-7.
Shi YL. Loomis P, Christian D, Carris LM, Leung H. Analysis of the
genetic relationship among the wheat bunt fungi using RAPD and
ribosomal DNA markers. Phytopathology 1996;86(3):311-8.
Li C, Yeh FC, Hiratsuka Y. Random amplified polymorphic DNA
variability among geographic isolates of Western gall rust fungus in
Canada. J For Res 2001;31(8):1304-11.
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA
polymorphisms amplified by arbitrary primers are useful as genetic
markers. Nucleic Acids Res 1990;18(22):6531-5.
Demeke T, Adams RP. The use of PCR-RAPD analysis plant
taxonomy and evaluation. In: Griffin HG, Griffin AM, editors. PCR
Technology: Current Innovation. Boca Raton, FL: CRC Press; 1994.
Senyuva HZ. Rapid Analysis of Crude Fungal Extraction for
Secondary Metabolite by LC/TOF-MS-A New Approach of Fungal
Characterization. Scientific and Technological Research Council of
Turkey; 2007.
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