FOURIER TRANSFORM INFRARED SPECTROSCOPY SPECTROSCOPIC STUDIES IN EMBELIA RIBES BURM. F.: A VULNERABLE MEDICINAL PLANT
DOI:
https://doi.org/10.22159/ajpcr.2016.v9s3.13804Abstract
ABSTRACT
Objective: The present study was aimed to identify the functional group present in the crude powder and various solvent extracts of Embelia ribes
Burm. f. stem, leaves, and berries through Fourier transform infrared (FTIR) spectroscopy.
Methods: Different plant parts of E. ribes were collected shade dried, powdered, and extracted in methanol, ethanol, and petroleum ether. These
extracts were used to detect the characteristic peak values and their functional groups using FTIR method on a OMNI sampler attenuated total
reflectance accessory on a JASCO FTIR spectrophotometer (FTIRâ€4600).
Results: The crude powder of E. ribes leaves, stem, and berries FTIR analysis confirmed the presence of amino acids, amide, alkanes, carboxylic
acids, alcohols, esters, ethers, aromatics, aliphatic amines, phenols, aldehyde, ketones, fluorides, halogen, alkyl halides, and nitro compound. The dry
methanolic and ethanolic extracts of E. ribes leaves, stem, and berries FTIR analysis results proved the presence of alcohols, p-substituted alcohols
or phenols, phenols, alkanes, alkynes, alkenes, aldehyde, ester, ether, aliphatic amines, carboxylic acids, aromatics, ketones, disulphide, alkyl halides,
halogen, and nitro compounds, whereas dry petroleum ether extract shown the presence of amide, alkanes, carboxylic acids, alcohols, p-substituted
alcohols or phenols, esters, aromatics, aldehyde, ketones, aryl disulphide, aliphatic amines, aliphatic compound, alkyl halides, and nitro compounds,
respectively.
Conclusion: The results of the present study produced the FTIR spectrum profile for the vulnerable medicinally important plant E. ribes Burm. f.
Keywords: Embelia ribes Burm. f., Fourier transform infrared spectroscopy, Spectroscopy, Functional groups.
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References
Surewicz WK, Mantsch HH, Chapman D. Determination of protein secondary structure by Fourier transform infrared spectroscopy: A critical assessment. Biochemistry 1993;32(2):389-3.
McCann MC, Hammouri M, Wilson R, Belton P, Roberts K. Fourier transform infrared microspectroscopy is a new way to look at plant cell walls. Plant Physiol 1992;100:1940-7.
Yang J, Yen HE. Early salt stress effects on the changes in chemical composition in leaves of ice plant and Arabidopsis. A Fourier transform infrared spectroscopy study. Plant Physiol 2002;130(2):1032-42.
Ivanova DG, Singh BR. Nondestructive FTIR monitoring of leaf senescence and elicitin induced changes in plant leaves. Biopolymers 2003;72(2):79-85. Available from: http://www.onlinelibrary.com/doi/10.1002/bip.10297/pdf.
Marimuthu M, Gurumoorthi P. Phytochemical screening and FTIR studies on wild and common south Indian legumes. Asian J Pharm Clin Res 2013;6(2):141-4.
Ashokkumar R, Ramaswamy M. Phytochemical screening by FTIR spectroscopic analysis of leaf extracts of selected Indian medicinal plants. Int J Curr Microbiol Appl Sci 2014;3(1):395-6.
Stehfest K, Toepel J, Wilhelm C. The application of micro-FTIR spectroscopy to analyze nutrient stress-related changes in biomass composition of phytoplankton algae. Plant Physiol Biochem 2005;43(7):717-26.
Muruganatham S, Anbalagan G, Ramamurthy N. FTIR and SEM-eds comparative analysis of medicinal plants. Eclipta alba HASSK and Eclipta prostrata Linn. Rom J Biophys 2009;19(4):285-94.
Ravikumar K, Ved DK. 100 Red Listed Medicinal Plants of Conservation Concern in South India. Bangalore, India: FRLHT; 2000. Available from: https://www.books.google.co.in/books/about/100_red_listed_medicinal_plants_of_conse.html?id=EUQPAQAAMAAJ.
Mhaskar M, Joshi S, Chavan B, Joglekar A, Barve N, Patwardhan A. Status of Embelia ribes Burm. f. an important medicinal species of commerce from northern Western Ghats of India. Curr Sci 2011;100(4):547-52.
Sivarajan VV, Balachandran I. Ayurvedic Drugs and Their Plant Sources. Oxford and IBH Pub., Co., Pvt., Ltd., India. 1994. p. 267-96. Available from: https://www.books.google.co.in/books/about/Ayurvedic_Durgs_and_their_plant_source.html?id=nQCmj2PO9gAC.
Gupta OP, Ali MM, Ray Ghatak BJ, Atal CK. Some pharmacological investigations of embelin and its semisynthetic derivatives. Indian J Physiol Pharmacol 1977;21(1):31-9.
Du YC, Wie JS. Study of vermifuges. I. Isolation of embelin from the fruit of Mugua-wha Embelia oblongifolia Hemsl.). Yao Xue Xue Bao 1963;10:578-80.
Dallacker F, Löhnert G. Derivatives of methylenedioxybenzene 35. A novel synthesis of 3,6 dihydroxy-2-ethyl-1,4-benzoquinone, embelin, vilangin, rapanone, dihydromaesaquinone, bhogatin, spinulosin and oosporein. Chem Ber 1972;105(2):614-24.
Chitra M, Sukumar E, Suja V, Devi CS. Antitumor, anti-inflammatory and analgesic property of embelin, a plant product. Chemotherapy 1994;40(2):109-13.
Kim SW, Ban SH, Chung H, Cho S, Chung HJ, Choi PS, et al. Taxanomic discrimination of flowering plants by multivariate analysis of Fourier transform infrared spectroscopy data. Plant Cell Rep 2004;23:246-50.
Komal Kumar J, Devi Prasad AG. Identification and comparison ofbiomolecules in medicinal plants of Tephrosia tinctoria and Atylosia albicans by using FTIR. Rom J Biophys 2011;21(1):63-71.
Liu H, Sun S, Lv G, Chan KK. Study on Angelica and its different extracts by Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy. Spectrochim Acta Part A 2006;64:321-6.
Yamunadevi M, Wesely EG, Johnson M. FTIR spectroscopic studies on Aerva lanata (L.) Juss. Ex Schult. Asian J Pharm Clin Res 2012;5(2):82-6.
Bobby MA, Wesely EG, Johnson M. FT-IR studies on the leaves of Albizia lebbeck Benth. Int J Pharm Pharm Sci 2012;4(3):293-6.
Thangarajan S, Paramasivam R, Chinthamony AR, Palanisamy CP, Velliyur KG. Element and functional group analysis of Ichnocarpus frutescens R. Br. (Apocynaceae). Int J Pharm Pharm Sci 2012;4(5):343-5.
Srinivasan P, Dineshbabu J, Manimekalai K, Priya DD. Spectroscopic analysis and antibacterial efficacy of bioactive compounds from Limonia acidissima L. fruit extract against clinical pathogens. Int J Pharm Pharm Sci 2015;7(3):383-9.
Saravana PP, Gopalakrishnan VK. Phytochemical screening, functional groups and elemental analysis of leaf extract of Ipomoea obscura (L) ker-gawl. Int J Pharm Pharm Sci 2014;6(9):83-9.
Sundaram S, Palanisamy CP, Velliyur KG. Chromatographic and spectrophotometric analysis of bioactive compounds from Cayratia trifolia (L) stem. Int J Pharm Pharm Sci 2016;8(6):56-64.
Jayashree VH, Ramesh L. Isolation and identification of a flavone from fruit pulp of Feronia limonia. Int J Curr Pharm Res 2014;6(4):28-31.
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