ANTIOXIDANT AND FREE RADICAL SCAVENGING CAPACITY OF EXTENSIVELY USED MEDICINAL PLANT OF PUNICA GRANATUM

VINODHINI S; SHRI PREETHI M; NUSRATH FATHIMA N; SHIVANI S KUSHWAHA; Devi Rajeswari v

doi:10.22159/ajpcr.2016.v9i6.13941

Authors

VINODHINI S
SHRI PREETHI M
NUSRATH FATHIMA N
SHIVANI S KUSHWAHA
Devi Rajeswari v VIT University

DOI:

https://doi.org/10.22159/ajpcr.2016.v9i6.13941

Abstract

ABSTRACT

Objective: The present study was aimed to examine the in vitro antioxidant property and free radical scavenging capacity of Punica granatum leaves, which are widely used in the Ayurvedic treatment.

Methods: Aqueous and methanol extracts were prepared and to assess for their Phytochemical analysis to find out the various compounds and total yield of phenolic and flavonoid contents by spectrophotometer methods. Free-radical scavenging capacity and antioxidant activity was estimated by using a number of chemical assays are DPPH, H₂O₂, FRAP, TRAP and beta carotene assay.

Results: It showed that aqueous extract had greatest antioxidant activity and contained significant levels of total phenolic and flavonoids content expressed as mg of gallic acid and quercetin equivalent/g of extracts. The extracts showed antioxidant activity in vivo by protecting yeast cells against oxidative stressing agent H₂O_2.

Conclusion: These results specified the Punica granatum is good source of natural compounds which might have benefits for health, which can be used in dietary application with a possible to reduce oxidative stress.

Key words:Â Punica granatum, Free-radical scavenging capacity, phytochemical, antioxidant, yeast culture.

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Author Biography

Devi Rajeswari v, VIT University

Dr. V. Devi Rajeswari,

Assistant professor (Senior)

Department of Biomedical sciences

SBST

VIT University

Vellore

References

Halliwell B. Free radicals, antioxidants, and human disease: Curiosity, cause, or consequence? Lancet 1994;344(8924):721-4.

Ani V, Varadaraj MC, Naidu KA. Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum L.). Eur Food Res Technol 2006;224:109-15.

McCord JM. The evolution of free radicals and oxidative stress. Am J Med 2000;108(8):652-9.

Ebrahimzadeh MA, Pourmorad F, Hafezi S. Antioxidant activities of Iranian corn silk. Turk J Biol 2008;32:43-9.

Farber JL. Mechanisms of cell injury by activated oxygen species. Environ Health Perspect 1994;102 Suppl 10:17-24.

Scherer R, Godoy HT. Antioxidant activity index (AAI) by the 2, 2-diphenyl-1-picrylhydrazyl method. Food Chem 2009;112(3):654-8.

Namiki M. Antioxidants/antimutagens in food. Crit Rev Food Sci Nutr 1990;29(4):273-300.

Robak J, Marcinkiewicz E. Scavenging of reactive oxygen species as the mechanism of drug action. Pol J Pharmacol 1995;47(2):89-98.

Wintola OA, Afolayan AJ. Phytochemical constituents and antioxidant activities of the whole leaf extract of Aloe ferox Mill. Pharmacogn Mag 2011;7(28):325-33.

Sen S, Chakraborty R, Sridhar C, Reddy YS, De B. Free radicals, antioxidants, diseases and phytomedicines: Current status and future prospect. Int J Pharm Sci Rev Res 2010;3(1):91-100.

Bourgaud F, Gravot A, Milesi S, Gontier E. Production of plant secondary metabolites: A historical perspective. Plant Sci 2001;161(5):839-51.

Joy PP, Thomas J, Mathew S, Skaria BP. Medicinal Plants. Kerala, India: Kerala Agricultural University; 1998.

Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, et al. Effect of extraction solvent on total phenol content, total flavonoid content and antioxidant activity of Limnophila aromatic. J Food Drug Anal 2014;22(3):296-302.

Sen S, Chakraborty R. The role of antioxidants in human health. In: Silvana A, Hepel M, editors. Oxidative Stress: Diagnostics, Prevention, and Therapy, ACS Symposium Series [M]. Washington, DC: Journal of the American Chemical Society; 2011. p. 1-37.

Seday U, Uzun A, Yilmaz C, Eti S. Production and quality of pollen in terms of fruit set on some self-pollinated pomegranate cultivars, Bulgarian. J Agric Sci 2013;19(3):513-7.

Lei F, Zhang XN, Wang W, Xing DM, Xie WD, Su H, et al. Evidence of anti-obesity effects of the pomegranate leaf extract in high-fat diet induced obese mice. Int J Obes (Lond) 2007;31(6):1023-9.

Harborne JB. Phytochemical Methods. London: Chapman and Hill; 1973.

Parekh J, Chanda SV. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J Biol 2007;31:53-8.

Evans WC. Trease and Evans Pharmacology. 14th ed. Singapore: Harcourt Brace and company Asia Pvt. Ltd.; 1997.

Shalini S, Sampathkumar P. Phytochemical screening and antimicrobial activity of plant extracts for disease management. Int J Curr Sci 2012;209-18.

Sharma PC, Yelne MB, Dennis TJ. Database on Medicinal Plants used in Ayurveda and Sidha. Vol. 3. New Delhi: CCRAS, Department of Ayush, Ministry of Health and Family Welfare, Government of India; 2000. p. 229.

Kokate CK. Practical Pharmcognosy. New Delhi: Vallabh Prakashan Publication; 1999. p. 111-6.

Gahan PB. Plant Histochemistry and Cytochemistry: An Introduction. Florida, USA: Academic Press; 1984. p. 1-123.

Sofowora A. Medicinal Plants and Traditional Medicinal in Africa. 2nd ed. Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd., Screening Plants for Bioactive Agents; 1993. p. 134-56.

Siddhuraju P, Becker K. The antioxidant and free radical scavenging activities of processed cowpea Vigna unguiculata L. See extracts. Food Chem 2007;101:10-9.

Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem 2001;49(7):3420-4.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem 1999;269(2):337-41.

Hatano T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects. Chem Pharm Bull (Tokyo) 1988;36(6):2090-7.

Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Bahramian F. In vitro antioxidant activity of Phytolacca americana berries. Pharmacologyonline 2009;1:81-8.

Barros L, Falcao S, Baptista P, Freire C, Vilas-Boas M, Ferreira CF. Antioxidant activity of Agaricus sp. Mushrooms by chemical, biochemical and electrochemical assays. Food Chem 2008;111:61-6.

Indrianingsih AW, Tachibanac S, Itohc K. In vitro evaluation of antioxidant and Î±-glucosidase inhibitory assay of several tropical and subtropical plants. Procedia Environ Sci 2015;28:639-48.

Pereira MD, Herdeiro RS, Fernandes PN, Eleutherio EC, Panek AD, Golla AD. Evaluation of antioxidant and DNA damage protection activity of the hydroalcoholic extract of Desmostachya bipinnata L. Stapf. Targets of oxidative stress in yeast sod mutants. Biochim.Biophys. Acta 2003;1620(1-3):245-51.

Perez-Jimenez J, Arranz S, Tabernero M. Updated methodology to determine antioxidant capacity in plant foods, oils and beverages: Extraction, measurement and expression of results. Food Res Int 2008;41(3):274-85.

Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 2004;85(2):231-7.

Harborne JB. Classes and functions of secondary products from plants. In: Walton JN, Brown DE, editors. Chemicals from Plantsdperspectives on Plant Secondary Products. London, UK: Imperial College Press; 1999. p. 1-25.

Turkmen N, Sari F, Velioglu YS. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Foline Ciocalteu

methods. Food Chem 2006;99(4):835-41.

Nakayama T, Yamada M, Osawa T, Kawakishi S. Suppression of active oxygen-induced cytotoxicity by flavonoids. Biochem Pharmacol 1993;45:265-7.

Govindarajan R, Rastogi S, Vijayakumar M, Shirwaikar A, Rawat AK, Mehrotra S, et al. Studies on the antioxidant activities of Desmodium gangeticum. Biol Pharm Bull 2003;26(10):1424-7.

Yang QM, Pan X, Kong W. Antioxidant activities of malt extract from barley Hordeum vulgare L. Toward various oxidative stress in vitro and in vivo. Food Chem 2010;118(1):84-9.

Gorinstein S, Martin-Belloso O, Katrich E, Lojek A, CÃz M, Gligelmo-Miguel N, et al. Comparison of the contents of the main biochemical compounds and the antioxidant activity of some Spanish olive oils as determined by four different radical scavenging tests. J Nutr Biochem 2003;14(3):154-9.

Fukumoto LR, Mazza G. Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem 2000;48(8):3597-604.

Golla U, Bhimathati SS. Evaluation of antioxiant and DNA damage protection activity of the hydroalcoholic extract of Desmostachya bipinnata L. Stapf. Sci World J 2014;2014:215084.