Antibacterial and anti-biofilm activities of crude extracts of Lawsonia inermis against Methicillin Resistant Staphylococcus aureus

Triveni AG; Mendem Suresh Kumar; Channapa T Shivannavar; Subhaschandra M Gaddad

doi:10.22159/ajpcr.2016.v9i6.14362

Authors

Triveni AG Research scholar,Department of Microbiology,Gulbarga University,Kalaburagi.585106
Mendem Suresh Kumar Department of Microbiology,Gulbarga University,Kalaburgi,Karnataka -585106
Channapa T Shivannavar Department of Microbiology, Gulbarga University, Kalaburgi, Karnataka -585106
Subhaschandra M Gaddad Department of Microbiology, Gulbarga University, Kalaburgi, Karnataka -585106

DOI:

https://doi.org/10.22159/ajpcr.2016.v9i6.14362

Abstract

Â

Objective:

The continuous rise in the prevalence of multi drug resistance pathogens globally is threatening the treatment and management of infectious diseases. Ethno medicine plays a key role in the exploration for novel bioactive compounds. The present study evaluates the antibacterial and antibiofilm activities of the crude extracts of Lawsonia inermis against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA).

Materials and methods: Shade dried and finely powdered leaves of the plant were extracted by maceration method using six solvents methanol, acetone, ethyl acetate, chloroform, petroleum ether and n-hexane. Antibacterial and antibiofilm activities of the extracts against MDR MRSA by agar cup diffusion and tube method respectively.

Results: Methanol extract showed the highest antibacterial activity of 18mm compared to other extracts. Similarly, petroleum ether extract showed highest biofilm inhibition of 84.7%. Other solvent extracts also exhibited significant biofilm inhibition [n-Hexane-83.6%, Ethyl acetate -79.5%, Chloroform-79.2%, Acetone -77% and Methanol-77%].

Conclusion: The leaf extracts of L. inermis have shown promising biofilm inhibitory activity and good antibacterial activity, which can be explored for the development of new drugs for the MDR pathogens.

Â

Keywords: Antibioticacivity, Antibiofilm activity, L.inermis, MRSA

Downloads

Download data is not yet available.

Author Biographies

Triveni AG, Research scholar,Department of Microbiology,Gulbarga University,Kalaburagi.585106

Research scholar,
Department of Microbiology,
Gulbarga University,
Kalaburagi.585106

Mendem Suresh Kumar, Department of Microbiology,Gulbarga University,Kalaburgi,Karnataka -585106

Research scholar,
Department of Microbiology,
Gulbarga University,
Kalaburagi.585106

Channapa T Shivannavar, Department of Microbiology, Gulbarga University, Kalaburgi, Karnataka -585106

Department of Microbiology,

Professor

Subhaschandra M Gaddad, Department of Microbiology, Gulbarga University, Kalaburgi, Karnataka -585106

Department of Microbiology,

Professor

References

References:

David, M. Z., & Daum, R. S. (2010). Community-Associated Methicillin Resistant Staphylococcus aureus: Epidemiology and Clinical Consequences of an Emerging Epidemic. Clinical Microbiology Reviews, 23(3), 616â€“687.

Mendem S K, Triveni A. G, C. T. Shivannavar, S. M. Gaddad. Antibiotic resistance patterns of Staphylococcus aureus: A multi-center study from India, Microbial Pathogenesis, Volume 98, September 2016, Pages 167-170, ISSN 0882-4010, http://dx.doi.org/10.1016/j.micpath.2016.07.010.

Al-Rubiay, K. K., Jaber, N. N., Al-Mhaawe BH, & Alrubaiy, L. K. (2008). Antimicrobial Efficacy of Henna Extracts. Oman Medical Journal, 23(4), 253â€“256.

Kidanemariam T. K, Tesema T. K, Asressu K. H, Boru A. D. Chemical Investigation of Lawsonia inermis L. Leaves from Afar Region, Ethiopia . Orient J Chem 2013;29(3)

G. U. Bautista-Trujillo,1 J. L. Solorio-Rivera,2 I. RenterÄ±Â´a-SoloÂ´rzano,2 S. I. Carranza-GermaÂ´n,2 J. A. Bustos-MartÄ±Â´nez,3 R. I. Arteaga-Garibay,4 V. M. Baizabal-Aguirre,1 M. Cajero-JuaÂ´rez,1,5 A. Bravo-PatinËœo1 and J. J. Valdez-AlarcoÂ´n. Performance of culture media for the isolation and identification of Staphylococcus aureus from bovine mastitis. Journal of Medical Microbiology (2013), 62, 369â€“376.

Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI document M100-S24, 2014. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA

Christensen GD, Simpson WA, Younger JA et al. Adherence of coagulase negative Staphylococci to plastic tissue cultures: a quantitative model for the adherence of Staphylococci to medical devices. J Clin Microbiol 1995;22:996-1006.

Jessica L. Lister and Alexander R. Horswill.Staphylococcus aureus biofilms: recent developments in biofilm dispersal. Front. Cell. Infect. Microbiol., 2014;4.

Chaudhary G, Goyal S, Poonia P. Lawsonia inermis Linnaeus: a phytopharmacological review. Int J Pharm Sci Drug Res. 2010;2(2):91-8.

Dixit SN, Srivastava HS, Tripathi RD. Lawsone, the antifungalantibiotic from the leaves of Lawsonia inermis and some aspects of its mode of action. Indian Phytopathol. 1980; 31:131-133.

Khan MM, Ali A, Jain DC, Bhakuni RS, Zaim M, Thakur RS. Occurrence of some antiviral sterols in Artemisia annua. Plant Sci. 1991; 75(2):161-165.

Iyer MR, Pal SC, Kasture VS, Kasture SB. Effect of Lawsonia inermis on memory and behavior mediated via monoamine neurotransmitters. Indian J. Pharmacol. 1998; 30(3):181-185.

Okpekon T, Yolou S, Gleye C, Roblot F, Loiseau P, Bories C, Grelllier P, Frappier F, Laurens A, Hocquemiller R. Antiparasitic activities of medicinal plants used in Ivory Coast. J Ethanopharmacol. 2004; 90(1):91-97

Arunava Kali. Antibiotics and bioactive natural products in treatment of methicillin resistant Staphylococcus aureus: A brief review. Pharmacogn Rev. 2015;9(17): 29â€“34

MiloÅ¡ NikoliÄ‡, Sava VasiÄ‡, Jelena ÄurÄ‘eviÄ‡, Olgica StefanoviÄ‡ and Ljiljana ÄŒomiÄ‡. Antibacterial and anti-biofilm activity of Ginger (Zingiber officinale (Roscoe) ethanolic extract. Kragujevac J. Sci. 36 (2014) 129-136.