PHYTOCHEMICAL SCREENING AND EVALUATION OF IN-VITRO ANTIMICROBIAL ACTIVITY OF DROSERA SPATULATA VAR BAKOENSIS- AN INDIGENOUS CARNIVOROUS PLANT AGAINST RESPIRATORY TRACT INFECTIOUS MICROBES.
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i6.14382Abstract
Objective: In the present study, the phytochemical constituents of Drosera spatulata, have been evaluated and antimicrobial activity was screened against respiratory tract infectious microbes.
Methods: The phytochemicals present in Drosera spatulata by qualitative phytochemical assays and the antimicrobial activity along with MIC, MBC and BIC were determined against S. aureus, Klebsiella pneumonia and S.Pneumonia the causative organisms of pulmonary infections, mainly effects the nasal pharynx, trachea, and lungs as well as Aspergillus niger.
Results: Aqueous, ethanol, methanol extracts of thick roots, open flower and hair of Drosera spatulata var bakoensis against this pathogenic bacteria and fungi showed high zone of inhibition which estimated by disc-diffusion method as well as minimum inhibition concentration manifestation by the broth microdilution assay followed MBC and BIC. The values of MIC, MBC and BIC obtained were between 0.3-0.9, 0.36-2.25, 0.12 - 0.37 mg/mL. The results revealed that the plant extracts of Drosera spatulata var bakoensis have high potential even at low concentrations values against bacteria and fungi cultures and this results validated by the presence of high amounts of alkaloids, quinones, anthraquinones, flavonoids in the plant extracts.
 Conclusion: In the present study, the results showed the presence of high amounts of alkaloids, flavonoids, quninoes, anthraquinones, terpinoids in Drasera spatulata plant.  In the antibacterial and antifungal activity, the ethanol and methanol extracts significantly showed the activity against the tested respiratory disease causing bacteria and antifungal properties with zone of inhibition showed more than aqueous extracts at very low concentrations
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References
Jayaram K, Prasad MN. Drosera indica L. and D. burmanii Vahl., medicinally important insectivorous plants in Andhra Pradesh-regional threats and conservation. Current science. 2006 Oct 10;91(7):943-7.
Juniper BE, Robins RJ, Joel DM. The carnivorous plants. London, etc.: Academic Press; 1989.
Albert, Victor A., Stephen E. Williams, and Mark W. Chase. "Carnivorous plants: phylogeny and structural evolution." American Association for the Advancement of Science, 1992.
Ellison AM, Gotelli NJ. Energetics and the evolution of carnivorous plants—Darwin's ‘most wonderful plants in the world’. Journal of Experimental Botany. 2009;60(1):19-42.
W.S. Barthlott, Porembski.R.Seine and I.Theison (translated by M.Ashdown). The curious world of carnivorous plants: A Comprehensive Guide to their Biology and Cultivation, Timber Press,Portland (2007).
Khare, C. P. "Indian Medicinal Plants: an illustrated dictionary Library of congress Control Number: 2007922446 ISBN: 978-0-387-70637-5 Springer-Verlag Berlin." (2007).
S. THOS, M. D. BLAIR and J. J. TAYLOR, A Practitioner's Handbook Of Materia Medica And Therapeutics Based Upon Established Physiological Actions And The Indications In Small Doses. Member American Medical Association, Pennsylvania State Medical Society, Harrisburg Academy Of Medicine, Member Visiting Staff Of Harrisburg City Hospital, &C. The Medical Council, 4105 Walnut Street, Philadelphia, (1907), Pp. 37.
N.D. Ronald Steriti, PhD, The Naturopathic Treatment Notebook, evaluated by FDA, (1997), pp. 34.
Buck JD, Cleverdon RC. The spread plate as a method for the enumeration of marine bacteria. Limnology and Oceanography. 1960 Apr 1;5(1):78-80.
Gaydos JM, Harrington BJ. Agar disk diffusion for the quality control testing of Autobac elution disks. Antimicrobial agents and chemotherapy. 1982 Mar 1;21(3):516-8.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology. 1966 Apr;45(4):493.
Rios JL, Recio MC, Villar A. Screening methods for natural products with antimicrobial activity: a review of the literature. Journal of ethnopharmacology. 1988 Aug 31;23(2):127-49.
Johnson SA, Goddard PA, Iliffe C, Timmins B, Rickard AH, Robson G, Handley PS. Comparative susceptibility of resident and transient hand bacteria to paraâ€chloroâ€metaâ€xylenol and triclosan. Journal of
applied microbiology. 2002 Aug 1;93(2):336-44.
Nostro A, Roccaro AS, Bisignano G, Marino A, Cannatelli MA, Pizzimenti FC, Cioni PL, Procopio F, Blanco AR. Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. Journal of medical microbiology. 2007 Apr 1;56(4):519-23.
Jones WP, Kinghorn AD. Extraction of plant secondary metabolites. Natural Products Isolation. 2012:341-66.
D. Schell, Medicinal uses of some American carnivorous plants. Carniv Plant Newsl (1984), 13:34-36
Sujana, Para, Thulasi Muneppa Sridhar, Penchalaneni Josthna, and Challagundla Varadarajulu Naidu. "Antibacterial activity and phytochemical analysis of Mentha piperita L.(Peppermint)—An important multipurpose medicinal plant." (2013).
Korsgaard J, Møller JK, Kilian M. Antibiotic treatment and the diagnosis of Streptococcus pneumoniae in lower respiratory tract infections in adults. International journal of infectious diseases. 2005 Sep 30;9(5):274-9.
Berendt RF, Schneider MA, Young HW, Frola FR. Protection against Klebsiella pneumoniae respiratory tract infection of mice and squirrel monkeys given kanamycin by aerosol and injection. American journal of veterinary research. 1979 Sep;40(9):1231-5.
R. George, M.D. Thompson III, F. Thomas and M.D. Patterson, Pulmonary Aspergillosis, Theme Medical Publishers, Med., 2008 Vol 28(2):pp. 103-110.
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