ANTIMICROBIAL ACTIVITY OF DIETHYL PHTHALATE – AN INSILICO APPROACH
Abstract
Reactive oxygen species (ROS)are produced by host phagocytes and can attack a diverse range of targets to exert antimicrobial activity, against
a broad range of pathogens. Four major ROS are recognized comprising superoxide, hydrogen peroxide, hydroxyl radical (•OH), and singlet
oxygen. Antioxidant enzymes such as glutathione peroxidase (GPX), superoxide dismutase and glutathione (GSH)reductase plays a crucial role
by protecting the organism from endogenous ROS diethyl phthalate is a secondary metabolite produced from endophytic fungi. Diethyl phthalate and
generally phthalate esters have an inhibitory effect on superoxide dismutase (SOD), While comparing the results from the docking experiments
and their interactions among the three enzymes SOD have the least binding energy of −6.45 kcal and more stable with the ligand diethyl phthalate
after it has been docked, anti-oxidant function of SOD is inhibited more rather than the other two proteins GPX and GSH reductase. As a result
there is an increase in superoxide production, which was found to exert ROS generated oxidative stress in the cytoplasm of bacterial cells, leading
to cell death.
Keywords: Endophytic fungi, Diethyl phthalate, Reactive oxygen species, Antioxidant enzymes.
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References
Tan RX, Zou WX. Endophytes: Arich source of functional metabolites.
Nat Prod Rep 2001;18(4):448-59.
Vining LC. Functions of secondary metabolites. Annu Rev Microbiol
;44:395-427.
Yu H, Zhang L, Li L, Zheng C, Guo L, Li W, et al. Recent developments
and future prospects of antimicrobial metabolites produced by
endophytes. Microbiol Res 2010;165(6):437-49.
Keren I, Shah D, Spoering A, Kaldalu N, Lewis K. Specialized persister
cells and the mechanism of multidrug tolerance in Escherichia coli.
J Bacteriol 2004;186(24):8172-80.
Kohanski MA, Dwyer DJ, Hayete B, Lawrence CA, Collins JJ.
A common mechanism of cellular death induced by bactericidal
antibiotics. Cell 2007;130(5):797-810.
Kohanski MA, Dwyer DJ, Wierzbowski J, Cottarel G, Collins JJ.
Mistranslation of membrane proteins and two-component
system activation trigger antibiotic-mediated cell death. Cell
;135(4):679‑90.
Fridovich I. Superoxide radical: an endogenous toxicant. Annu Rev
Pharmacol Toxicol 1983;23:239-57.
Hassan HM, Fridovich I. Intracellular production of superoxide radical
and of hydrogen peroxide by redox active compounds. Arch Biochem
Biophys 1979;196(2):385-95.
Turrens JF. Mitochondrial formation of reactive oxygen species.
J Physiol 2003;552:335-44.
Apel K, Hirt H. Reactive oxygen species: Metabolism, oxidative stress,
and signal transduction. Annu Rev Plant Biol 2004;55:373-99.
Barbehenn RV. Gut-based antioxidant enzymes in a polyphagous and a
graminivorous grasshopper. J Chem Ecol 2002;28(7):1329-47.
Page BD, Lacroix GM. The occurrence of phthalate ester and
di‑2‑ethylhexyl adipate plasticizers in Canadian packaging and food
sampled in 1985-1989: A survey. Food Addit Contam 1995;12(1):129‑51.
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