GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF METHANOLIC LEAF EXTRACT OF CASSIA ANGUSTIFOLIA VAHL.

Shahina Parveen; Anwar Shahzad; Anamica Upadhyay; Vikas Yadav

doi:10.22159/ajpcr.2016.v9s3.14512

Authors

Shahina Parveen Aligarh Muslim University, ALigarh
Anwar Shahzad
Anamica Upadhyay
Vikas Yadav

DOI:

https://doi.org/10.22159/ajpcr.2016.v9s3.14512

Abstract

ABSTRACT
Objective: The leaves of Cassia angustifolia Vahl. are employed for the treatment of several human diseases. Therefore, the present study was
undertaken to determine the phytocomponents present in the methanolic extract of the leaves by gas chromatography-mass spectrometry (GC-MS)
Methods: The collected leaf samples were dried and extracted in methanol. Screening of the extract was done by GC-MS which is an important
technique for the separation and identification of different phytochemicals.
Results: The methanolic extract of the leaves of C. angustifolia revealed the presence of 45 different phytochemicals. The prevailing compounds were
1 butanol, 3 methyl acetate (area % 7.47), 6, 6-dideutero-nonen-1-Ol-3 (area % 10.45), pentadecanoic acid (area % 9.22), and squalene (area %
12.30). Vitamin E (area % 3.85) has also been found in the leaf extract. Some of the compounds possess biological activities.
Conclusions: It can be concluded from the present study that some of the identified phytochemicals could be responsible for the medicinal value or
biological activity of the plant leaves.
Keywords: Senna, Leaf extract, Methanol, Gas chromatography-mass spectrometry, Phytochemicals, Vitamin E.

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Author Biography

Shahina Parveen, Aligarh Muslim University, ALigarh

Department of Botany

Post Doctoral Fellow

References

Mukherjee PK, Kumar V, Houghton PJ. Screening of Indian medicinal plants for acetylcholinesterase inhibitory activity. Phytother Res 2007;21(12):1142-5.

Anonymous. The Wealth of India, Raw Materials. Vol. 3. New Delhi: CSIR; 1992. p. 354-63.

Duke J. Phytochemical and Ethnobotanical Databases. U.S. Department of Agriculture, Agricultural Research Service; 1992-2016. Home Page.Available from: http://www.phytochem.nal.usda.gov/http://www.dx.doi.org/10.15482/USDA.ADC/1239279.

Smith TJ. Squalene: Potential chemopreventive agent. Expert Opin Investig Drugs 2000;9(8):1841-8.

Owen RW, Haubner R, WÃ¼rtele G, Hull E, Spiegelhalder B, Bartsch H. Olives and olive oil in cancer prevention. Eur J Cancer Prev 2004;13(4):319-26.

Smedman AE, Gustafsson IB, Berglund LG, Vessby BO. Pentadecanoic acid in serum as a marker for intake of milk fat: relations between intake of milk fat and metabolic risk factors. Am J Clin Nutr 1999;69(1):22-9.

Thomas N. Synthesis of vitamin E. In: Gerald L, editor. Vitamin E. Vitamins & Hormones. Vol. 76. San Diego: Elsevier Academic Press Inc.; 2007. p. 155-202.

Alison D, Richard P, Mark H, Andrew A. The synthesis of naturally occurring vitamin K and vitamin K analogues. Curr Org Chem 2003;7:1625-34.

McGinty D, Letizia CS, Api AM. Fragrance material review on phytol. Food Chem Toxicol 2010;48 Suppl 3:S59-63.

Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. Vol. 2. New Delhi India: Publications and Information Directorate; 1993. p. 179.

Sermakkani M, Thangapandian V. GC-MS analysis of Cassia italica leaf methanol extract. Asian J Pharm Clin Res 2012;5(2):90-4.

Ibibia ET, Olabisi KN, Oluwagbemiga OS. Gas chromatography-mass spectrometric analysis of methanolic leaf extracts of Lannea kerstingii and Nauclea diderrichii, two medicinal plants used for the treatment of gastrointestinal tract infections. Asian J Pharm Clin Res 2016;9(4):179-82.