BSMI AND TAQI POLYMORPHISMS IN VITAMIN D RECEPTOR GENE OF TYPE 2 DIABETES MELLITUS PATIENTS FROM NORTH INDIA

Authors

  • Nancy Taneja Jaypee Institute of Information Technology
  • Rajesh Khadagawat All India Institute of Medical Sciences, New Delhi
  • Shalini Mani Jaypee Institute of Information Technology, Noida

DOI:

https://doi.org/10.22159/ajpcr.2016.v9s3.14875

Abstract

ABSTRACT
Objective: Polymorphisms in vitamin D receptor (VDR) genes are known to be linked with different metabolic diseases including Type 2 diabetes
mellitus (T2DM) also. However, the association of these polymorphisms is not much explored for the Indian population. To determine the prevalence
of BsmI and TaqI polymorphism in VDR gene of T2DM patients from North India.
Methods: Blood samples were obtained from 100 well-characterized T2DM patients and 100 healthy controls. Genomic DNA was isolated from blood
samples and using polymerase chain reaction/restriction fragment length polymorphism based method, the presence of these polymorphisms was
investigated in these samples. The data were statistically analyzed using SPSS 21.0 software.
Results: For TaqI polymorphism, both the wild type (TT) and heterozygous (TC) genotype showed a significant difference between patients and
controls (p=0.023 and p<0.001, respectively). Whereas, the frequency of CC genotype was not significantly different among these groups (p=0.506).
For BsmI polymorphism also, the frequency of wild type (GG) and heterozygous (GA) genotype was significantly different in patients and controls
(p=0.027 and p=0.001), respectively. However, the frequency of AA genotype was not of statistical significance in patients (p=0.071).
Conclusions: The mutant alleles of TaqI and BsmI polymorphisms are known to be associated with different metabolic diseases, including diabetes
too. In our study also, there is a significant difference between the frequency of wild type and heterozygous genotype for these polymorphisms. This
suggests that BsmI and TaqI polymorphisms may be associated with T2DM patients.
Keywords: Type 2 diabetes mellitus, Polymorphism, Vitamin D receptor, Patient, Control, Restriction fragment length polymorphism.

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References

American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2004;27 Suppl 1:S5-10.

Kaveeshwar SA, Cornwall J. The current state of diabetes mellitus in India. Australas Med J 2014;7(1):45-8.

Chantal M, Conny G, Annapaula G, Roger B. Vitamin D and diabetes. Diabetologia 2005;48(7):1247-57.

Takiishi T, Gysemans C, Bouillon R, Mathieu C. Vitamin D and diabetes. Endocrinol Metab Clin North Am 2010;39(2):419-46.

Mitri J, Pittas AG. Vitamin D and diabetes. Endocrinol Metab Clin North Am 2014;43(1):205-32.

Palomer X, González-Clemente JM, Blanco-Vaca F, Mauricio D. Role of vitamin D in the pathogenesis of Type 2 diabetes mellitus. Diabetes Obes Metab 2008;10(3):185-97.

Venkatesh G, Jayprakash A, Swathi KS. The effect of sun light exposure on prediabetic patients in south Indian population. Int J Pharm Pharm Sci 2014;6(11):107-10.

Mallayasamy M, David HA, Pandian PR. Comparison of angiotensin receptor blocker alone and in combination with vitamin D analogue in reducing proteinuria in diabetic nephropathy patients. Int J Pharm Pharm Sci 2014;6(8):201-4.

Hossein-nezhad A, Spira A, Holick MF. Influence of vitamin D status and vitamin D3 supplementation on genome wide expression of white blood cells: A randomized double-blind clinical trial. PLoS One 2013;8(3):e58725.

Montecino M, Stein GS, Stein JL, Lian JB, van Wijnen AJ, Carvallo L, et al. Vitamin D control of gene expression: Temporal and spatial parameters for organization of the regulatory machinery. Crit RevEukaryot Gene Expr 2008;18(2):163-72.

Tarroni P, Villa I, Mrak E, Zolezzi F, Mattioli M, Gattuso C, et al. Microarray analysis of 1,25(OH)2D3 regulated gene expression in human primary osteoblasts. J Cell Biochem 2012;113(2):640-9.

McCullough ML, Stevens VL, Diver WR, Feigelson HS, Rodriguez C, Bostick RM, et al. Vitamin D pathway gene polymorphisms, diet, and risk of postmenopausal breast cancer: A nested case-control study. Breast Cancer Res 2007;9(1):R9.

Cicek MS, Liu X, Schumacher FR, Casey G, Witte JS. Vitamin D receptor genotypes/haplotypes and prostate cancer risk. Cancer Epidemiol Biomarkers Prev 2006;15(12):2549-52.

Gao L, Tao Y, Zhang L, Jin Q. Vitamin D receptor genetic polymorphisms and tuberculosis: Updated systematic review and meta-analysis. Int J Tuberc Lung Dis 2010;14(1):15-23.

Wang TJ, Zhang F, Richards JB, Kestenbaum B, van Meurs JB, Berry D, et al. Common genetic determinants of vitamin D insufficiency: A genome-wide association study. Lancet 2010;376(3736):180-8.

Györffy B, Vásárhelyi B, Krikovszky D, Madácsy L, Tordai A, Tulassay T, et al. Gender-specific association of vitamin D receptor polymorphism combinations with Type 1 diabetes mellitus. Eur J Endocrinol 2002;147(6):803-8.

Hitman GA, Mannan N, McDermott MF, Aganna E, Ogunkolade BW, Hales CN, et al. Vitamin D receptor gene polymorphisms influence insulin secretion in Bangladeshi Asians. Diabetes 1998;47(4):688-90.

Morrison NA, Qi JC, Tokita A, Kelly PJ, Crofts L, Nguyen TV, et al. Prediction of bone density from vitamin D receptor alleles. Nature 1994;367(6460):284-7.

Hustmyer FG, DeLuca HF, Peacock M. ApaI, BsmI, EcoRV and TaqI polymorphisms at the human vitamin D receptor gene locus in Caucasians, blacks and Asians. Hum Mol Genet 1993;2(4):487.

Ma J, Stampfer MJ, Gann PH, Hough HL, Giovannucci E, Kelsey KT, et al. Vitamin D receptor polymorphisms, circulating vitamin D metabolites, and risk of prostate cancer in United States physicians. Cancer Epidemiol Biomarkers Prev 1998;7(5):385-90.

Jurutka PW, Whitfield GK, Hsieh JC, Thompson PD, Haussler CA, Haussler MR. Molecular nature of the vitamin D receptor and its role in regulation of gene expression. Rev Endocr Metab Disord 2001;2(2):203-16.

Ortlepp JR, Metrikat J, Albrecht M, Von Korff A, Hanrath P, Hoffmann R. The vitamin D receptor gene variant and physical activity predicts fasting glucose levels in healthy young men. Diabet Med 2003;20(6):451-4.

Li L, Wu B, Liu JY, Yang LB. Vitamin D receptor gene polymorphisms and Type 2 diabetes: A meta-analysis. Arch Med Res 2013;44(3):235-41.

Ye WZ, Reis AF, Dubois-Laforgue D, Bellanné-Chantelot C, Timsit J, Velho G. Vitamin D receptor gene polymorphisms are associated with obesity in Type 2 diabetic subjects with early age of onset. Eur J Endocrinol 2001;145(2):181-6.

Malecki MT, Frey J, Moczulski D, Klupa T, Kozek E, Sieradzki J. Vitamin D receptor gene polymorphisms and association with Type 2 diabetes mellitus in a Polish population. Exp Clin Endocrinol Diabetes 2003;111(8):505-9.

Cyganek K, Mirkiewicz-Sieradzka B, Malecki MT, Wolkow P, Skupien J, Bobrek J, et al. Clinical risk factors and the role of VDR gene polymorphisms in diabetic retinopathy in Polish Type 2 diabetes patients. Acta Diabetol 2006;43(4):114-9.

Bid HK, Konwar R, Aggarwal CG, Gautam S, Saxena M, Nayak VL, et al. Vitamin D receptor (FokI, BsmI and TaqI) gene polymorphisms and Type 2 diabetes mellitus: A North Indian study. Indian J Med Sci 2009;63(5):187-94.

Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16(3):1215.

Acikbas I, Sanli B, Tepeli E, Ergin S, Aktan S, Bagci H. Vitamin D receptor gene polymorphisms and haplotypes (Apa I, Bsm I, Fok I, Taq I) in Turkish psoriasis patients. Med Sci Monit 2012;18(11):CR661-6.

Naito M, Miyaki K, Naito T, Zhang L, Hoshi K, Hara A, et al. Association between vitamin D receptor gene haplotypes and chronic periodontitis among Japanese men. Int J Med Sci 2007;4(4):216-22.

Li H, Miao H, Lu Y, et al. Association between DNA polymorphism of human vitamin D receptor gene and Type 2 diabetes mellitus. China J Mod Med 2005;15:989-92.

Ogunkolade BW, Boucher BJ, Prahl JM, Bustin SA, Burrin JM, Noonan K, et al. Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians. Diabetes 2002;51(7):2294-300.

Reis AF, Hauache OM, Velho G. Vitamin D endocrine system and the genetic susceptibility to diabetes, obesity and vascular disease. A review of evidence. Diabetes Metab 2005;31:318-25.

Howard G, Nguyen T, Morrison N, Watanabe T, Sambrook P, Eisman J, et al. Genetic influences on bone density: Physiological correlates of vitamin D receptor gene alleles in premenopausal women. J Clin Endocrinol Metab 1995;80(9):2800-5.

Shi H, Norman AW, Okamura WH, Sen A, Zemel MB. 1alpha,25-dihydroxyvitamin D3 modulates human adipocyte metabolism via nongenomic action. FASEB J 2001;15(14):2751-3.

Oh JY, Barrett-Connor E. Association between vitamin D receptor polymorphism and Type 2 diabetes or metabolic syndrome in community-dwelling older adults: The Rancho Bernardo study. Metabolism 2002;51(3):356-9.

Al-Daghri NM, Al-Attas OS, Alkharfy KM, Khan N, Mohammed AK, Vinodson B, et al. Association of VDR-gene variants with factors related to the metabolic syndrome, Type 2 diabetes and vitamin D deficiency. Gene 2014;542(2):129-33.

Morrison NA, Yeoman R, Kelly PJ, Eisman JA. Contribution of trans-acting factor alleles to normal physiological variability: Vitamin D receptor gene polymorphism and circulating osteocalcin. Proc Natl Acad Sci USA 1992;89(15):6665-9.

Speer G, Cseh K, Winkler G, Vargha P, Braun E, Takács I, et al. Vitamin D and estrogen receptor gene polymorphisms in Type 2 diabetes mellitus and in android type obesity. Eur J Endocrinol 2001;144(4):385-9.

Published

01-12-2016

How to Cite

Taneja, N., R. Khadagawat, and S. Mani. “BSMI AND TAQI POLYMORPHISMS IN VITAMIN D RECEPTOR GENE OF TYPE 2 DIABETES MELLITUS PATIENTS FROM NORTH INDIA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 9, Dec. 2016, pp. 186-9, doi:10.22159/ajpcr.2016.v9s3.14875.

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