EVALUTION OF IN VITRO ANTI-ANGIOGENESIS ACTIVITY ON METHANOLIC EXTRACT OF Clematis buchaniana PLANT
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i2.15348Abstract
Objective: Angiogenesis plays an important role in embryonic development and various physiological processes. However, excessive angiogenesis is associated with several pathological conditions including cancer. Clematis is a genus of about 300 species within the buttercup family Ranunculaceae. It is native to the India, where has been used as folk medicine for the treatment of various ailments. This work is aimed to evaluate the antiangiogenesis activity in the crude methanolic extract of Clematis buchaniana plant.
Methods: The entire aerial parts of C. buchaniana were extracted by soxhelation in methanol. Then, the solvent was evaporated to dryness to yield the dried crude extract of C. buchaniana. Then, the extract was subjected to preliminary phytochemical screening to determine the active constituents for effective pharmacological activity. The in-vitro antiangiogenesis effects were later evaluated using chorioallantoic membrane model carried out by incubation in fresh chicken's eggs.
Results: The crude methanolic extract of C. buchaniana was found to have slight ability to inhibit angiogenesis that was evaluated by visualization.
Conclusion: C. buchaniana plant extract inhibits angiogenesis by blocking normal vascularization in chick embryo. The ability of inhibiting angiogenic process in eggs by this extract can provide us an herbal anticancer agent in future for further scrutiny.
Keywords: Antiangiogenesis, Chorioallantoic membrane, Incubation, Angiogenesis, Clematis buchaniana, Methanolic extract.
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