PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF COMMIPHORA MUKUL FOR ANTIDEPRESSANT ACTIVITY IN ALBINO MICE
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i1.15481Abstract
Objective: The main aim and objective of the present study is to investigate the effect of Commiphora mukul (Family: Burseraceae), on depression in mice using tail suspension test (TST) and forced swim test (FST).
Methods: The oleo-gum resin of guggul was extracted with alcohol and fractionated with ethyl acetate and petroleum ether. All the fractions were subjected for preliminary phytochemical screening, using various qualitative tests. Till date, no scientific data were available on the antidepressant activity of this plant. So, in the present investigation, TST and FST are selected as animal models for evaluation of antidepressant activity in albino mice.
Results: The preliminary phytochemical screening of guggul has revealed the presence of carbohydrates, proteins, tannins, and flavonoids in hydroalcoholic fraction. Ethyl acetate fraction showed positive results toward flavonoids, alkaloids, proteins, and steroids. Hydroalcoholic, ethyl acetate, and petroleum ether fractions (50 and 100 mg/kg p.o.) of guggul administered orally for 14 successive days had decreased the immobility periods significantly in a dose-dependent manner in both TST and FST, showing significant antidepressant-like activity. The activities of the fractions were found to be comparable to imipramine in both FST and TST.
Conclusions: Although a number of synthetic drugs are being used as standard treatment for clinically depressed patient, they have adverse effects that can compromise the therapeutic treatment. In the traditional systems of medicine, many plants and formulations have been used to treat depression for thousands of years. The results of this study indicate the potential for the use of guggul as an adjuvant in the treatment of depression.
Keywords: Guggul, Commiphora mukul, Antidepressant activity, Forced swim test, Tail suspension test, Depression.
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References
Rang HP, Dale MM, Ritter JM. Pharmacology. 5th ed. Edinburgh: Churchill Livingstone; 2006. p. 535.
Richard HA, Pamella CC, Richad F, Luigi CX, Michelle CA. Lippincott’s Illustrated Reviews: Pharmacology. 4th ed. Baltimore, MD: Lippincott Williams and Wilkins; 2012. p. 142.
Dhingra D, Sharma A. A review on antidepressant plants. Nat Prod Radiance 2005;5(2):144-52.
Satyavati GV. Guggulipid: A promising hypolipidaemic agent from gum Guggul (Commiphora wightii). Econ Med Plant Res 1991;5:47-82.
Satyavati GV, Dwarakanath C, Tripathi SN. Experimental studies on the hypocholesterolemic effect of Commiphora mukul. Engl. (Guggul). Indian J Med Res 1969;57(10):1950-62.
Chaudhary G. Pharmacological properties of Commiphora wightii - An overview. Int J Pharm Pharm Sci 2012;4(3):73-5.
Anurekha J, Gupta VB. Chemistry and pharmacological profile of Guggul - A review. Indian J Tradit Knowl 2006;5(4):478-83.
Saxena VK, Sharma RN. Constituents of the essential oil from Commiphora mukul Gum resin. J Med Arom Plant Sci 1998;20:55-6.
Shah R, Gulati V, Palombo EA. Pharmacological properties of guggulsterones, the major active components of gum guggul. Phytother Res 2012;26(11):1594-605.
Sharma B, Salunke R, Srivastava S, Majumder C, Roy P. Effects of guggulsterone isolated from Commiphora mukul in high fat diet induced diabetic rats. Food Chem Toxicol 2009;47(10):2631-9.
Siddiqui MZ. Guggul: An excellent herbal panacea. Asian J Pharm Health Sci 2011;1:35-39.
Singh BB, Mishra LC, Vinjamury SP, Aquilina N, Singh VJ, Shepard N. The effectiveness of Commiphora mukul for osteoarthritis of the knee: An outcomes study. Altern Ther Health Med 2003;9:74-9.
Sosa S, Della TA, Loggia R, Bombardelli E. Anti-inflammatory activity of Commiphora mukul extracts. Pharmacol Res 1993;27:89-90.
Harborne JB. Phytochemical Methods - A Guide to Modern Techniques of Plant Analysis. 2nd ed. New York: Chapman and Hall; 1984. p. 1-10.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 12th ed. Pune, India: Nirali Publication; 1999. p. 156-8.
Steru L, Chermat R, Thierry B, Simon P. The tail suspension test: A new method for screening antidepressants in mice. Psychopharmacology (Berl) 1985;85(3):367-70.
Thierry B, Stéru L, Simon P, Porsolt RD. The tail suspension test: Ethical considerations. Psychopharmacology (Berl) 1986;90(2):284-5.
Porsolt RD, Bertin A, Jalfre M. Behavioral despair in mice: A primary screening test for antidepressants. Arch Int Pharmacodyn Ther 1977;229(2):327-36.
Stahl SM. Essential Psychopharmacology: Neuroscientific Basis and Practical; Applications. Cambridge: Cambridge Univertsity Press; 1998. p. 395.
Santhosh P, Venugopal R, Nilakash S, Kunjbihari S, Mangala L, Anti-depressant activity of methanolic extract of Passiflora foetida leaves in mice. Int J Pharm Pharm Sci 2011;3(1):112-5.
Sembulingam K, Sembulingam P, Namasivayam A. Effect of Ocimum sanctum Linn on noise induced changes in plasma corticosterone level. Indian J Physiol Pharmacol 1997;41(2):139-43.
Umadevi P, Murugan S, Jennifer S, Subakanmani S. Evaluation of antidepressant like activity of Cucurbita pepo seed extracts in rats. Int J Curr Pharm Res 2010;3(1):108-13.
Ishola IS, Chatterjee M, Tota S, Tadigopulla N, Adeyemi NO, Palit G et al Antidepressant and anxiolytic effects of amentoflavone isolated from Cnestis ferruginea in mice. Pharmacol Biochem Behav 2012;103:322-31.
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