IN-VITRO HYPOGLYCEMIC EVALUATION OF FRACTIONS OF HYDRO-ACOHOLIC EXTRACT OF HEARTWOOD OF TECOMELLA UNDULATA LINN.
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i2.15539Abstract
Objective: To screen α-amylase and α-glucosidase inhibitors from the different fractions of crude hydro-alcoholic extract of heartwood of Tecomella undulata Linn.
Methods: Four fractions of crude hydro-alcoholic extract of heartwood of plant were used for in-vitro inhibitory assays against digestive enzymes: α-amylase and α-glucosidase. For assay, different concentrations (20, 40, 60, 80, 100 µg/ml) were used for all fractions. Standard protocol was used for preliminary phytochemical screening of different bioactive components present in all fractions.
Results: The fractions have shown moderate to highest inhibitory activity against both enzymes. But, the strong inhibition was revealed by acetone fraction against α-amylase with very minimal inhibitory concentrations at IC50 values when compared with a standard drug acarbose. Several medicinally active phytocomponents such as flavanoids, saponin, anthraquinones, tannins, triterpenoids and phenols were observed in all studied fractions.
Conclusion: The different fractions prepared from crude hydro-alcoholic extract of heartwood of plant are capable of inhibiting α-amylase and α-glucosidase and it can be concluded that heartwood of Tecomella undulata Linn. is partially active against postprandial hyperglycemia, thus diabetes mellitus.
Keywords: Tecomella undulata Linn., Diabetes mellitus, α-Amylase, α-Glucosidase.
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Olokoba AB, Obateru OA, Olokoba LB. Type 2 diabetes mellitus: A review of current trends. Oman Med J 2012;27(4):269-73.
Chen ZJ, Yuan P, Ye XP. α-Glucosidase and α-amylase inhibitory activities from 41 herbs for diabetes. Chin Tradit Pat Med 2008;30(11):1661-4.
Xiao-Ping YE, Chun-Qing S, Ping Y, Ren-Gang M. α-Glucosidase and α-amylase inhibitory activity of common constituents from traditional Chinese medicine used for diabetes mellitus. Chin J Nat Med 2010;8(5):0349-52.
International Diabetes Federation. IDF Diabetes Atlas. 4th ed. Brussels: International Diabetes Federation; 2009.
International Diabetes Federation. IDF Diabetes Atlas. 7th ed. 2015. Available from: http://www.idf.org/idf-diabetes-atlas-seventh-edition.
Unnikrishnan R, Anjana RM, Mohan V. Diabetes in South Asians: Is the phenotype different? Diabetes 2014;63(1):53-5.
Ali H, Houghton PJ, Soumyanath A. Alpha-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 2006;107(3):449-55.
Sales PM, Souza PM, Simeoni LA, Silveira D. Alpha-Amylase inhibitors: A review of raw material and isolated compounds from plant source. J Pharm Pharm Sci 2012;15(1):141-83.
Kim KY, Nguyen TH, Kurihara H, Kim SM.Alpha-glucosidase inhibitory activity of bromophenol purified from the red alga Polyopes lancifolia. J Food Sci 2010;75(5):H145-50.
Dineshkumar B, Analava M, Manjunatha M. A comparative study of alpha amylase inhibitory activities of common antidiabetic plants of Kharagpur 1 block. Int J Green Pharm 2010;4:115-21.
Tripathi BK, Srivastava AK. Diabetes mellitus: Complications and therapeutics. Med Sci Monit 2006;12:RA130-47.
Yao X, Zhu L, Chen Y, Tian J, Wang Y. In vivo and in vitro antioxidant activity and a-glucosidase, a-amylase inhibitory effects of flavonoids from Cichorium glandulosum seeds. Food Chem 2013;139(1-4):59-66.
Mojica L, Meyer A, Mark A, de MejÃa EG. Bean cultivars (Phaseolus vulgaris L.) have similar high antioxidant capacity, in vitro inhibition of α-amylase and α-glucosidase while diverse phenolic composition and concentration. Food Res Int 2015;69:38-48.
Khan SA, Al Kiyumi AR, Al Sheidi MS, Al Khusaibi TS, Al Shehhi NM, Alam T. In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L. Asian Pac J Trop Biomed 2016;6(4):322-9.
Wang Y, Yang Z, Wei X. Sugar compositions, a-glucosidase inhibitory and amylase inhibitory activities of polysaccharides from leaves and flowers of Camellia sinensis obtained by different extraction methods. Int J Biol Macromol. 2010 1;47:534-9.
Khatri A, Garg A, Agrawal SS. Evaluation of hepatoprotective activity of aerial parts of Tephrosia purpurea L. and stem bark of Tecomella undulata. J Ethnopharmacol 2009;122:1-5.
Khare CP. Indian Herbal Remedies, Rational Western Therapy, Ayurvedic and Other Traditional Usage, Botany. New Delhi, India: Society of New Age Herbals; 2004. p. 67.
Ambasta SP. The Useful Plants of India. New Delhi: National Institute of Science Communication and Information Resources; 2000. p. 623.
Nagpal N, Arora M, Rahar S. Pharmacological and phytochemical review on Tecomella undulata. Res J Pharmacogn Phytochem
;2:354.
Mohibbe Azam M, Ghanim A. Flavones from leaves of Tecomella undulata (Bignoniaceae). Biochem Syst Ecol 2000;28:803-4.
Bagheel SS, Dangi S, Soni P. Acute toxicity study of aqueous extract of Coccinia indica (Roots). Asia J Res Pharm Sci 2011;1:23-5.
Almeida RN, Navarro DS, Barbosa-Filho JM. Plants with central analgesic activity. Phytomedicine 2001;8:310-22.
Jain A, Katewa SS, Chaudhary BL, Galav P. Folk herbal medicines used in birth control and sexual diseases by tribals of southern Rajasthan, India. J Ethnopharmacol 2004;90(1):171-7.
Khatri A, Garg A, Agrawal SS. Evaluation of hepatoprotective activity of aerial parts of Tephrosia purpurea L. and stem bark of Tecomella undulata. J Ethnopharmacol 2009;122(1):1-5.
Danya U, Udhayasankar MR, Arumugasamy K, Baluprakash T.
Bioactivity of Tecomella undulata (G.Don) seem (Bignoniaceae) on human pathogens. South Asian J Biol Sci 2012;2(2):71-82.
Bailey CJ, Day C. Traditional plant medicines as treatments for diabetes. Diabetes Care 1989;12(8):553-64.
Kumar S, Sharma S, Vasudeva N, Ranga V. In vivo anti-hyperglycemic and antioxidant potentials of ethanolic extract from Tecomella undulata. Diabetol Metab Syndr 2012;4(1):33.
Trease GE, Evans WC. Pharmacognosy. 11th ed. London: Bailliere Tindall; 1998. p. 45-50.
Harborne JB. Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 1st ed. London: Chapman and Hall; 1998. p. 182-90.
Apostolidis E, Kwon YI, Shetty K. Inhibitory potential of herb, fruit and fungal enriched cheese against key enzymes linked to type-2 diabetes and hypertension. Innov Food Sci Emerg Technol 2007;8(1):46-54.
Subramanian R, Asmawi MZ, Sadikun A. In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta Biochim Pol 2008;55:391-8.
Rajini J, Piyush J, Poorva J. A review on treatment and prevention of diabetes mellitus. Int J Curr Pharm Res 2016;8:16-8.
Ozara TM, Bagher L, Mohammad A. A systematic review of in-vitro studies conducted on effect of herbal products on secretion of insulin from Langerhans islets. J Pharm Pharm Sci 2012;15:447-66.
Gayathri GA, Gayathri M. Preliminary qualitative phytochemical screening and in-vitro hypoglycemic potential of Acanthus ilicifolius and Evolvulus emerginatus. Int J Pharm Pharm Sci 2014;6:362-5.
Tamil IG, Dineshkumar B, Nandhakumar M, Senthilkumar M, Mitra A. In vitro study on alpha-amylase inhibitory activity of an Indian medicinal plant, Phyllanthus amarus. Indian J Pharmacol 2010;42:280-2.
McDougall GJ, Stewart D. The inhibitory effects of berry polyphenols on digestive enzymes. Biofactors 2005;23:189-95.
Miliauskas G, Yenkutonis PR, Vanbeek TA. Screening of radical scavenging activity of some medicinal and aromatic plants extracts. Food Chem 2004;85:231-7.
Kim HY, Moon BH, Lee HJ, Choi DH. Flavonol glycosides from the leaves of Eucommia ulmoides O. with glycation inhibitory activity. J Ethnopharmacol 2004;93(2-3):227-30.
Atkinson RM, Parsons BJ, Smyth DH. The intestinal absorption of glucose. J Physiol 1957;135:581-9.
Søgaard M, Abe JI, Martin-Eauclaire MF, Svensson B. α-Amylases: Structure and function. Carbohydr Polym 1993;21:137-46.
Mohan S, Pinto BM. Zwitterionic glycosidase inhibitors: Salacinol and related analogues. Carbohydr Res 2007;342:1551-80.
Kavimani S, Saminathan K, Kumar S. In-vitro antidiabetic activity of Dolichandrone atrovirens – An Indian medicinal plant. Int J Pharmacother 2014;4 Suppl 3:107-13.
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