EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i2.15570Abstract
Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum. Â
Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.
Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.
Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.
Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents
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References
Halliwell B. Free radicals, antioxidants, and human disease: Curiosity, cause, or consequence? Lancet 1994;344(8924):721-4.
Kühnau J. The flavonoids. A class of semi-essential food components: Their role in human nutrition. World Rev Nutr Diet 1976;24:117-91.
Younes M, Siegers CP. Inhibitory action of some flavonoids on enhanced spontaneous lipid peroxidation following glutathione depletion. Planta Med 1981;43(3):240-4.
Halliwell B Gutteridge JM. Oxygen toxicity, oxygen radicals, transition metals and diseases. Biochem J 1984;219:1-4.
Nunez V, Castro V, Nurillo R, Ponce-soto, LA, Merfoot I, Lomonte B.Inhibitory effects of Piper umbellatum and Piper peltatum extracts towards myotoxic phospholipase A2 from Bothrops snake venoms: Isolation of 4-nerolidylcatchol as active principle. Phytochemistry
;66:1017-25.
Dyer LA, Palmer AN. Piper: A Model Genus for Studies of Evolution, Clinical Ecology and Trophic Interactions. Boston: Kluwer Academic Publishers; 2004.
Ahmad FB, Cheksum T. Phytochemical studies on Piper umbellatum L. Asean Rev Biodivers Environ Conserv 2002;8:1-4.
Lister E, Wilson P. Measurement of Total Phenolics and ABTS Assay for Antioxidant Activity (Personal Communication). New Zealand: Crop Research Institute, Lincoln; 2001.
Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol
;72(1-2):35-42.
Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 1992;40:945-8.
Oyaizu M. Studies on products of browning reactions: Antioxidant activities of products of browning reaction prepared from glucose amine. Jpn J Nutr 1986;44:307-15.
Gryglewski RJ, Korbut R, Robak J. On the mechanism of antithrombotic action of flavonoids. Biochem Pharmacol 1987;36:317-21.
Suja S, Mohanasundari L. Antioxidant and free radical scavenging activity of the mixture of ethanolic extracts of Alpinia speciosa and Alpinia calcarata rhizome. Int J Pharm Pharm Sci 2016;8(8):164-70.
Nwauzoma AB, Songo LD. Study on the phytochemical properties and proximate analysis of Piper umbellatum (Linn) from Nigeria. Am J Res Commun 2013;1(7):164-77.
Santhy KS, Archana D, Dixitha M. Antioxidant and anti lastogenic potential of Piper longum L. Int J App Pharm 2015;7(2):11-4.
Yamaguchi T, Takamura H, Matoba T, Terao J. HPLC method for evaluation of the free radical scavenging activity of foods by using 1, 1-diphenyl-2-picrylhydrazyl. Biosci Biotech Biochem
;62:1201-4.
Tripathi YB, Chaurasia S, Tripathi E, Upadhyay A, Dubey GP. Bacopa monniera Linn. as an antioxidant: Mechanism of action. Indian J Exp Biol 1996;34:523-6.
Irulandi K, Geetha S, Mehalingam P. Evaluation of antioxidant properties and antimicrobial activity of Garcinia cambogia Hort. Ex Boerl (Clusiaceae) fruit extracts. J Adv Appl Sci 2016;1(3):74-83.
Gulcin I, Oktay M, Kırecci E, Kufrevio I. Screening of antioxidant and antimicrobial activities of anise (Pimpella anisum L.) seed extracts. Food Chem 2003;83:371-82.
Irulandi K, Mehalingam P. In vitro antioxidant and antimicrobial properties of Baccaurea courtallensis (Wight) Mull.-Arg. (Euphorbiaceae) fruit extracts. J Outreach 2015:7;64-75.
Siddhuraju P Mohan PS, Becker K. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): A preliminary assessment of crude extracts from stem bark, leaves. Flowers and fruit pulp. Food Chem 2002;79:61-70.
Agbor GA, Vinson JA, Oben JE, Ngogang JY. In vitro antioxidant activity of three Piper species. J Herb Pharmacother 2007;7(2):49-64.
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