THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i2.15724Abstract
Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).
Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.
Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.
Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.
Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.
Downloads
References
Soejono. Host Parasite Tea Tree Inventory in Purwodadi Botanical Garden. Seminar Kelompok Kerja Nasional Tumbuhan Obat Indonesia IX 21-22 September, 1995. Indonesia: Universitas Gadjah Mada; 1995.
Ohashi K, Winarno H, Mukai M, Inoue M, Prana MS, Simanjuntak P, et al. Indonesian medicinal plants. XXV. Cancer cell invasion inhibitory effects of chemical constituents in the parasitic plant Scurrula atropurpurea (Loranthaceae). Chem Pharm Bull (Tokyo)
;51(3):343-5.
Kwanda N, Noikotr K, Sudmoon R, Tanee T, Chaveerach A. Medicinal parasitic plants on diverse hosts with their usages and barcodes. J Nat Med 2013;67(3):438-45.
Tripathi S, Ray S, Das PK, Mondal AK, Verma NK. Antimicrobial activities of some rare aerial hemi parasitic taxa of South West Bengal,
India. Int J Phytopharmacol 2013;4(2):106-12.
Pandya N. Facts & comparisons 4.0. J Med Libr Assoc 2007;95(2):217-9.
Lim YC, Rajabalaya R, Lee SH, Tennakoon KU, Le QV, Idris A, et al.
Parasitic mistletoes of the genera Scurrula and Viscum: From bench to bedside. Molecules 2016;21(8). pii: E1048.
Poumorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity phenol and flavonoud contents of some selected Iranian medical plants. Afr J Biotechnol 2006;5(11):1142-5.
Dalimartha S. Atlas Indonesian Medicinal Plants. Jilid 1. Jakarta: Trubus Agriwidya; 1999. p. 146-9.
Windari FI, Rahajoe JS. Diversity of species of parasites on the Island of Java. Indonesian medicinal plants news. J Indones Med Plant
;4(4):25-9.
Litz RE. The Mango: Botany, Production and Uses. 2nd ed. Cambridge, MA: CABI; 2009. p. 269.
Mustarichie R, Indriyati W, Mukmin A, Ramdhani D. Activity of Angiopteris evecta for baldness treatment. J Chem Pharm Res
;8(5):821-30.
Gatbonton GL, De Jesus AP, Lorenzo KM, Uy MM. Soxhlet Extraction of Philippine Avocadro Fruit Pulp Variety 240, Presented at the Research Congress 2013 De La Salle University Manila March 7-9;
Marnoto T, Haryono G, Gustinah D, Putra FA. The extraction of tannins as natural dyes from plants putrimalu (Mimosa pudica) using an organic solvent. Reaktor 2012;14(1):39-45.
Departement Kesehatan RI. Indonesian Herbal Pharmacopea, Edisi I.
Jakarta: Departement Kesehtaan RI; 2015. p. 17-8.
Farnsworth NR. Biological and phytochemical screening of plant.
J Pharm Sci 1966;55(3):243-69.
Kumar S, Jyotirmayee K, Sarangi M. Thin layer chromatography: A tool of biotechnology for isolation of bioactive compounds from medicinal plants. Int J Pharm Sci Rev Res 2013;18(1):126-32.
Mustarichie R, Udin Z, Muhtadi A, Surahman E, Subarnas A, Supriyatna S. The antioxidant activity and cytotoxicity methanol extracts from cranberry plants. Int Res J Pharm Appl Sci 2012;2(4):56-61.
Garcia EJ, Oldoni TL, Alencar SM, Reis A, Loguercio AD, Grande RH.
Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth. Braz Dent J 2012;23(1):22-7.
Departement Kesehatan RI. Indonesian Herbal Pharmacopea. Jakarta:
Departement Kesehatan RI; 2008.
Shen CC, Chang YS, Hott LK. Nuclear magnetic resonance studies of
, 7-dihydroxyflavonoids. Phytochemistry 1993;34:843-5.
Shibuya H, Ohashi K, Kitagawa I. Search for pharmacological leads from tropical rainforest plants. Pure Appl Chem 1999;71(6):1109-13.
Ishizu T, Tsujino E, Winarno H, Ohashi K, Shibuya H. A complex of perseitol and K+ ion from Scurrula fusca (Loranthaceae). Tetrahedron Lett 2001;42(39):6887-9.
Lohézic-Le Dévéhat F, Tomasi S, Fontanel D, Boustie J. Flavonols from Scurrula ferruginea danser (Loranthaceae). Z Naturforsch C
;57(11-12):1092-5.
Priyanto JA, Pujiyanto S, Rukmi I. Flavonoids production capability test of mistletoe tea (Scurrula atropurpurea BL. Dans) endophytic bacteria isolates. J Sainsdan Mat 2014;22(4):89-96.
Kähkönen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, et al. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 1999;47:3954-62.
Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 2004;74:2157-84.
Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 2010;15:7313-52.
Marvibaigi M,Amini N, Supriyanto E, Jamil S, Majid FA, Jaganathan SK, et al. Total phenolic content, antioxidant and antibacterial properties of Scurrula ferruginea extracts. J Teknol 2014;5:65-72.
Krisnawati M. Isolation and identification of flavonoid compounds from parasite tea leaves (Scurrula atropurpurea Dans); 2010. Available from: http://www.zonaresearch.blogspot.co.id/2010/09/isolasi-dan- identifikasi-senyawa.html.
Jun M, Fu HY, Hong J, Wan X, Yang CS, Ho CT. Comparison of antioxidant activities of isoflavones from kudzu root (Pueraria labata Ohwi). J Food Sci Technol 2003;68(6):2117-22.
Shahidi F. Natural Antioxidant: Chemistry, Health Effects, and
Applications. USA: AOCS; 1997.
Suhartono E, Setiawan B. Oxidative stress and antioxidant role in diabetes mellitus. Majalah Kedokt Indones 2005;55(2):86.
Athiroh NA, Sulistyowati E. Scurrula atropurpurea increases nitric oxide and decreases malondialdehyde in hypertensive rats. Univ Med
;32(1):44-50.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
