Antibacterial Evaluation and Phytochemical Screening of eucalyptus gomphocephala DC against Pseudomonas aeruginosa
Abstract
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 Objective: The aim was to evaluate the in vitro antibacterial activities of essential oil and extracts from Eucalyptus gomphocephala leaf on the growth of six strains of Pseudomonas aeruginosa.
Methods: Aqueous, ethanol, hexane, petroleum ether extracts and essential oil of E. gomphocephala leaf were investigated on six strains of P. aeruginosa. The antibacterial activity was determined by agar-well diffusion method and expressed as the average diameter of the zone of inhibition of bacterial growth around the wells. The minimum inhibitory concentration (MIC) of active extracts was determined by microdilution assay. The preliminary phytochemical screening of the leaf was conducted using various standard methods.
Results: The present study reveals that the essential oil does not show any activity against all strains. Extracts showed varying degrees of inhibition on the tested microorganisms. The petroleum ether and ethanol had a lower activity against strains. However, aqueous and hexane leaf extracts, of E. gomphocephala presented the highest anti P. aeruginosa activity against all strains tested. The MIC and minimum bactericidal concentration of the aqueous and hexane extracts ranged between 6.25 and 50 mg/ml. Qualitative analysis of E. gomphocephala revealed the presence of biological active compounds such as tannins, flavonoids, saponins and steroids/triterpenes.
Conclusion: To conclude, extracts of E. gomphocephala leaf may be pursued as an antibacterial agent for the treatment of infections caused by P. aeruginosa.
Keywords: Eucalyptus gomphocephala, Extracts, Antibacterial activity, Pseudomonas aeruginosa, Phytochemical screening
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Okusa NP. Etude phytochimique et activité antimicrobienne directe et indirecte de Cordia gilletii De Wild (Boraginaceae). Thèse en Sciences biomédicales et pharmaceutiques. Faculté de pharmacie, Université libre de Bruxelles; 2012.
Kambu K. Eléments de Phytothérapie Comparée: Plantes Médicinales Africaines. Kinshasa: Centre de Recherche Pédagogique de Kinshasa; 1990. p. 148.
Takahashi T, Kokubo R, Sakaino M. Antimicrobial activities of Eucalyptus leaf extracts and flavonoids from Eucalyptus maculata. Lett Appl Microbiol 2004;39(1):60-4.
Gilles M, Zhao J, An M, Agboola S. Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species. Food Chem 2010;119:731-7.
Williams LR, Stockley JK, Yan W, Home VN. Essential oils with high antimicrobial activity for therapeutic use. Int J Aromather 1998;8:30 40.
Silva J, Abebe W, Sousa SM, Duarte VG, Machado MI, Matos FJ. Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. J Ethnopharmacol 2003;89(2-3):277-83.
Gray AM, Flatt PR. Antihyperglycemic actions of Eucalyptus globulus (Eucalyptus) are associated with pancreatic and extra-pancreatic effects in mice. J Nutr 1998;128(12):2319-23.
Guendouz BA, Doumandji B. Effect of plant extracts of Eucalyptus gomphocephala, Schinus molle and Melia azedarach on Schistocerca gregaria (Forskal, 1775) (Insectaortoptera) under laboratory conditions. Ninth Arab Congress of Plant Protection, 19-23 November 2006, Damascus, Syria.
Barbouche N, Jeridi S, Ben Dhaou S. Alternatives de lutte biologique contre Sitophilus oryzae par l’utilisation des huiles essentielles et des poudres de plantes. Rev INAT 2007;22:85-94.
Al-Sayed E, Martiskainen O, Bobrowska-Hägerstrand M, Sinkkonen J, Törnquist K, Pihlaja K, et al. Phenolic compounds from Eucalyptus gomphocephala with potential cytotoxic and antioxidant activities. Nat Prod Commun 2010;5(10):1639-42.
Sing AK, Monika K, Sushil K. Non-volatile constituents of Eucalyptus: A review on chemistry and biological activities. J Med Aromat Plant Sci 1999;21:375-407.
Benayache S, Benayache F, Benyahia S, Chalchat JC, Garry RP. Leaf oils of some Eucalyptus species growing in Algeria. J Essent Oil Res 2001;13:210-3.
Hillis WE. Polyphenols in the leaves of Eucalyptus: A chemotaxonomic survey V. The series cornutae and subcorntae of the section macrantherae and the section platyantherae. Phytochemistry 1967;6:845-56.
Al-Sayed E, Abdel-Nasser S, Nahla A, Olli M, Jari S, Kalevi P. HPLC–PDA–ESI–MS/MS profiling and chemopreventive potential of Eucalyptus gomphocephala DC. Food Chem 2012;133:1017-24.
Hajji FA, IL Idrissi S, Tetouani F, Bellakhdar J. Etude des compositions chimiques de quelques espèces d’Eucalyptus du Maroc. Al Biruniya. Rev Mar Pharm 1989;5(2):125.
Elaissi A, Rouis Z, Mabrouk S, Salah KB, Aouni M, Khouja ML, et al. Correlation between chemical composition and antibacterial activity of essential oils from fifteen Eucalyptus species growing in the Korbous and Jbel Abderrahman arboreta (North East Tunisia). Molecules 2012;17(3):3044-57.
Pranay J, Shekhar Ni, Gaurav K. Antimicrobial activity and phytochemical analysis of Eucalyptus tereticornis bark and leaf methanolic extracts. Int J Pharm Sci Rev Res 2010;4(2):126-8.
Oussou KR, Kanko C, Guessend N, Yolou S, Koukoua GM, N’guessan YT, et al. Activités antibactériennes des huiles essentielles de trois plantes de Côte d’Ivoire. C R Chimie 2004;7(10-11):1081-6.
Bruneton J. Pharmacognosie Phytochimie Plantes Médicinales. 4ème éd. Paris: Tec & Doc; 2009.
Sofowora EA. Medicinal Plants and Traditional Medicinal in Africa. Chichester: John Wiley and Sons; 1982. p. 256.
Picman AK, Schneinder EF, Picman J. Effect of flavonoids on mycelial growth of Verticillium albo-atrum. Biochem Syst Ecol 1995;23:683-93.
McGaw LJ, Rabe T, Sparg SG, Jäger AK, Eloff JN, van Staden J. An investigation on the biological activity of Combretum species. J Ethnopharmacol 2001;75(1):45-50.
Vincken JP, Heng L, de Groot A, Gruppen H. Saponins, classification and occurrence in the plant kingdom. Phytochemistry 2007;68(3):275 97.
Jayaraman P, Sakharkar MK, Lim CS, Tang TH, Sakharkar KR. Activity and interactions of antibiotic and phytochemical combinations against Pseudomonas aeruginosa in vitro. Int J Biol Sci 2010;6(6):556-68.
Jayaraman P, Sakharkar KR, Sing LC, Chow VT, Sakharkar MK. Insights into antifolate activity of phytochemicals against Pseudomonas aeruginosa. J Drug Target 2011;19(3):179-88.
Paris M, Hurabielle M. Abrégé de Matière Médicale, Pharmacognosie. Tome I. ed. Paris: Masson; 1981.
Venkatesh KV, Girish Kumar K, Pradeepa K, Santosh Kumar SR. Antibacterial activity of ethanol extract of musa paradisiacal CV. Puttabale and musa acuminate CV. Grand naine. Asian J Pharm Clin Res 2013;6:169-72.
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