ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF ETHYL ACETATE EXTRACT OF THE INDIAN GREEN MUSSEL PERNA VIRIDIS
Keywords:
: P viridis, antioxidant activity, DPPH, cytotoxic activity, DLA, EAC, MTT assayAbstract
Objective: Invertebrates are the most explored groups in the field of cytotoxic/ anti-cancer marine natural products research, considering their ecological and evolutionary significance. Hence an attempt has been done to evaluate the possible antioxidant and cytotoxic activities of the Indian green mussel Perna viridis. Methods: Qualitative analysis of the secondary metabolites present in the ethyl acetate extract of P. viridis was done using general detection reagents and spray reagents on TLC. Antioxidant activity of the same was assessed by DPPH radical scavenging assay. Brine shrimp, Dalton's Lymphoma Ascites (DLA) and Ehrlich Ascites Carcinoma (EAC) were employed for evaluating cytotoxicity of the extract. MTT assay of the extract was performed with two human cancer cell lines namely, HCT 116 and MCF 7. Results: General detection reagents and spray reagents showed the presence of different secondary metabolites in the ethyl acetate extract of P. viridis. The extract showed good scavenging activity against DPPH radical (IC50 0.616 mg/ml). It also proved significantly cytotoxic towards brine shrimp, DLA and EAC cells. MTT assay on human cell lines proved to be more cytotoxic towards MCF 7 than for HCF 116 for the extract. Conclusion: The results shows that ethyl acetate extract of P. viridis possess both antioxidant and cytotoxic activities, which can be attributed to the presence of secondary metabolites like phenolics and alkaloids
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