Glycyrrhiza glabra, disc diffusion, zone of inhibition, anti mycotic activity


Objective: The aim of the present study was to assess the anti fungal activity of ethanolic extract of Glycyrrhiza glabra. Glycyrrhiza glabra commonly called as liquorice or sweet root is one of the oldest known herbal remedies famous for its vast variety of therapeutic properties. Ethanolic extract of Glycyrrhiza glabra was tested for antifungal activity against Candida albicans, Aspergillus niger, Aspergillus fumigates, Mucor spp and Penicilium marneffei.


Method: Disc diffusion technique was followed for screening anti fungal activity. The discs were loaded with 100µl of ethanolic extracts at different concentrations [500ug/disc,1000ug/disc and 2000µg/disc]. Positive controls used were fluconazole (10 mcg/disc) and amphotericin B (100 units/disc).


Result : After incubation at 28oC for 48 hours, the zone of Inhibition was measured.


Conclusion: The extract at different concentrations showed varying degree of antifungal activity against the micro organisms tested compared to standard.


KEYWORDS: Glycyrrhiza glabra, disc diffusion, zone of inhibition, anti mycotic activity.


Download data is not yet available.


Sheetal verma and S.P.Singh., Current and future status of herbal medicines. Veterinary world, Vol.1 (11): 347 – 350.

Si-Yuan Pan, Si-Bao Chen, Hong-Guang Dong, ZhiLing Yu, Ji-Cui Dong, Zhi-Xian Long, Wang-Fun Fong, et al., New perspectives on Chinese herbal medicine research & development. Evidence Based Complementary and alternative medicine 2011.vol 2011,11 pages.

Olukoga A, Donaldson D. The history of liquorice: the plant, its extract, cultivation, and commercialisation and etymology. J R Soc Health 1998; vol 118:300-304

A.AAl-Qarawi, H.A.Abdel Rahman, B.H.Ali, Liquorice (Glycyrrhiza glabra and the adrenal

kidney -pituitary axis in rats. Food and chemical toxicology, vol 40 [10] : 1525-1527

Fan Y. G.; Shi Z. Q.; He B. L.; Extraction separation and application for glycyrrhizic and glycyrrhetic acid. Natural product Research and Development. 1996, vol 8 : 93â€99

Yang L.; Liu Y. L.; Lin S. Q.; The determination of flavonoid in 6 kinds of licorice root by HPLC. Acta Pharmaceutics Sinica. 1990; 25 : 840-848

Manoj M. Nitalikar, Kailas C. Munde, Balaji V. Dhore, Sajid N. Shikalgar Antibacterial Activities of Glycyrrhiza glabra Root Extract ,International Journal of PharmTech Research Vol.2, [1] :899â€901

Tharkar P.R.,Tatiya A.U., Shinde P.R ., Surana S.J., Patil U.K .,Antifungal Activity of Glycyrrhiza glabra Linn. And Emblica Officinalis Gaertn. by Direct Bioautography Method International Journal of PharmTech Research .,2010; 2 [2] :1547-1549,

Morteza-Semnani K, Saeedi M, Shahnavaz B,Comparison of antioxidant activity of extract from roots of licorice (Glycyrrhiza glabra L.) to commercial antioxidants in 2% hydroquinone

cream. J Cosmet Sci. 2003 Nov-Dec; 54 [6] :551-558.

Anderson DM, Smith WG. The antitussive activity of glycyrrhetinic acid and its derivatives.

J.Pharm.Pharmacol. Jul 1961;vol 13: 396â€404.

Kang HE, Lee MG, Hwang SJ, Kim SC, Lee CH, et al. Liquiritigenin, a flavonoid aglycone from licorice, has a choleretic effect and the ability to induce hepatic transporters and phaseâ€II enzymes. Am J Physiol Gastrointest Liver Physiol. Feb 2009; 296[2]:372â€81

Okimasu E, Moromizato Y, Watanabe S, et al. Inhibition of phospholipase A2 and platelet

aggregation by glycyrrhizin,an anti inflammation drug. Acta Med Okayama1983;37[5] :385â€391.

A.Manja malai, Sneha susan Varghese, Aishwarya Haridas and V.M.Belin grace., Antifungal,anti inflammatory and GC-MS analysis for Bioactive molecules of Tridax procumbens leaf, Asain J of pharmaceutical and clinical research , 2012; 5 [suppl 1]:139 -145

Collins, CH and Lyne, P.M .Microbiological methods, London, Butterworths and co; 1976

Betty A.Forbes., Daniel F.Sahm., Alice S.Weissfeld. Bailey & Scott’s Diagnostic Microbiology 11th edition Mosby;1998

Connie R.Mahon., George Manuselis., Saunder’s Diagnostic Microbiology 2nd Edition. W.B.Saunder’s company;2000

J. G. Colle., A. G. Faster., B. P. Marmion. A. Simmons. Practical Microbiology [Mackie &

Mc Cartney] 14th edition. Elsevier ;2006

N.Jayashree, N.Narayanan, Lata sriram., Antibacterial antifungal and anti mycobacterial studies on some synthetic Dimethoxy flavones, Asain J of pharmaceutical and clinical research , 2012; 5 [suppl 1]:101 -103



How to Cite

GEETHA, R., and R. ANITHA. “IN VITRO EVALUATION OF ANTI MYCOTIC ACTIVITY OF ETHANOLIC EXTRACT OF GLYCYRRHIZA GLABRA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 3, July 2013, pp. 205-6,