SCREENING AND IDENTIFICATION OF HEAVY METAL-TOLERANT ENDOPHYTIC FUNGI LASIODIPLODIA THEOBROMAE FROM BOSWELLIA OVALIFOLIOLATA AN ENDEMIC PLANT OF TIRUMALA HILLS
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i3.16697Abstract
ABSTRACT
Aim: The aim of this study was to evaluate the heavy metal resistance potentiality of endophytic fungi isolated from the leaves of Boswellia ovalifoliolata,
an endemic medicinal plant of Tirumala Hills.
Methods: Initially, isolation of fungal endophytes was carried out. Isolated fungi were screened for the heavy metal resistance against Co, Cd, Cu
and Zn using growth and evaluated their maximum tolerant capacity. Molecular identification of endophytic fungi was carried out by 18S rRNA gene
amplification and Sanger's nucleotide sequencing. Phylogenetic tree was constructed using NCBI Clustal W.
Results: Ten different endophytic fungi were isolated from the leaves of B. ovalifoliolata. Among the isolated endophytic fungi, five showed resistance
to Co, Cd, Cu, and Zn. The most resistant fungus was identified as Lasiodiplodia theobromae based on 18S rRNA gene sequencing.
Conclusions: L. theobromae was isolated from B. ovalifoliolata and identified as one of the useful fungi involved in mycoremediation against heavy
metal toxicity.
Keywords: Heavy metals, Endophytic fungi, Endemic plant, Bioremediation.
Downloads
References
REFERENCES
El-Gendy MM, Hassanein NM, Ibrahim EH, El-Baky DH. Evaluation of some fungal endophytes of plant potentiality as low-cost adsorbents for heavy metals uptake from aqueous solution. Aust J Basic Appl Sci 2011;5(7):466-73.
Hemambika B, Rani MJ, Kannan VR. Biosorption of heavy metals by immobilized and dead fungal cells: A comparative assessment. J Ecol Nat Environ 2011;3(5):168-75.
Granero S, Domingo JL. Levels of metals in soils of Alcalá de Henares, Spain: Human health risks. Environ Int 2002;28(3):159-64.
Kadirvelu K, Senthilkumar P, Thamaraiselvi K, Subburam V. Activated carbon prepared from biomass as adsorbent: Elimination of Ni(II) from aqueous solution. Bioresour Technol 2002;81(1):87-90.
Luo JM, Xiao X, Luo SL. Biosorption of cadmium (II) from aqueous solutions by industrial fungus Rhizopus cohnii. Trans Nonferrous Met Soc China 2010;20:1104-11.
Iskandar NL, Zainudin NA, Tan SG. Tolerance and biosorption of copper (Cu) and lead (Pb) by filamentous fungi isolated from a freshwater ecosystem. J Environ Sci (China) 2011;23(5):824-30.
Shin MN, Shim J, You Y, Myung H, Bang KS, Cho M, et al. Characterization of lead resistant endophytic Bacillus sp. MN3-4 and its potential for promoting lead accumulation in metal hyperaccumulator Alnus firma. J Hazard Mater 2012;199-200:314-20.
He H, Ye Z, Yang D, Yan J, Xiao L, Zhong T, et al. Characterization of endophytic Rahnella sp. JN6 from Polygonum pubescens and its potential in promoting growth and Cd, Pb, Zn uptake by Brassica napus. Chemosphere 2013;90(6):1960-5.
Gadd GM. Fungi and yeast metal accumulation. In: Ehrlich HL, Brierley CL, editors. Microbiol Mineral Recovery. New York: McGraw-Hill; 1990. p. 249-76.
D’Annibale A, Rosetto F, Leonardi V, Federici F, Petruccioli M. Role of autochthonous filamentous fungi in bioremediation of a soil historically contaminated with aromatic hydrocarbons. Appl Environ Microbiol 2006;72(1):28-36.
Selosse MA, Baudoin E, Vandenkoornhuyse P. Symbiotic microorganisms, a key for ecological success and protection of plants. C R Biol 2004;327(7):639-48.
Gadd GM. Geomycology: Biogeochemical transformations of rocks, minerals, metals and radionuclides by fungi, bioweathering and bioremediation. Mycol Res 2007;111(1):3-49.
Valix M, Tang JY, Malik R. Heavy metal tolerance of fungi. Miner Eng2001;14(5):499-505.
Salini G. Pharmacological profile of mangrove endophytes - A review. Int J Pharm Pharmacol Sci 2005;7(1):6-15.
Guba EF. Monograph of the genus Pestalotia de Notaris. Part 1. Phytopathology 1929;19:191-232.
Steyaert RL. Contribution to the study of de Pestalotia de Not. and Monochaetia Sacco (Truncatella gen. Nov. and pestalotiopsis gen. Nov.). Bull Jardin Bot État Bruxelles 1949;19:285-354.
Jeewon R, Liew EC, Simpson JA, Hodgkiss IJ, Hyde KD. Phylogenetic significance of morphological characters in the taxonomy of Pestalotiopsis species. Mol Phylogenet Evol 2003;27(3):372-83.
Maharachchikumbura SS, Guo LD, Chukeatirote E, Bahkali AH, Hyde KD. Pestalotiopsis-morphology, phylogeny, biochemistry and diversity. Fungal Divers 2011;50:167-87.
Zhang YJ, Zhang S, Liu XZ, Wen HA, Wang M. A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Lett Appl Microbiol 2010;51(1):114-8.
Netala VR, Kotakadi VS, Bobbu P, Gaddam SA, Tartte V. Endophytic fungal isolate mediated biosynthesis of silver nanoparticles and their free radical scavenging activity and anti microbial studies. 3 Biotech 2016;6(2):132.
Zhang Z, Schwartz S, Wagner L, Miller W. A greedy algorithm for aligning DNA sequences. J Comput Biol 2000;7(1-2):203-14.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28(10):2731-9.
Iram S, Zaman A, Iqbal Z, Shabbir R. Heavy metal tolerance of fungus isolated from soil contaminated with sewage and industrial wastewater. Pol J Environ Stud 2013;22(3):691-7.
Venkateswarlu N, Sireesha O, Aishwayra S, Vijaya T, Sreeramulu A. Isolation, screening of rhizosphere fungi antagonistic to rice stem rot disease pathogen Sclerotium oryzae catt. Asian J Pharm Clin Res 2015;8(5):54-7.
Ezzouhri L, Castro E, Moya M, Espinola F, Lairini K. Heavy metal tolerance of filamentous fungi isolated from polluted sites in tangier, Morocco. Afr J Microbiol Res 2009;3(2):35-48.
Hong JW, Park JY, Gadd GM. Pyrene degradation and copper and zinc uptake by Fusarium solani and Hypocrea lixii isolated from petrol station soil. J Appl Microbiol 2010;108(6):2030-40.
Salvadori MR, Lepre LF, Ando RA, Oller do Nascimento CA, Corrêa B. Biosynthesis and uptake of copper nanoparticles by dead biomass of Hypocrea lixii isolated from the metal mine in the Brazilian Amazon Region. PLoS One 2013;8(11):e80519.
Deng Z, Zhang R, Shi Y, Hu L, Tan H, Cao L. Characterization of Cd-, Pb-, Zn-resistant endophytic Lasiodiplodia sp. MXSF31 from metal accumulating Portulaca oleracea and its potential in promoting the growth of rape in metal-contaminated soils. Environ Sci Pollut Res Int 2014;21(3):2346-57.
Davis TA, Volesky B, Mucci A. A review of the biochemistry of heavy metal biosorption by brown algae. Water Res 2003;37(18):4311-30.
Mohali S, Burgess TI, Wingfield MJ. Diversity and host association of the tropical tree endophyte Lasiodiplodia theobromae revealed usingsimple sequence repeat markers. For Pathol 2005;35:38596.
Prabavathy D, Nachiyar V. Antimicrobial and antidiabetic activity of an endophytic fungi isolated from Adathoda beddomei. Int J Pharm Pharm Sci 2013;5(3):780-3.
Pandi M, Kumaran RS, Choi YK, Kim HJ, Muthumary J. Isolation and detection of taxol, an anticancer drug produced from Lasiodiplodia theobromae, an endophytic fungus of the medicinal plant Morinda citrifolia. Afr J Biotechnol 2011;10(8):1428-35.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
