EVALUATION OF ANTI-DIABETIC ACTIVTY OF  DIFFERENT EXTRACTS OF MYRISTICA FRAGRANS HOUTT: IN VITRO AND IN SILICO STUDIES

Saranya Sivaraj; Gomathi Kannayiram; Gayathri Dasararaju

doi:10.22159/ajpcr.2017.v10i4.16720

Authors

Saranya Sivaraj
Gomathi Kannayiram
Gayathri Dasararaju

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i4.16720

Abstract

Objective:Â This study is aimed to evaluate the anti-diabetic effect of sequentially extracted (hexane, dichloromethane, ethyl acetate, and ethanol) Myristica fragrans houttÂ (mace) through in vitroÂ and in silicoÂ studies.

Methods:Â The in vitroÂ anti-diabetic effect of the sequentially extracted plant were evaluated for its alpha-amylase inhibitory activity and the potential binding was studied by in silicoÂ studies using SchrÃ¶dinger Maestro.

Results:Â All extracts showed dose dependent alpha-amylase inhibitory effect. At concentration 500 Âµg/ml, all the extracts showed more than 60% inhibition of the alpha-amylase enzyme and the highest inhibition (81.30%) at 500Â Âµg/ml was observed in DCM extract of mace. Potential compounds were identified by in silicoÂ molecular docking studies of alpha-amylase with phytocomponents fromÂ DCM extract. Among the top threeÂ compoundsÂ from virtual screening, induced fit docking studies revealed 2,5-bis(3,4-dimethoxyphenyl)-3,4-dimethyloxolaneÂ possessed better binding affinity when compared with the drug metformin.

Conclusion:Â The obtained in vitroÂ and in silicoÂ results suggest that all extracts of Myristica fragrans can be used successfully for the management of diabetes mellitus.

Keywords: Myristica fragrans, Mace, Sequential extraction, Alpha-amylase, Molecular docking.

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