EXTRACTION OF FLAVONOID FROM VARIOUS PARTS OF COUROUPITAGUIANENSIS AND ITS EFFICACY AGAINST PATHOGENIC BACTERIA

Tahira Akther; Mohd Shahanbaj Khan; Hemalatha S

doi:10.22159/ajpcr.2017.v10i4.16864

Authors

Tahira Akther
Mohd Shahanbaj Khan
Hemalatha S School of Life Sciences, B.S.Abdur Rahman University, Chennai

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i4.16864

Abstract

Objective: Quantitative, qualitative, high performance liquid chromatography (HPLC) analysis of flavonoids content from different parts of CouroupitaÂ guianensis and antibacterial activity against various pathogenic bacteria.

Methods: Different parts of C. guianensis such as leaves, stem bark, flower anther, flower petals, fruit rind, and fruit pulp were collected and usedÂ for extraction Â of active constituents. The phytochemical analysis was conducted to identify the presence of flavonoids in different plant parts. TheÂ amount of flavonoids were analyzed by quantitative analysis. Furthermore, HPLC analysis was performed to isolate and identify the flavonoids fromÂ the different parts. The methanol extract of different plant parts was also used to test the antibacterial efficacy in different human bacterial pathogens.

Results: Flavonoids demonstrate a wide range of biochemical and pharmacological effects. In this study, we identified the flavonoids content inÂ different parts of C. guianensis. The HPLC analysis has significantly proved the presence of flavonoids in different plants parts and amount of flavonoidsÂ differs in different parts of the plant. The results also showed the significant efficacy of its extract to the different pathogenic bacterial strains.

Conclusion: Our study suggested that C. guianensis is a richer source of flavonoids and due to a higher amount of flavonoids; it may have enormousÂ potential to scavenge the free radicals, oxidative damage of cell during different stresses including bacterial infections. We also suggested that HPLCÂ analysis is efficient method to isolate and identify the different compounds and study can further extend to identify and isolate the different novelÂ compounds from C. guianensis.

Keywords: Couroupita guianensis, High performance liquid chromatography, Flavonoids, Antibacterial activity.

Downloads

Download data is not yet available.

References

Venkatesh S, Reddy GD, Reddy BM, Ramesh M, Rao AV. Antihyperglycemic activity of Caralluma attenuata. Fitoterapia 2003;74:274-9.

Suba V, Murugesan T, Rao RB, Ghosh L, Pal M, Mandal SC, et al. Antidiabetic potential of Barleria lupulina extract in rats. Fitoterapia 2004;75(3):1-4.

WHO. The WHO Expert Committee on Diabetes Mellitus, Technical Report Series. Geneva: World health Organization; 1980. p. 646.

Pandey UV. Ayurvedic approach to diabetes mellitus and its management by indigenous resources. In: Bajaj JS, editor. Diabetes Mellitus in Developing Countries. New Delhi: Interprint; 1980. p. 375-7.

Sanz-Biset J, Campos-de-la-Cruz J, EpiquiÃ©n-Rivera MA, CaÃ±igueral S. A first survey on the medicinal plants of the Chazuta valley (Peruvian Amazon). J Ethnopharmacol 2009;122(2):333-62.

Kokat CK. Practical Pharmacognosy. 2nd ed. Vol. 35. New Delhi: Vallabh Prakashan; 1998. p. 193-208.

Boyum A. Isolation of Mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Chin Lab Invest Suppl 1968;97:77-89.

Desal T, Golatakar SG, Rane JB, Ambaye RY, Kamath VR. Larvicidal property of Couroupita guianensis Aubl. Indian Drugs 2003;40:484-6.

Pradhan D, Panda PK, Tripathi G. Evaluation of immunomodulatory activity of methanolic extract of Couroupita guianensis Aublet. Flowers in rats. Nat Prod Radiance 2009;8(1):37-42.

Kavitha R, Kamalakannan P, Deepa T, Elamathi R, Sridhar S, Kumar JS. In vitro antimicrobial activity and phytochemical analysis of Indian medicinal plant Couroupita guianensis Aubl. J Chem Pharm Res 2011;3(6):115-21.

Premanathan M, Radhakrishnan S, Kulangiappar K, Singaravelu G, Thirumalaiarasu V, Sivakumar T, et al. Antioxidant & anticancer activities of isatin (1H-indole-2,3-dione), isolated from the flowers of Couroupita guianensis Aubl. Indian J Med Res 2012;136(5):822-6.

Rane JB, Vahanwala SJ, Golatakar SG, Khadse BG, Ambaye R. Chemical examination of the flowers of Couroupita guianensis Aubl. Indian J Pharm Sci 1994;56:72-3.

Rane JB, Vahanwala SJ, Golatkar SG, Ambaye RY, Khadse BG. Chemical examination of the flowers of Couroupita guianensis Aubl. Indian J Pharm Sci 2001;63(1):72-3.

Gamble JS. The Flora of the Presidency of Madras. London: Adlard and Sonâ€™s Ltd.; 1935.

Mathew KM. The Flora of Tamil Nadu and Karnatic Part 1-3. Medicinal Plants of Kolli Hills on Mitogen-Stimulated Proliferation of the Human Peripheral. Thirucharappalli, Tamil Nadu: The Rapinat Herbarium; 1981-1984.16. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.

Malik A, Ahmad AR. Determination of phenolic and flavonoid contents of ethanolic extract of Kanunang leaves (Cordia myxa L.). Int J PharmTech Res 2015;7(2):243-6.

Jayaprakash R, Sha SK, Hemalatha S, Easwaramoorthy D. QSAR, brine shrimp lethal assay and antimicrobial studies on synthesized L-tryptophan-2, 4-dihydroxy benzaldehyde schiff base. Int J ChemTech Res 2016;9(6):48-54.

Massiot G, Chen X, Lavaud C, Men-Olivier LL, Delaude C, Viari A, et al. Saponins from stem bark of Petersianthus macrocarpus. Phytochemistry 1992;31(10):3571-6.

Das MC, Mahato SB. Triterpenoidsapogenols from the leaves of Careya arborea: Structure of careyagenolide. Phytochemistry 1982;21(8):2069-73.

Parikh NH, KothariCS. Phytochemical analysis and total phenolic and flavonoid contents determination of methanolic extract of Ocimum basilicum L seed. Int J PharmTech Res 2016;9(4):215-9.

Crublet ML, Long C, SÃ©venet T, Hadi HA, Lavaud C. Acylated flavonol glycosides from leaves of Planchonia grandis. Phytochemistry 2003;64(2):589-94.

Goveas SW, Abraham A. Evaluation of antimicrobial and antioxidant activity of stem and leaf extracts of Coscinium fenestratum. Asian J Pharm Clin Res 2013;6(3):218-21.

Reetika S, Nishi K, Mayank G, Gopal N. Qualitative characterization of phytochemicals and in vitro antimicrobial evaluation of leaf extract of Couroupita guianensis Aubl. - A threatened medicinal tree. Int J Pharm Pharm Sci 2012;4:126-30.

Velliangiri P, Subban R. Quantification of quercetin and stigmasterol of Couroupita guianensis Aubl by HPTLC method and in-vitro cytototoxic activity by MTT assay of the methanol extract against Hela, NIH 3T3 and HEPG2 cancer cell. Int J Pharm Pharm Sci 2012;4:126-30.