MOLECULAR CHARACTERIZATION AND DRUG RESISTANCE OF ENTEROPATHOGENIC BACTERIA ISOLATED FROM READY TO EAT FOOD IN LUDHIANA
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i5.17918Keywords:
Pathogenic organisms, Ready-to-eat, Food, Toxic syndrome, LudhianaAbstract
Objective: The study was conducted to investigate the enteropathogenic bacteria from the most common street food sold in Ludhiana at different
places.
Methods: A total of 90 samples of street food samples were randomly collected from four locations of Ludhiana. Samples investigated for the presence of bacteria using appropriate selective and differential culture media. The spread plate technique was used to analyze the samples.
Results: A total of 90 samples were analyzed, and the total viable counts of the bacterial population in all food samples were in the range of
2.0 × 103 to 1.8 × 106 CFU/g. The nonvegetarian food samples recorded the 1.8 × 106 CFU/g; however, other street foods also have highest total viable
microbial count 1.1 × 106 CFU/g. The total coliform count ranged from 1.9 × 103 to 9.4 × 105 CFU/g, and pani-puri has significant count. The range of
Staphylococci count was 1.9 × 103 to 5.3 × 103 CFU/g, chicken tandoori sample also high count bacteria. The incidence of manifestations of the seven
pathogenic bacteria isolated from all street food Proteus sp. (2%) Staphylococci were the most predominant. This was followed by Salmonella (6%), Staphylococcus aureus (14%), Klebsiella sp. (5%), and Enterococcus sp. (2%) were least predominant.
Conclusion: The study exhibited contagion may be due to poor techniques of food preparation adopted by the street food vendors. This is of community health worry as these bacteria are known as food-borne illnesses and toxic syndrome.
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