GREEN PHYTO-SYNTHESIS OF GOLD NANOPARTICLES USING ACHYRANTHES ASPERA LINN SEED-EPICOTYLS LAYER EXTRACTS AND ITS ANTICANCER ACTIVITY
Abstract
In the recent past decades, green phyto-process for the synthesis of metal incorporated nanoparticles has been evolving into an imperative branch of nanotechnology. We have reported a rapid, expedient and extracellular method for the synthesis of gold nanoparticles by reducing gold chloride with the help of aqueous seed-epicotyls layer extracts of Achyranthes aspera Linn (Amarantheceae). This approach is simple, economic, stable for a long time, reproducible at room temperature and is synthesized in an eco-friendly mode to obtain a self-assembly of gold nanoparticles (AuNPs). The resulting gold nanoparticles were characterized using UV-Visible absorption spectroscopy, Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) and FT-IR spectroscopic techniques. The anticancer activity of the gold nanoparticles was studied against HeLa (Cervical) cancer cell lines. We herein report for the first time, Achyranthes aspera Seed-Epicotyls assisted synthesis of biogenic gold nanoparticles; the nanoparticles are conspicuously smaller and better faceted compared with those synthesized by A. aspera leaf extracts previously reported. Synthesized gold nanoparticles showed potent anticancer activity at 50μg/ml concentration against cervical cancer cell lines.
Keywords: Gold nanoparticles, anticancer activity, Achyranthes Aspera Linn. Aqueous leaves Extract, SEM, XRD, HeLa cancer cell linesDownloads
References
Maximizing DNA loading on a range of gold nanoparticle sizes. J Am Chem Soc 1998;120:1964.
Wohltjen H, Snow AW. Colloidal metal - Insulator-metal ensemble chemiresistor sensor. Anal Chem 1998;70:2859.
Demaille C, Brust M, Tsionsky M, Bard AJ. Catalysis with transition metal nanoparticles in colloidal solution: Nanoparticle biosensor based on integrated assembly of enzymes and gold nanoparticles. Anal Chem 1997;69:2328.
Buzea C, Pacheco II, Robbie K. Nanomaterials and nanoparticles: Sources and toxicity. Biointerphases 2007;2(4):MR17-71.
Bisen DP, Sharma R, Brahme N, Tamrakar R. Effect of temperature on the synthesis of Cds: Mn doped nanoparticles. Chalcogenide Lett 2009;6(9):431.
Eychmüller A, Rogach AL. Chemistry and photophysics of thiol-stabilized II-VI semiconductor nanocrystals. Pure Appl Chem 2000;72(1–2):188.
Franzblau SG, Witzig RS, McLaughlin JC, Torres P, Madico G, Hernandez A, et al. In vitro antimycobacterial activities of Physalis angulata L. J Clin Microbiol 1998;36:366.
Girach RD, Khan AS. Ethnomedicinal uses of Achyranthes aspera leaves in Orissa (India). Int J Pharmacogn 1992;30:115.
Gokhale AB, Damre AS, Kulkami KR, Saraf MN. Preliminary evaluation of anti-inflammatory and anti-arthritic activity of S. lappa, A. speciosa and A. aspera. Phytomedicine 2002;9(5):433-7.
Harborne JB. Phytochemical Methods. London: Chapman and Hall; 1973. p. 52-105.
Kapoor VK, Harkishan S. Investigation of Achyranthes aspera Linn. Indian J Pharmacol 1967;29:285-8.
Manandhar NP. Plants and People of Nepal. Oregon: Timber Press; 2002.
Guzmán MG, Dille J, Godet S. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int J Chem Biol Eng 2009;2(3):111.
Mazur M. Electrochemically prepared silver nanoflakes and nanowires. Electrochem Commun 2004;6:403.
McCloud T, Nemee J, Muschik G, Sheffield H, Quesenberry P, Suffness M. Extraction of bioactive molecules from plants. Proceedings of the International Congress on Natural Products Research, Park City, UT; 1988. p. 17-21.
Nadkarni K, Nadkarni AK. Indian Materia Medica. Vol. 1. Bombay: Popular Prakashan; 1976. p. 550.
Londonkar RM, Chinnappa Reddy V, Abhay kumar K. Potential antibacterial and antifungal activity of Achyranthes aspera L. Rec Res Sci Technol 2011;3:57.
Mohammed Fayaz A, Girilal M, Venkatesan R, Kalaichelvan PT. Biosynthesis of anisotropic gold nanoparticles using Maduca longifolia extract and their potential in infrared absorption. Colloids Surf B 2011;88(1):291.
Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol Prog 2006;22(2):583.
Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, et al. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology 2007;18(10):104.
Ankamwar B. Chaudhary M, Sastry M. Gold nanotriangles biologically synthesized using tamarind leaf extract and potential application in vapor sensing. Synth Reactivity Inorgan Metal Organ Nano Metal Chem 2005;35(1):26.
Shankar SS, Rai A, Ahmad A, Sastry M. Controlling the optical properties of lemongrass extract synthesized gold nanotriangles and potential application in infrared-absorbing optical coatings. Chem Mater 2005;17(3):572.
Shankar SS, Rai A, Ahmad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 2004;275(2):496-502.
Ankamwar B, Damle C, Ahmad A, Sastry M. Biosynthesis of gold and silver nanoparticles using Emblica officinalis fruit extract, their phase transfer and transmetallation in an organic solution. J Nanosci Nanotechnol 2005;5(10):1665-71.
Raghunandan D, Bedre MD, Basavaraja S, Sawle B, Manjunath SY, Venkataraman A. Rapid biosynthesis of irregular shaped gold nanoparticles from macerated aqueous extracellular dried clove buds (Syzygium aromaticum) solution. Colloids Surf B Biointerfaces 2010;79(1):235-40.
Ratra PS, Misra KC. Sea sand variation in chemical composition of Achyranthes aspera and Achyranthes bidentata. Indian Forester 1970;96:372-5.
Srivastav S, Singh P, Mishra G, Jha KK, Khosa RL. Achyranthes aspera - An important medicinal plant: A review. J Nat Prod Plant Resour 2011;1(1):11-4.
Carnichael J, Degraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium-based semiautomated colorimetrie asay: Assessment of radiosensitvity. Cancer Res 1987;47:936.
Mosmann T. Rapid colorimetrie asay for celular growth and survival: Application to proliferation and cytotoxicity asays. J Immunol Methods 1983;65:55.
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